Plk4 polo like kinase 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Plk4provided by MGI
Official Full Name: polo like kinase 4provided by MGI
Primary source: MGI:MGI:101783
See related: Ensembl:ENSMUSG00000025758 AllianceGenome:MGI:101783
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Sak; Stk18; 1700028H20
Summary: This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to the nucleolus during G2, to centrosomes during G2/M, and to the cleavage furrow during cytokinesis. It is required for progression through mitosis, cell survival, and embryonic development. The mouse genome contains a pseudogene similar to this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Expression: Broad expression in CNS E11.5 (RPKM 11.4), liver E14 (RPKM 9.6) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 B; 3 19.54 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (40754463..40771318)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (40799951..40816883)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition of PLK4 remodels histone methylation and activates the immune response via the cGAS-STING pathway in TP53-mutated AML.
Title: Inhibition of PLK4 remodels histone methylation and activates the immune response via the cGAS-STING pathway in TP53-mutated AML.
Expression Analyses of Polo-Like Kinase 4, a Gene Product Responsible for Autosomal Recessive Microcephaly and Seckel Syndrome, during Mouse Brain Development.
Title: Expression Analyses of Polo-Like Kinase 4, a Gene Product Responsible for Autosomal Recessive Microcephaly and Seckel Syndrome, during Mouse Brain Development.
Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells.
Title: Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells.
Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number.
Title: Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number.
Aurora A and Plk4 are rate-limiting factors contributing to microtubule growth as the acentriolar oocyte resumes meiosis.
Title: Plk4 and Aurora A cooperate in the initiation of acentriolar spindle assembly in mammalian oocytes.
that aneuploidy induced by transient centrosome amplification can accelerate tumorigenesis in p53-deficient cells
Title: Transient PLK4 overexpression accelerates tumorigenesis in p53-deficient epidermis.
Transient knockdown of KLF14 is sufficient to induce Plk4-directed centrosome amplification.
Title: Loss of KLF14 triggers centrosome amplification and tumorigenesis.
PLK4 is essential for meiotic resumption but may not influence spindle formation in mouse oocytes during meiotic maturation
Title: PLK4 is essential for meiotic resumption in mouse oocytes.
p53-Dependent and cell specific epigenetic regulation of the polo-like kinases under oxidative stress.
Title: p53-Dependent and cell specific epigenetic regulation of the polo-like kinases under oxidative stress.
The Plk4-Cep152 complex has an unexpected role in promoting microtubule nucleation in the vicinity of chromosomes to mediate bipolar spindle formation in the absence of centrioles.
Title: Spindle formation in the mouse embryo requires Plk4 in the absence of centrioles.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (7) 1 citation
Gene trapped (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU022933 (e-PCR)
- UniSTS:209422

Stk18 (e-PCR), detects polymorphism
- UniSTS:144519

AI385771 (e-PCR)
- UniSTS:185529

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in centriole replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centriole replication	ISO Inferred from Sequence Orthology more info
involved_in centriole replication	ISS Inferred from Sequence or Structural Similarity more info
involved_in centriole replication	NAS Non-traceable Author Statement more info	PubMed
involved_in cilium assembly	ISO Inferred from Sequence Orthology more info
involved_in cilium assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in de novo centriole assembly involved in multi-ciliated epithelial cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of centriole replication	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of centriole replication	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of centriole replication	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in trophoblast giant cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in XY body	IDA Inferred from Direct Assay more info	PubMed
located_in centriole	ISO Inferred from Sequence Orthology more info
located_in centriole	ISS Inferred from Sequence or Structural Similarity more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in cleavage furrow	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in deuterosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in procentriole	ISO Inferred from Sequence Orthology more info
part_of procentriole replication complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase PLK4

Names: serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine-protein kinase Sak

NP_001397550.1

EC 2.7.11.21

NP_001397551.1

EC 2.7.11.21

NP_035625.2

EC 2.7.11.21

NP_775261.2

EC 2.7.11.21

XP_006500833.1

EC 2.7.11.21

XP_030108378.1

EC 2.7.11.21

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001410621.1 → NP_001397550.1 serine/threonine-protein kinase PLK4 isoform 3

Status: VALIDATED

Source sequence(s)

AC160757

UniProtKB/TrEMBL

E9PVH4
NM_001410622.1 → NP_001397551.1 serine/threonine-protein kinase PLK4 isoform 4

Status: VALIDATED

Source sequence(s)

AC160757

UniProtKB/TrEMBL

E9PVH4
NM_011495.3 → NP_035625.2 serine/threonine-protein kinase PLK4 isoform 1

See identical proteins and their annotated locations for NP_035625.2

Status: VALIDATED

Source sequence(s)

AC160757

Consensus CDS

CCDS17328.1

UniProtKB/Swiss-Prot

Q3UVA3, Q64702, Q6PEP6, Q78EG6, Q80UT6, Q8R0I5, Q9CVR6, Q9CVU6

UniProtKB/TrEMBL

E9PVH4

Related

ENSMUSP00000026858.6, ENSMUST00000026858.11

Conserved Domains (5) summary

smart00220
Location:12 → 265

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd13114
Location:548 → 659

POLO_box_Plk4_1; First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13115
Location:660 → 768

POLO_box_Plk4_2; Second (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13116
Location:839 → 918

POLO_box_Plk4_3; C-terminal (third) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd14186
Location:10 → 265

STKc_PLK4; Catalytic domain of the Serine/Threonine Kinase, Polo-like kinase 4
NM_173169.3 → NP_775261.2 serine/threonine-protein kinase PLK4 isoform 2

Status: VALIDATED

Source sequence(s)

AC160757

Consensus CDS

CCDS57210.1

UniProtKB/TrEMBL

E9PVH4

Related

ENSMUSP00000145277.2, ENSMUST00000203295.3

Conserved Domains (5) summary

smart00220
Location:12 → 265

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd13114
Location:548 → 632

POLO_box_Plk4_1; First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13115
Location:633 → 741

POLO_box_Plk4_2; Second (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13116
Location:812 → 891

POLO_box_Plk4_3; C-terminal (third) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd14186
Location:10 → 265

STKc_PLK4; Catalytic domain of the Serine/Threonine Kinase, Polo-like kinase 4

RNA

NR_045576.2 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC160757

Related

ENSMUST00000203895.3

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

40754463..40771318

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006500770.5 → XP_006500833.1 serine/threonine-protein kinase PLK4 isoform X2

Conserved Domains (3) summary

cd13114
Location:122 → 233

POLO_box_Plk4_1; First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13115
Location:234 → 342

POLO_box_Plk4_2; Second (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13116
Location:413 → 492

POLO_box_Plk4_3; C-terminal (third) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)
XM_030252518.2 → XP_030108378.1 serine/threonine-protein kinase PLK4 isoform X2

Conserved Domains (3) summary

cd13114
Location:122 → 233

POLO_box_Plk4_1; First (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13115
Location:234 → 342

POLO_box_Plk4_2; Second (cryptic) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)

cd13116
Location:413 → 492

POLO_box_Plk4_3; C-terminal (third) polo-box domain (PBD) of polo-like kinase 4 (Plk4/Sak)