Ano2 anoctamin 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ano2provided by MGI
Official Full Name: anoctamin 2provided by MGI
Primary source: MGI:MGI:2387214
See related: Ensembl:ENSMUSG00000038115 AllianceGenome:MGI:2387214
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tmem16b
Summary: Enables identical protein binding activity and intracellularly calcium-gated chloride channel activity. Acts upstream of or within chloride transport and establishment of localization in cell. Located in neuron projection; non-motile cilium; and plasma membrane. Is active in glutamatergic synapse and presynaptic membrane. Is expressed in dorsal root ganglion; epidermis; medulla oblongata basal plate mantle layer; olfactory epithelium; and spinal cord mantle layer. Orthologous to human ANO2 (anoctamin 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in testis adult (RPKM 1.4), placenta adult (RPKM 0.4) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 F3; 6 59.58 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (125667382..126017115)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (125690419..126040133)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
Title: Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
TMEM16b regulates action potential firing in lateral septum and aggression in male mice.
Title: TMEM16B Calcium-Activated Chloride Channels Regulate Action Potential Firing in Lateral Septum and Aggression in Male Mice.
Ano2/TMEM16B is a Ca2+-activated chloride channel in vagal afferents of nodose neurons and a major determinant of CCK-induced satiety, body weight control, and energy expenditure, making it a potential therapeutic target in obesity.
Title: TMEM16B determines cholecystokinin sensitivity of intestinal vagal afferents of nodose neurons.
Here the authors report that Ano2 knockout mice exhibit impaired anxiety-related behaviors and context-independent fear memory, thus implicating TMEM16B in anxiety modulation.
Title: TMEM16B regulates anxiety-related behavior and GABAergic neuronal signaling in the central lateral amygdala.
Study provides the first in vivo evidence toward an alternative or additional role for Ano2 in the olfactory transduction cascade, where it may serve as a feedback mechanism to clamp olfactory receptor neurons spike output.
Title: Calcium-activated chloride channels clamp odor-evoked spike activity in olfactory receptor neurons.
Results of behavioral studies obtained from Ano2 (-/-) mice, a mouse line that was previously shown to lack this particular mode of ionic plasticity. The animals display motor coordination deficits that constitute a condition of mild ataxia. Moreover, motor learning is severely impaired in Ano2 (-/-) mice, suggesting cerebellar dysfunction.
Title: Impaired Motor Coordination and Learning in Mice Lacking Anoctamin 2 Calcium-Gated Chloride Channels.
Results suggest that the anoctamin 2-mediated amplification mechanism enables mice to track weak, unfamiliar olfactory cues.
Title: Tracking of unfamiliar odors is facilitated by signal amplification through anoctamin 2 chloride channels in mouse olfactory receptor neurons.
TMEM16B controls AP firing and ensures correct glomerular targeting of olfactory sensory neurons s expressing I7.
Title: The Ca2+-activated Cl- channel TMEM16B regulates action potential firing and axonal targeting in olfactory sensory neurons.
Loss of TMEM16B expression resulted in the absence of Calcium-activated Chloride channels in inferior Olive neurons, leading to markedly diminished action potential firing of IO neurons. TMEM16B knockout mice. Moreover, exhibited severe cerebellar motor learning deficits.
Title: Inferior Olivary TMEM16B Mediates Cerebellar Motor Learning.
The siRNA knock-down suggests that Ano2 contributes to Ca(2+)-dependent chloride conductance in the RPE.
Title: Anoctamin2 (TMEM16B) forms the Ca(2+)-activated Cl(-) channel in the retinal pigment epithelium.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (3) 1 citation
Transposon induced (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

31.MMHAP94FLC4.seq (e-PCR)
- UniSTS:123965

06.MMHAP83FLB3.seq (e-PCR)
- UniSTS:122869

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables chloride channel activity	TAS Traceable Author Statement more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables intracellularly calcium-gated chloride channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables intracellularly calcium-gated chloride channel activity	IDA Inferred from Direct Assay more info	PubMed
enables intracellularly calcium-gated chloride channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables intracellularly calcium-gated chloride channel activity	ISO Inferred from Sequence Orthology more info
NOT enables phospholipid scramblase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables protein dimerization activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in chloride transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in chloride transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chloride transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within chloride transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment of localization in cell	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of chloride channel complex	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in non-motile cilium	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: anoctamin-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001364562.1 → NP_001351491.1 anoctamin-2 isoform 2

Status: VALIDATED

Source sequence(s)

KC164759

UniProtKB/TrEMBL

M1GTU4, M1H0B0

Conserved Domains (2) summary

pfam04547
Location:351 → 936

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:165 → 348

Anoct_dimer; Dimerisation domain of Ca+-activated chloride-channel, anoctamin
NM_001364563.1 → NP_001351492.1 anoctamin-2 isoform 3

Status: VALIDATED

Source sequence(s)

KC164761

UniProtKB/TrEMBL

M1GNA0, M1GRW0

Conserved Domains (2) summary

pfam04547
Location:289 → 874

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:103 → 286

Anoct_dimer; Dimerisation domain of Ca+-activated chloride-channel, anoctamin
NM_153589.2 → NP_705817.2 anoctamin-2 isoform 1

See identical proteins and their annotated locations for NP_705817.2

Status: VALIDATED

Source sequence(s)

AK044763, BC033409, BC060368

Consensus CDS

CCDS39643.2

UniProtKB/Swiss-Prot

C4N788, Q8C8N6, Q8CFW1

UniProtKB/TrEMBL

M1GTU4

Related

ENSMUSP00000125303.2, ENSMUST00000160496.8

Conserved Domains (2) summary

pfam04547
Location:351 → 940

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:91 → 348

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

125667382..126017115

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006506117.4 → XP_006506180.1 anoctamin-2 isoform X2

UniProtKB/TrEMBL

M1GNA0

Conserved Domains (2) summary

pfam04547
Location:289 → 878

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:29 → 286

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006506115.3 → XP_006506178.1 anoctamin-2 isoform X1

UniProtKB/TrEMBL

M1GNA0

Conserved Domains (2) summary

pfam04547
Location:306 → 895

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:46 → 303

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin
XM_006506119.3 → XP_006506182.1 anoctamin-2 isoform X3

See identical proteins and their annotated locations for XP_006506182.1

UniProtKB/TrEMBL

M1GNA0

Conserved Domains (2) summary

pfam04547
Location:262 → 851

Anoctamin; Calcium-activated chloride channel

pfam16178
Location:2 → 259

Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin