Eno1 enolase 1, alpha non-neuron [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Eno1provided by MGI
Official Full Name: enolase 1, alpha non-neuronprovided by MGI
Primary source: MGI:MGI:95393
See related: Ensembl:ENSMUSG00000063524 AllianceGenome:MGI:95393
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: NNE; Eno-1; MBP-1
Summary: Enables RNA binding activity and phosphopyruvate hydratase activity. Involved in gluconeogenesis and glycolytic process. Acts upstream of or within cellular response to interleukin-7 and in utero embryonic development. Located in cell surface. Is active in cytosol. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in Alzheimer's disease. Orthologous to human ENO1 (enolase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in kidney adult (RPKM 310.6), subcutaneous fat pad adult (RPKM 285.5) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 E2; 4 81.24 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (150321165..150333336)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (150236708..150248879)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Single-cell RNA sequencing unveils unique transcriptomic signatures of endothelial cells and role of ENO1 in response to disturbed flow.
Title: Single-cell RNA sequencing unveils unique transcriptomic signatures of endothelial cells and role of ENO1 in response to disturbed flow.
Unrevealed roles of extracellular enolase-1 (ENO1) in promoting glycolysis and pro-cancer activities in multiple myeloma via hypoxia-inducible factor 1alpha.
Title: Unrevealed roles of extracellular enolase‑1 (ENO1) in promoting glycolysis and pro‑cancer activities in multiple myeloma via hypoxia‑inducible factor 1α.
ENO1 promotes immunosuppression and tumor growth in pancreatic cancer.
Title: ENO1 promotes immunosuppression and tumor growth in pancreatic cancer.
TNF-alpha/ENO1 signaling facilitates testicular phagocytosis by directly activating Elmo1 gene expression in mouse Sertoli cells.
Title: TNF-α/ENO1 signaling facilitates testicular phagocytosis by directly activating Elmo1 gene expression in mouse Sertoli cells.
F. nucleatum targets lncRNA ENO1-IT1 to promote glycolysis and oncogenesis in colorectal cancer.
Title: F. nucleatum targets lncRNA ENO1-IT1 to promote glycolysis and oncogenesis in colorectal cancer.
our data revealed that ENO1 plays a novel and protective role in cerebral ischemia-induced neuronal injury
Title: Enolase1 Alleviates Cerebral Ischemia-Induced Neuronal Injury via Its Enzymatic Product Phosphoenolpyruvate.
regulates the metabolic reprogramming and malignant phenotype of pulmonary artery smooth muscle cells in pulmonary arterial hypertension
Title: Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway.
this paper shows the immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection
Title: The immunoregulatory role of alpha enolase in dendritic cell function during Chlamydia infection.
Eno1 may be a potential marker for low sperm motility induced by iron ion radiation, and these results may provide a useful reference for changes in astronaut fertility during long space missions.
Title: Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice.
The results confirmed the altered expression of HFABP, a key fatty acid transport protein, and of enolase and PGK1, the key enzymes in the glycolytic process
Title: Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI427012 (e-PCR)
- UniSTS:165376

Eno1 (e-PCR), detects polymorphism
- UniSTS:546755

RH125429 (e-PCR)
- UniSTS:162251

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables GTPase binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables heat shock protein binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables phosphopyruvate hydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphopyruvate hydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphopyruvate hydratase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphopyruvate hydratase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables transcription corepressor activity	ISO Inferred from Sequence Orthology more info
enables transcription corepressor binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in canonical glycolysis	ISO Inferred from Sequence Orthology more info
involved_in canonical glycolysis	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within cellular response to acid chemical	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to interleukin-7	IDA Inferred from Direct Assay more info	PubMed
involved_in gluconeogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glyceraldehyde-3-phosphate biosynthetic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycolytic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ATP biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of binding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of muscle contraction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of plasminogen activation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in growth cone	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in myelin sheath	HDA more info	PubMed
located_in nuclear outer membrane	ISO Inferred from Sequence Orthology more info
part_of phosphopyruvate hydratase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphopyruvate hydratase complex	IEA Inferred from Electronic Annotation more info
part_of phosphopyruvate hydratase complex	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in synaptic membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: alpha-enolase

Names: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolase; c-Myc promoter binding protein; non-neural enolase

NP_001366056.1

EC 4.2.1.11

NP_001366057.1

EC 4.2.1.11

NP_075608.2

EC 4.2.1.11

XP_036019540.1

EC 4.2.1.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001379127.2 → NP_001366056.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

AL606903

UniProtKB/TrEMBL

Q6PHC1

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase
NM_001379128.1 → NP_001366057.1 alpha-enolase isoform 2

Status: VALIDATED

Source sequence(s)

AL606903

UniProtKB/Swiss-Prot

P17182, Q99KT7, Q9DCY7

UniProtKB/TrEMBL

Q5FW97, Q5XJG8, Q5XKE1, Q6PHC1

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase
NM_001429235.1 → NP_001416164.1 alpha-enolase isoform 2

Status: VALIDATED

Source sequence(s)

AL606903

UniProtKB/Swiss-Prot

P17182, Q99KT7, Q9DCY7

UniProtKB/TrEMBL

Q5FW97, Q5XJG8, Q5XKE1, Q6PHC1
NM_001429236.1 → NP_001416165.1 alpha-enolase isoform 2

Status: VALIDATED

Source sequence(s)

AL606903

UniProtKB/Swiss-Prot

P17182, Q99KT7, Q9DCY7

UniProtKB/TrEMBL

Q5FW97, Q5XJG8, Q5XKE1, Q6PHC1
NM_001429237.1 → NP_001416166.1 alpha-enolase isoform 2

Status: VALIDATED

Source sequence(s)

AL606903

UniProtKB/Swiss-Prot

P17182, Q99KT7, Q9DCY7

UniProtKB/TrEMBL

Q5FW97, Q5XJG8, Q5XKE1, Q6PHC1
NM_001429238.1 → NP_001416167.1 alpha-enolase isoform 2

Status: VALIDATED

Source sequence(s)

AL606903

UniProtKB/Swiss-Prot

P17182, Q99KT7, Q9DCY7

UniProtKB/TrEMBL

Q5FW97, Q5XJG8, Q5XKE1, Q6PHC1
NM_001429250.1 → NP_001416179.1 alpha-enolase isoform 1

Status: VALIDATED

Source sequence(s)

AL606903

UniProtKB/TrEMBL

Q6PHC1
NM_023119.3 → NP_075608.2 alpha-enolase isoform 2

See identical proteins and their annotated locations for NP_075608.2

Status: VALIDATED

Source sequence(s)

AL606903

Consensus CDS

CCDS18971.1

UniProtKB/Swiss-Prot

P17182, Q99KT7, Q9DCY7

UniProtKB/TrEMBL

Q5FW97, Q5XJG8, Q5XKE1, Q6PHC1

Related

ENSMUSP00000079727.7, ENSMUST00000080926.13

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

150321165..150333336

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036163647.1 → XP_036019540.1 alpha-enolase isoform X1

UniProtKB/TrEMBL

Q6PHC1

Conserved Domains (1) summary

PLN00191
Location:2 → 432

PLN00191; enolase