hrde-1 Argonaute protein hrde-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: hrde-1
Official Full Name: Argonaute protein hrde-1
Primary source: WormBase:WBGene00007624
Locus tag: CELE_C16C10.3
See related: AllianceGenome:WB:WBGene00007624
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable RNA endonuclease activity; miRNA binding activity; and single-stranded RNA binding activity. Involved in germ cell development and regulatory ncRNA-mediated post-transcriptional gene silencing. Located in nucleus. Part of RNAi effector complex. Is expressed in germ cell; germ line; and in male. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See hrde-1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 7

Sequence:: Chromosome: III; NC_003281.10 (4172337..4176159, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HRDE-2 drives small RNA specificity for the nuclear Argonaute protein HRDE-1.
Title: HRDE-2 drives small RNA specificity for the nuclear Argonaute protein HRDE-1.
The nuclear Argonaute HRDE-1 directs target gene re-localization and shuttles to nuage to promote small RNA-mediated inherited silencing.
Title: The nuclear Argonaute HRDE-1 directs target gene re-localization and shuttles to nuage to promote small RNA-mediated inherited silencing.
In the absence of piRNAs, histone mRNAs are misrouted into the nuclear RNAi pathway involving the Argonaute HRDE-1, concurrent with a reduction in the expression of many histone mRNAs.
Title: Widespread roles for piRNAs and WAGO-class siRNAs in shaping the germline transcriptome of Caenorhabditis elegans.
HRDE-1 physically interacts with the helicase EMB-4.
Title: The Helicase Aquarius/EMB-4 Is Required to Overcome Intronic Barriers to Allow Nuclear RNAi Pathways to Heritably Silence Transcription.
Nuclear import of the secondary siRNAs by the Argonaute HRDE-1 leads to the production of a distinct class of small RNAs that map throughout the transcript, which we term tertiary siRNAs.
Title: Tertiary siRNAs mediate paramutation in C. elegans.
results establish that the Argonaute protein HRDE-1 directs gene-silencing events in germ-cell nuclei that drive multigenerational RNAi inheritance and promote immortality of the germ-cell lineage
Title: A nuclear Argonaute promotes multigenerational epigenetic inheritance and germline immortality.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C16C10.3 (e-PCR)
- UniSTS:306487

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in germ cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNAi effector complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Argonaute protein hrde-1

NP_497834.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

4172337..4176159 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_065433.8 → NP_497834.1 Argonaute protein hrde-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_497834.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q09249

Conserved Domains (4) summary

cd02826
Location:540 → 974

Piwi-like; Piwi-like: PIWI domain. Domain found in proteins involved in RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The ...

cd02846
Location:389 → 480

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

pfam08648
Location:315 → 379

DUF1777; Protein of unknown function (DUF1777)

pfam16488
Location:517 → 556

ArgoL2; Argonaute linker 2 domain