Myf6 myogenic factor 6 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Myf6provided by MGI
Official Full Name: myogenic factor 6provided by MGI
Primary source: MGI:MGI:97253
See related: Ensembl:ENSMUSG00000035923 AllianceGenome:MGI:97253
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MRF4; bHLHc4; herculin
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific. Acts upstream of or within several processes, including regulation of DNA-templated transcription; skeletal muscle cell differentiation; and somitogenesis. Predicted to be located in cytosol; mitotic spindle; and nucleoplasm. Is expressed in several structures, including esophagus; ganglia; limb; paraxial mesenchyme; and skeletal musculature. Used to study alveolar rhabdomyosarcoma. Human ortholog(s) of this gene implicated in congenital structural myopathy. Orthologous to human MYF6 (myogenic factor 6). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in mammary gland adult (RPKM 1.9), limb E14.5 (RPKM 1.2) and 7 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 D1; 10 55.96 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (107328709..107330584, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (107492848..107494723, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Myf6/MRF4 is a myogenic niche regulator required for the maintenance of the muscle stem cell pool.
Title: Myf6/MRF4 is a myogenic niche regulator required for the maintenance of the muscle stem cell pool.
findings identified miR-374b directly targets Myf6 and negatively regulates myogenesis
Title: MicroRNA, miR-374b, directly targets Myf6 and negatively regulates C2C12 myoblasts differentiation.
Mrf4 has a role in priming embryonic founder cells to become adult muscle stem cells
Title: Embryonic founders of adult muscle stem cells are primed by the determination gene Mrf4.
Intrinsic muscle factor MRF4 plays an important role in maintenance of neuromuscular junctions.
Title: Loss of synaptic vesicles from neuromuscular junctions in aged MRF4-null mice.
Data demonstrated that Myf6 is Tceal7 upstream transactivators using transcriptional assays.
Title: Myogenic regulatory factors transactivate the Tceal7 gene and modulate muscle differentiation.
MRF4 targets the proximal region of M-cadherin promoter and bind the E4-box.
Title: Myogenic regulatory factors regulate M-cadherin expression by targeting its proximal promoter elements.
Hox genes produce global patterns in the axial skeleton; Myf5 and Myf6 have roles in rib formation
Title: Evidence for a myotomal Hox/Myf cascade governing nonautonomous control of rib specification within global vertebral domains.
Taken together, these results indicate that MRF4 and MyoD play competitive roles in myogenesis by stimulating cell proliferation and differentiation, respectively.
Title: Opposite roles of MRF4 and MyoD in cell proliferation and myogenic differentiation.
an enhancer element directs MRF4 gene expression primarily in fast muscle fibers
Title: A fast fiber enhancer exists in the muscle regulatory factor 4 gene promoter.
skeletal muscle is present in the new Myf5:Myod double-null mice only when Mrf4 expression is not compromised
Title: Mrf4 determines skeletal muscle identity in Myf5:Myod double-mutant mice.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (8) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:548383

NoName (e-PCR)
- UniSTS:493890

Myf6 (e-PCR), detects polymorphism
- UniSTS:143946

Myf6 (e-PCR), detects polymorphism
- UniSTS:143947

Myf6 (e-PCR), detects polymorphism
- UniSTS:143948

Myf6 (e-PCR), detects polymorphism
- UniSTS:463952

PMC133893P2 (e-PCR)
- UniSTS:270679

X59060 (e-PCR)
- UniSTS:180115

Myf6 (e-PCR), detects polymorphism
- UniSTS:545962

Myf6 (e-PCR), detects polymorphism
- UniSTS:265669

Myf6 (e-PCR), detects polymorphism
- UniSTS:233981

RH94559 (e-PCR)
- UniSTS:119807

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	TAS Traceable Author Statement more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within muscle tissue morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of myoblast differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of skeletal muscle fiber development	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in skeletal muscle cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within skeletal muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within skeletal muscle tissue development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within skeletal muscle tissue development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within skeletal muscle tissue development	TAS Traceable Author Statement more info	PubMed
involved_in skeletal muscle tissue regeneration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within somitogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: myogenic factor 6

Names: muscle-specific regulatory factor 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.