Chrnb4 cholinergic receptor, nicotinic, beta polypeptide 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrnb4provided by MGI
Official Full Name: cholinergic receptor, nicotinic, beta polypeptide 4provided by MGI
Primary source: MGI:MGI:87892
See related: Ensembl:ENSMUSG00000035200 AllianceGenome:MGI:87892
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Acrb4; Acrb-4
Summary: Enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential. Involved in positive regulation of transmission of nerve impulse. Acts upstream of or within several processes, including behavioral response to nicotine; neuronal action potential; and synaptic transmission involved in micturition. Is active in cholinergic synapse and postsynaptic specialization membrane. Is expressed in several structures, including alimentary system; eye; genitourinary system; nervous system; and thymus. Human ortholog(s) of this gene implicated in cocaine abuse; lung cancer (multiple); nicotine dependence; and oral cavity cancer. Orthologous to human CHRNB4 (cholinergic receptor nicotinic beta 4 subunit). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in adrenal adult (RPKM 3.4), colon adult (RPKM 3.2) and 15 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 B; 9 29.84 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (54935438..54956236, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (55028154..55048953, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Abundant extrasynaptic expression of alpha3beta4-containing nicotinic acetylcholine receptors in the medial habenula-interpeduncular nucleus pathway in mice.
Title: Abundant extrasynaptic expression of α3β4-containing nicotinic acetylcholine receptors in the medial habenula-interpeduncular nucleus pathway in mice.
Differential tyrosine and serine/threonine phosphorylation/dephosphorylation pathways regulate the expression of alpha7 versus alpha3beta4 nicotinic receptor subtypes in mouse hippocampal neurons.
Title: Differential tyrosine and serine/threonine phosphorylation/dephosphorylation pathways regulate the expression of α7 versus α3β4 nicotinic receptor subtypes in mouse hippocampal neurons.
The majority of nicotinic receptors at the cell surface of superior cervical ganglia neurons consist of alpha3beta4alpha5 subunits.
Title: The role of the nAChR subunits α5, β2, and β4 on synaptic transmission in the mouse superior cervical ganglion.
These findings suggest that beta4* nAChRs in the interpeduncular nucleus have a role in maintaining nicotine intravenous self-administration.
Title: Role of β4* Nicotinic Acetylcholine Receptors in the Habenulo-Interpeduncular Pathway in Nicotine Reinforcement in Mice.
Data suggest that the nicotinic acetylcholine receptor beta4 subunit (beta4 subunit) is not involved in ethanol responses.
Title: No evidence of a role of the β4 subunit of the nicotinic acetylcholine receptor in alcohol-related behaviors.
MAFA controls autonomic nervous system-mediated insulin secretion by activating the transcription of nicotinic (ChrnB2 and ChrnB4) receptor genes, which is impaired in type 2 diabetes.
Title: MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes.
The alpha3beta4* nicotinic ACh receptor subtype mediates physical dependence to morphine
Title: The α3β4* nicotinic ACh receptor subtype mediates physical dependence to morphine: mouse and human studies.
nAChRs that contain the beta4 subunit are important for social behaviors.
Title: Abnormal social behavior in nicotinic acetylcholine receptor β4 subunit-null mice.
Downregulation of nAChR subunit and PSD-93 expression after cavernous nerve injury, or even manipulation, could interrupt synaptic transmission within the MPG and thus contribute to the loss of neural control of urogenital organs after pelvic surgeries.
Title: Decrease in neuronal nicotinic acetylcholine receptor subunit and PSD-93 transcript levels in the male mouse MPG after cavernous nerve injury or explant culture.
findings suggest an important role for the a3b4* nAChR subtype in nicotine reward and physical aspects of the nicotine withdrawal syndrome.
Title: The α3β4* nicotinic acetylcholine receptor subtype mediates nicotine reward and physical nicotine withdrawal signs independently of the α5 subunit in the mouse.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (2) 1 citation

General gene information

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Markers

D8Wox29 (e-PCR)
- UniSTS:239876

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

Chrnb4 (e-PCR), detects polymorphism
- UniSTS:256428

Chrnb4 (e-PCR), detects polymorphism
- UniSTS:506800

D9Ucl1 (e-PCR), detects polymorphism
- UniSTS:142813

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acetylcholine binding	IEA Inferred from Electronic Annotation more info
enables acetylcholine binding	ISO Inferred from Sequence Orthology more info
enables acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine receptor activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine-gated monoatomic cation-selective channel activity	ISO Inferred from Sequence Orthology more info
enables heterocyclic compound binding	IEA Inferred from Electronic Annotation more info
contributes_to heterocyclic compound binding	ISO Inferred from Sequence Orthology more info
enables heterocyclic compound binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within behavioral response to nicotine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuronal action potential	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transmission of nerve impulse	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of membrane potential	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to nicotine	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within smooth muscle contraction	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within synaptic transmission involved in micturition	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within synaptic transmission involved in micturition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic transmission involved in micturition	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission, cholinergic	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IEA Inferred from Electronic Annotation more info
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
is_active_in cholinergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in cholinergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in postsynaptic specialization membrane	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neuronal acetylcholine receptor subunit beta-4

Names: nicotinic acetylcholine receptor beta4 subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_148944.4 → NP_683746.1 neuronal acetylcholine receptor subunit beta-4 precursor

See identical proteins and their annotated locations for NP_683746.1

Status: PROVISIONAL

Source sequence(s)

AC156832

Consensus CDS

CCDS23200.1

UniProtKB/Swiss-Prot

Q8R493, Q8VI06

UniProtKB/TrEMBL

Q3USV2

Related

ENSMUSP00000034854.7, ENSMUST00000034854.8

Conserved Domains (3) summary

TIGR00860
Location:21 → 483

LIC; Cation transporter family protein

cd03558
Location:152 → 166

LGIC_ECD; Cys-loop [structural motif]

cl28912
Location:53 → 230

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

54935438..54956236 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011242655.4 → XP_011240957.1 neuronal acetylcholine receptor subunit beta-4 isoform X1

See identical proteins and their annotated locations for XP_011240957.1

Conserved Domains (3) summary

TIGR00860
Location:2 → 454

LIC; Cation transporter family protein

cd03558
Location:123 → 137

LGIC_ECD; Cys-loop [structural motif]

cl28912
Location:24 → 201

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))
XM_006510757.5 → XP_006510820.1 neuronal acetylcholine receptor subunit beta-4 isoform X1

See identical proteins and their annotated locations for XP_006510820.1

Conserved Domains (3) summary

TIGR00860
Location:2 → 454

LIC; Cation transporter family protein

cd03558
Location:123 → 137

LGIC_ECD; Cys-loop [structural motif]

cl28912
Location:24 → 201

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))