Npas2 neuronal PAS domain protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Npas2provided by MGI
Official Full Name: neuronal PAS domain protein 2provided by MGI
Primary source: MGI:MGI:109232
See related: Ensembl:ENSMUSG00000026077 AllianceGenome:MGI:109232
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MOP4; bHLHe9
Summary: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. The encoded protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq, Dec 2014]
Expression: Broad expression in small intestine adult (RPKM 16.1), colon adult (RPKM 13.4) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 B; 1 17.98 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (39233013..39402321)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (39193702..39363240)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Involvement of Npas2 and Per2 modifications in zinc-induced acute diurnal toxicity in mice.
Title: Involvement of Npas2 and Per2 modifications in zinc-induced acute diurnal toxicity in mice.
Circadian oscillator NPAS2 regulates diurnal expression and activity of CYP1A2 in mouse liver.
Title: Circadian oscillator NPAS2 regulates diurnal expression and activity of CYP1A2 in mouse liver.
A role for the circadian transcription factor NPAS2 in the progressive loss of non-rapid eye movement sleep and increased arousal during fentanyl withdrawal in male mice.
Title: A role for the circadian transcription factor NPAS2 in the progressive loss of non-rapid eye movement sleep and increased arousal during fentanyl withdrawal in male mice.
Circadian transcription factor NPAS2 and the NAD(+) -dependent deacetylase SIRT1 interact in the mouse nucleus accumbens and regulate reward.
Title: Circadian transcription factor NPAS2 and the NAD(+) -dependent deacetylase SIRT1 interact in the mouse nucleus accumbens and regulate reward.
Roles of NPAS2 in circadian rhythm and disease.
Title: Roles of NPAS2 in circadian rhythm and disease.
Circadian-Dependent and Sex-Dependent Increases in Intravenous Cocaine Self-Administration in Npas2 Mutant Mice.
Title: Circadian-Dependent and Sex-Dependent Increases in Intravenous Cocaine Self-Administration in Npas2 Mutant Mice.
Neuronal PAS Domain 2 (Npas2)-Deficient Fibroblasts Accelerate Skin Wound Healing and Dermal Collagen Reconstruction.
Title: Neuronal PAS Domain 2 (Npas2)-Deficient Fibroblasts Accelerate Skin Wound Healing and Dermal Collagen Reconstruction.
Here we have provided an initial key insight into the interplay between neonatal establishment of the peripheral circadian clock in the liver and the ability of the gut microbiome to respond to dietary and metabolic stress.
Title: Conditional postnatal deletion of the neonatal murine hepatic circadian gene, Npas2, alters the gut microbiome following restricted feeding.
In CLOCK-deficient fibroblasts, knockdown of Npas2 leads to arrhythmicity, suggesting that NPAS2 can compensate for loss of CLOCK in peripheral cells as well as in suprachiasmatic nucleus.
Title: NPAS2 Compensates for Loss of CLOCK in Peripheral Circadian Oscillators.
Study identified a unique role for Npas2 in the regulation of cocaine reward and dopamine Drd3 receptor expression
Title: Direct regulation of diurnal Drd3 expression and cocaine reward by NPAS2.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Spontaneous (1) 1 citation
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Npas2 (e-PCR), detects polymorphism
- UniSTS:545970

Npas2 (e-PCR), detects polymorphism
- UniSTS:144052

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables Hsp90 protein binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
involved_in circadian regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in circadian regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in circadian rhythm	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within circadian sleep/wake cycle	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within locomotor rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA repair	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA repair	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of behavioral fear response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to redox state	IDA Inferred from Direct Assay more info	PubMed
involved_in response to redox state	ISO Inferred from Sequence Orthology more info
involved_in response to redox state	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of CLOCK-BMAL transcription complex	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neuronal PAS domain-containing protein 2

Names: neuronal PAS2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008719.2 → NP_032745.2 neuronal PAS domain-containing protein 2

See identical proteins and their annotated locations for NP_032745.2

Status: VALIDATED

Source sequence(s)

BB524729, BC109166, BG070037, U77969

Consensus CDS

CCDS48244.1

UniProtKB/Swiss-Prot

P97460

UniProtKB/TrEMBL

G3X9B7

Related

ENSMUSP00000054719.8, ENSMUST00000056815.9

Conserved Domains (4) summary

TIGR02813
Location:440 → 740

omega_3_PfaA; polyketide-type polyunsaturated fatty acid synthase PfaA

pfam00989
Location:84 → 195

PAS; PAS fold

pfam14598
Location:249 → 352

PAS_11; PAS domain

cd19737
Location:1 → 77

bHLH-PAS_NPAS2_PASD4; basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) domain found in neuronal PAS domain-containing protein 2 (NPAS2) and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

39233013..39402321

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011238452.4 → XP_011236754.1 neuronal PAS domain-containing protein 2 isoform X1

See identical proteins and their annotated locations for XP_011236754.1

UniProtKB/Swiss-Prot

P97460

UniProtKB/TrEMBL

G3X9B7

Conserved Domains (4) summary

TIGR02813
Location:440 → 740

omega_3_PfaA; polyketide-type polyunsaturated fatty acid synthase PfaA

pfam00989
Location:84 → 195

PAS; PAS fold

pfam14598
Location:249 → 352

PAS_11; PAS domain

cd19737
Location:1 → 77

bHLH-PAS_NPAS2_PASD4; basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) domain found in neuronal PAS domain-containing protein 2 (NPAS2) and similar proteins