Acadm acyl-Coenzyme A dehydrogenase, medium chain [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Acadmprovided by MGI
Official Full Name: acyl-Coenzyme A dehydrogenase, medium chainprovided by MGI
Primary source: MGI:MGI:87867
See related: Ensembl:ENSMUSG00000062908 AllianceGenome:MGI:87867
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MCAD
Summary: This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice, deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple, intronless pseudogenes. [provided by RefSeq, Nov 2012]
Expression: Broad expression in heart adult (RPKM 179.5), liver E18 (RPKM 140.4) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 H3; 3 78.77 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (153627990..153650280, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (153922353..153944643, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transcriptome analysis suggests a compensatory role of the cofactors coenzyme A and NAD(+) in medium-chain acyl-CoA dehydrogenase knockout mice.
Title: Transcriptome analysis suggests a compensatory role of the cofactors coenzyme A and NAD(+) in medium-chain acyl-CoA dehydrogenase knockout mice.
Quantitative Proteomics of Th-MYCN Transgenic Mice Reveals Aurora Kinase Inhibitor Altered Metabolic Pathways and Enhanced ACADM To Suppress Neuroblastoma Progression.
Title: Quantitative Proteomics of Th-MYCN Transgenic Mice Reveals Aurora Kinase Inhibitor Altered Metabolic Pathways and Enhanced ACADM To Suppress Neuroblastoma Progression.
A high-fat diet increases adiposity but maintains mitochondrial oxidative enzymes (MCAD/citrate synthase) without affecting development of heart failure with pressure overload.
Title: A high-fat diet increases adiposity but maintains mitochondrial oxidative enzymes without affecting development of heart failure with pressure overload.
MCAD deficiency in mice leads to specific changes in hepatic carbohydrate management on exposure to metabolic stress.
Title: Disturbed hepatic carbohydrate management during high metabolic demand in medium-chain acyl-CoA dehydrogenase (MCAD)-deficient mice.
The MCAD-/- mice developed an organic aciduria and fatty liver, and showed profound cold intolerance at 4 degrees C with prior fasting.
Title: Medium-chain acyl-CoA dehydrogenase deficiency in gene-targeted mice.
MCAD is reduced in liver, heart & kidney in lipopolysaccharide-induced acute phase response; binding liver nuclear extracts to ERRalpha response element found in promoter region of MCAD was decreased during APR, suggesting less transcription of MCAD
Title: Suppression of estrogen-related receptor alpha and medium-chain acyl-coenzyme A dehydrogenase in the acute-phase response.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH136234 (e-PCR)
- UniSTS:210228

D3Mit88 (e-PCR), detects polymorphism
- UniSTS:129857

Acadm (e-PCR), detects polymorphism
- UniSTS:506700

ACADM (e-PCR)
- UniSTS:266352

Acadm (e-PCR), detects polymorphism
- UniSTS:502700

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acyl-CoA dehydrogenase activity	ISO Inferred from Sequence Orthology more info
enables acyl-CoA dehydrogenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables fatty-acyl-CoA binding	ISO Inferred from Sequence Orthology more info
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables flavin adenine dinucleotide binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables isomerase activity	ISO Inferred from Sequence Orthology more info
enables medium-chain fatty acyl-CoA dehydrogenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables medium-chain fatty acyl-CoA dehydrogenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables medium-chain fatty acyl-CoA dehydrogenase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cardiac muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carnitine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in carnitine biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within carnitine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carnitine metabolic process, CoA-linked	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carnitine metabolic process, CoA-linked	ISO Inferred from Sequence Orthology more info
involved_in fatty acid beta-oxidation	ISO Inferred from Sequence Orthology more info
involved_in fatty acid beta-oxidation	ISS Inferred from Sequence or Structural Similarity more info
involved_in fatty acid beta-oxidation using acyl-CoA dehydrogenase	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fatty acid beta-oxidation using acyl-CoA dehydrogenase	ISO Inferred from Sequence Orthology more info
involved_in fatty acid beta-oxidation using acyl-CoA dehydrogenase	ISS Inferred from Sequence or Structural Similarity more info
involved_in glycogen biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within liver development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in medium-chain fatty acid catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in medium-chain fatty acid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in medium-chain fatty acid catabolic process	ISO Inferred from Sequence Orthology more info
involved_in medium-chain fatty acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in medium-chain fatty acid metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within organic acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within post-embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gluconeogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to cold	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon	IEA Inferred from Electronic Annotation more info
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrial matrix	ISO Inferred from Sequence Orthology more info
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: medium-chain specific acyl-CoA dehydrogenase, mitochondrial

NP_031408.1

EC 1.3.8.7

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007382.5 → NP_031408.1 medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor

See identical proteins and their annotated locations for NP_031408.1

Status: REVIEWED

Source sequence(s)

AC087184, AK146444, BB745012, CX232934

Consensus CDS

CCDS17924.1

UniProtKB/Swiss-Prot

P45952, Q64235

UniProtKB/TrEMBL

Q91WS8

Related

ENSMUSP00000072483.7, ENSMUST00000072697.13

Conserved Domains (2) summary

cd01157
Location:41 → 418

MCAD; Medium chain acyl-CoA dehydrogenase

COG1960
Location:39 → 420

CaiA; Acyl-CoA dehydrogenase related to the alkylation response protein AidB [Lipid transport and metabolism]