Mixl1 Mix paired-like homeobox [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mixl1provided by MGI
Official Full Name: Mix paired-like homeoboxprovided by MGI
Primary source: MGI:MGI:1351322
See related: Ensembl:ENSMUSG00000026497 AllianceGenome:MGI:1351322
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mm1; Mml
Summary: Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and protein homodimerization activity. Involved in several processes, including endodermal cell differentiation; negative regulation of hematopoietic progenitor cell differentiation; and positive regulation of mesoderm development. Acts upstream of or within several processes, including digestive tract development; gastrulation; and hemopoiesis. Located in chromatin and nucleus. Is expressed in several structures, including early embryo; egg cylinder; embryo mesoderm; metanephros; and primitive streak. Orthologous to human MIXL1 (Mix paired-like homeobox). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Low expression observed in reference dataset See more
Orthologs: human all
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Genomic context

Location:: 1 H4; 1 84.46 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (180520608..180524599, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (180693043..180697034, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In this study, we demonstrated that forced expression of Mix-like protein 1 (encoded by Mixl1) can be used to guide contribution of mouse embryonic stem cells to endodermal organs after blastocyst injection.
Title: Targeted organ generation using Mixl1-inducible mouse pluripotent stem cells in blastocyst complementation.
Embryonic localization of Mixl1 shows its co-localized with the early hematopoietic marker, Runx1, in the allantois and visceral yolk sac blood islands.
Title: Mixl1 localizes to putative axial stem cell reservoirs and their posterior descendants in the mouse embryo.
At later stages, in contrast with wild-type embryos, E7.5-8.0, Mixl1 knockout (KO) KO/KO embryos lacked morphologically recognizable midline structures such as the node and notochord.
Title: A conditional mutant allele for analysis of Mixl1 function in the mouse.
Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity
Title: Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity.
These data support the hypothesis that Mixl1 directly regulates Pdgfralpha and Flk1 gene expression.
Title: Pdgfrα and Flk1 are direct target genes of Mixl1 in differentiating embryonic stem cells.
results strongly suggest that Gsc is a direct target gene of Mixl1 during embryogenesis.
Title: Transcriptional activation by the Mixl1 homeodomain protein in differentiating mouse embryonic stem cells.
The studies suggest that high levels of Mixl1 preferentially allocate cells to the endoderm during ESC differentiation.
Title: Enforced expression of Mixl1 during mouse ES cell differentiation suppresses hematopoietic mesoderm and promotes endoderm formation.
Overexpression of Mixl1 appears capable of inducing an abnormal self-renewal capacity in granulocytic precursors
Title: The preleukemic state of mice reconstituted with Mixl1-transduced marrow cells.
As goosecoid is itself induced in a Foxh1-dependent manner, we propose that Foxh1 initiates positive and negative transcriptional circuits to refine cell fate decisions during gastrulation
Title: Foxh1 recruits Gsc to negatively regulate Mixl1 expression during early mouse development.
non-cell autonomous role in nascent mesoderm in the formation and/or patterning of adjacent definitive endoderm.
Title: Mouse Mix gene is activated early during differentiation of ES and F9 stem cells and induces endoderm in frog embryos.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:234289

Mixl1 (e-PCR), detects polymorphism
- UniSTS:547263

NoName (e-PCR)
- UniSTS:489193

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell migration involved in gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within digestive tract development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endoderm development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoderm formation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within endoderm formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endodermal cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hematopoietic progenitor cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within hemopoiesis	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of hematopoietic progenitor cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of mesoderm development	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of mesoderm development	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: homeobox protein MIXL1

Names: MIX1 homeobox-like protein 1; Mix1 homeobox-like 1; homeodomain protein MIX; mMix; mix.1 homeobox-like protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.