mel-26 Protein maternal effect lethal 26;Protein roadkill [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: mel-26
Official Full Name: Protein maternal effect lethal 26;Protein roadkill
Primary source: WormBase:WBGene00003209
Locus tag: CELE_ZK858.4
See related: AllianceGenome:WB:WBGene00003209
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables several functions, including cullin family protein binding activity; identical protein binding activity; and protein kinase binding activity. Involved in several processes, including cytoskeleton organization; positive regulation of TORC1 signaling; and regulation of mitotic spindle elongation. Located in several cellular components, including cleavage furrow; condensed chromosome; and sarcomere. Is expressed in germ line and muscle cell. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See mel-26 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 6

Sequence:: Chromosome: I; NC_003279.8 (9132538..9135753)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

KLHL22 plays a conserved role to mediate the activation of mTORC1 and downstream events in mammals and nematodes; depletion of MEL-26, the Caenorhabditis elegans orthologue of KLHL22, extends worm lifespan
Title: KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.
study demonstrates that the level of MEI-1 protein is reduced in an unc-89 mutant, indicating that the normal role of UNC-89 is to inhibit the CUL-3/MEL-26 complex toward MEI-1 in striated muscle
Title: UNC-89 (obscurin) binds to MEL-26, a BTB-domain protein, and affects the function of MEI-1 (katanin) in striated muscle of Caenorhabditis elegans.
MEI-1 is the only essential target for MEL-26, and possibly for the E3 ubiquitin ligase CUL-3, but the upstream ubiquitin ligase activating enzyme RFL-1 has additional essential targets
Title: Levels of the ubiquitin ligase substrate adaptor MEL-26 are inversely correlated with MEI-1/katanin microtubule-severing activity during both meiosis and mitosis.
FIGL-1 by the CUL-3MEL-26 E3 ligase spatially restricts FIGL-1 function to mitotic cells, where it is required for correct progression through mitosis.
Title: The AAA-ATPase FIGL-1 controls mitotic progression, and its levels are regulated by the CUL-3MEL-26 E3 ligase in the C. elegans germ line.
KO8F11.3 regulates mel-26 in the helminth embryo.
Title: CIF-1, a shared subunit of the COP9/signalosome and eukaryotic initiation factor 3 complexes, regulates MEL-26 levels in the Caenorhabditis elegans embryo.
the identification of a large family of BTB-domain protein MEL-26 and its genetic target MEI-1 as substrate-specific adaptors for C. elegans CUL-3
Title: BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3.
The BTB-containing protein MEL-26 is a component required for degradation of MEI-1 in vivo; importantly, MEL-26 specifically interacts with CUL-3 and MEI-1 in vivo and in vitro, and displays properties of a substrate-specific adaptor
Title: The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase.
MEL-26 not only acts as a substrate-specific adaptor within the MEL-26/CUL-3 complex, but also promotes cytokinesis by a CUL-3- and microtubule-independent mechanism.
Title: The BTB protein MEL-26 promotes cytokinesis in C. elegans by a CUL-3-independent mechanism.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ZK858.4 (e-PCR)
- UniSTS:306872

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables cullin family protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cellular response to L-leucine	ISS Inferred from Sequence or Structural Similarity more info
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of autophagy	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of TORC1 signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of cell growth	ISS Inferred from Sequence or Structural Similarity more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of mitotic spindle elongation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in striated muscle myosin thick filament assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in I band	IDA Inferred from Direct Assay more info	PubMed
located_in I band	IEA Inferred from Electronic Annotation more info
located_in M band	IDA Inferred from Direct Assay more info	PubMed
located_in M band	IEA Inferred from Electronic Annotation more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cleavage furrow	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in lysosome	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: Protein maternal effect lethal 26;Protein roadkill

NP_001263463.1

Confirmed by transcript evidence

NP_492449.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

9132538..9135753

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001276534.2 → NP_001263463.1 Protein roadkill [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001263463.1

Status: REVIEWED

UniProtKB/TrEMBL

K8F7T2

Conserved Domains (4) summary

smart00225
Location:202 → 300

BTB; Broad-Complex, Tramtrack and Bric a brac

cd14734
Location:300 → 366

SPOP_C; BACK-like Domain C-terminal to BTB in speckle-type POZ protein (SPOP) and similar proteins

pfam00651
Location:191 → 297

BTB; BTB/POZ domain

cl02446
Location:38 → 157

MATH; MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane ...
NM_060048.12 → NP_492449.2 Protein maternal effect lethal 26 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_492449.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94139, Q94420

Conserved Domains (4) summary

smart00225
Location:202 → 300

BTB; Broad-Complex, Tramtrack and Bric a brac

cd14734
Location:300 → 362

SPOP_C; BACK-like Domain C-terminal to BTB in speckle-type POZ protein (SPOP) and similar proteins

pfam00651
Location:191 → 297

BTB; BTB/POZ domain

cl02446
Location:38 → 157

MATH; MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane ...