Top3a topoisomerase (DNA) III alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Top3aprovided by MGI
Official Full Name: topoisomerase (DNA) III alphaprovided by MGI
Primary source: MGI:MGI:1197527
See related: Ensembl:ENSMUSG00000002814 AllianceGenome:MGI:1197527
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Predicted to enable DNA topoisomerase type I (single strand cut, ATP-independent) activity and single-stranded DNA binding activity. Predicted to be involved in DNA metabolic process and chromosome separation. Predicted to be located in PML body. Predicted to be part of RecQ family helicase-topoisomerase III complex. Predicted to be active in nucleus. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; gut; and sensory organ. Human ortholog(s) of this gene implicated in autosomal recessive progressive external ophthalmoplegia with mitochondrial DNA deletions 5. Orthologous to human TOP3A (DNA topoisomerase III alpha). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in testis adult (RPKM 5.5), thymus adult (RPKM 4.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B2; 11 37.81 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (60629014..60668099, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (60738209..60777365, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Top3a (e-PCR), detects polymorphism
- UniSTS:479297

AB006074 (e-PCR)
- UniSTS:160101

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity	ISO Inferred from Sequence Orthology more info
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity	ISS Inferred from Sequence or Structural Similarity more info
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA topological change	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA topological change	ISO Inferred from Sequence Orthology more info
involved_in DNA topological change	ISS Inferred from Sequence or Structural Similarity more info
involved_in chromosome separation	ISO Inferred from Sequence Orthology more info
involved_in chromosome separation	ISS Inferred from Sequence or Structural Similarity more info
involved_in double-strand break repair via homologous recombination	ISO Inferred from Sequence Orthology more info
involved_in mitochondrial DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial DNA metabolic process	ISO Inferred from Sequence Orthology more info
involved_in resolution of DNA recombination intermediates	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in PML body	IEA Inferred from Electronic Annotation more info
located_in PML body	ISO Inferred from Sequence Orthology more info
part_of RecQ family helicase-topoisomerase III complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RecQ family helicase-topoisomerase III complex	ISO Inferred from Sequence Orthology more info
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: DNA topoisomerase 3-alpha

Names: DNA topoisomerase III alpha; Top IIIa; topoisomerase (DNA) 3 alpha; topoisomerase 3 alpha

NP_033436.1

EC 5.6.2.1

XP_036012518.1

EC 5.6.2.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009410.4 → NP_033436.1 DNA topoisomerase 3-alpha

See identical proteins and their annotated locations for NP_033436.1

Status: VALIDATED

Source sequence(s)

AL596215

Consensus CDS

CCDS24797.1

UniProtKB/Swiss-Prot

O70157

UniProtKB/TrEMBL

Q3V3P1, Q5NCT2

Related

ENSMUSP00000002891.5, ENSMUST00000002891.11

Conserved Domains (6) summary

cd03362
Location:35 → 193

TOPRIM_TopoIA_TopoIII; TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving ...

COG0550
Location:35 → 635

TopA; DNA topoisomerase IA [Replication, recombination and repair]

COG0551
Location:652 → 722

YrdD; ssDNA-binding Zn-finger and Zn-ribbon domains of topoisomerase 1 [Replication, recombination and repair]

cd00186
Location:202 → 617

TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...

pfam01396
Location:655 → 694

zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger

pfam06839
Location:897 → 941

zf-GRF; GRF zinc finger

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

60629014..60668099 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156625.1 → XP_036012518.1 DNA topoisomerase 3-alpha isoform X1

UniProtKB/TrEMBL

Q3V3P1

Conserved Domains (4) summary

cd03362
Location:4 → 134

TOPRIM_TopoIA_TopoIII; The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA ...

cd00186
Location:143 → 558

TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...

pfam01396
Location:596 → 635

zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger

pfam06839
Location:838 → 882

zf-GRF; GRF zinc finger