WLS Wnt ligand secretion mediator [Homo sapiens (human)] - Gene

Summary

Official Symbol: WLSprovided by HGNC
Official Full Name: Wnt ligand secretion mediatorprovided by HGNC
Primary source: HGNC:HGNC:30238
See related: Ensembl:ENSG00000116729 MIM:611514; AllianceGenome:HGNC:30238
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: EVI; MRP; ZKS; GPR177; mig-14; C1orf139
Summary: Enables Wnt-protein binding activity and identical protein binding activity. Involved in positive regulation of cell communication and protein transport. Located in several cellular components, including Golgi apparatus; early endosome; and endoplasmic reticulum membrane. Implicated in Zaki syndrome. [provided by Alliance of Genome Resources, Jan 2025]
Expression: Ubiquitous expression in kidney (RPKM 32.7), gall bladder (RPKM 31.7) and 24 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1p31.3

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (68098459..68232546, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (67975934..68110470, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (68564142..68698229, complement)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Tissue-specific circular RNA induction during human fetal development
Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
BioProject: PRJNA270632
Publication: PMID 26076956
Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Expanding the phenotypic spectrum and clinical severity associated with WLS gene.
Title: Expanding the phenotypic spectrum and clinical severity associated with WLS gene.
Compound heterozygous variants in WLS gene causes Zaki syndrome.
Title: Compound heterozygous variants in WLS gene causes Zaki syndrome.
Mutations in the WLS are associated with dental anomalies, torus palatinus, and torus mandibularis.
Title: Mutations in the WLS are associated with dental anomalies, torus palatinus, and torus mandibularis.
The role of Evi/Wntless in exporting Wnt proteins.
Title: The role of Evi/Wntless in exporting Wnt proteins.
GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain via WNT-mediated TRPV1 activation.
Title: GPR177 in A-fiber sensory neurons drives diabetic neuropathic pain via WNT-mediated TRPV1 activation.
Responses of Porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses.
Title: Responses of Porcupine and Wntless proteins to oxidative, hypoxic and endoplasmic reticulum stresses.
Structural Basis of WLS/Evi-Mediated Wnt Transport and Secretion.
Title: Structural Basis of WLS/Evi-Mediated Wnt Transport and Secretion.
Cryo-EM structure of human Wntless in complex with Wnt3a.
Title: Cryo-EM structure of human Wntless in complex with Wnt3a.
SNHG17 upregulates WLS expression to accelerate lung adenocarcinoma progression by sponging miR-485-5p.
Title: SNHG17 upregulates WLS expression to accelerate lung adenocarcinoma progression by sponging miR-485-5p.
MicroRNA-31 Regulates Expression of Wntless in Both Drosophila melanogaster and Human Oral Cancer Cells.
Title: MicroRNA-31 Regulates Expression of Wntless in Both Drosophila melanogaster and Human Oral Cancer Cells.

Submit: New GeneRIF Correction See all GeneRIFs (36)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Zaki syndrome MedGen: C5562037 OMIM: 619648 GeneReviews: Not available	not available

EBI GWAS Catalog

Description
Genome-wide association studies identify CHRNA5/3 and HTR4 in the development of airflow obstruction. EBI GWAS Catalog EBI GWAS CatalogPubMed
Genome-wide association study of chemotherapeutic agent-induced severe neutropenia/leucopenia for patients in Biobank Japan. EBI GWAS Catalog EBI GWAS CatalogPubMed
Genome-wide meta-analysis identifies 56 bone mineral density loci and reveals 14 loci associated with risk of fracture. EBI GWAS Catalog EBI GWAS CatalogPubMed
Multistage genome-wide association meta-analyses identified two new loci for bone mineral density. EBI GWAS Catalog EBI GWAS CatalogPubMed
Twenty bone-mineral-density loci identified by large-scale meta-analysis of genome-wide association studies. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH68355 (e-PCR)
- UniSTS:70150

RH64518 (e-PCR)
- UniSTS:36171

G09848 (e-PCR)
- UniSTS:3433

A004O04 (e-PCR)
- UniSTS:5739

A006M39 (e-PCR)
- UniSTS:44160

D1S1583 (e-PCR)
- UniSTS:45976

G60301 (e-PCR)
- UniSTS:137406

G20702 (e-PCR)
- UniSTS:44734

A006C26 (e-PCR)
- UniSTS:44735

RH92980 (e-PCR)
- UniSTS:91593

HSC2OB072 (e-PCR)
- UniSTS:67291

RH122841 (e-PCR)
- UniSTS:135139

Clone Names

FLJ23091, MGC14878, MGC131760, DKFZp686I0788

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables Wnt-protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables Wnt-protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables Wnt-protein binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Wnt protein secretion	IBA Inferred from Biological aspect of Ancestor more info
involved_in Wnt protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in anterior/posterior axis specification	ISS Inferred from Sequence or Structural Similarity more info
involved_in cementum mineralization	IEA Inferred from Electronic Annotation more info
involved_in exocrine pancreas development	IEA Inferred from Electronic Annotation more info
involved_in hindbrain development	IEA Inferred from Electronic Annotation more info
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mesoderm formation	IEA Inferred from Electronic Annotation more info
involved_in midbrain development	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of Wnt protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Wnt protein secretion	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	TAS Traceable Author Statement more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome membrane	TAS Traceable Author Statement more info
located_in endocytic vesicle membrane	TAS Traceable Author Statement more info
is_active_in endomembrane system	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	TAS Traceable Author Statement more info
is_active_in organelle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	TAS Traceable Author Statement more info
located_in trans-Golgi network	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein wntless homolog

Names: G protein-coupled receptor 177; integral membrane protein GPR177; protein evenness interrupted homolog; putative NF-kappa-B-activating protein 373; putative NFkB activating protein 373; wntless Wnt ligand secretion mediator

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001002292.4 → NP_001002292.3 protein wntless homolog isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, and differs in the 5' UTR, 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is longer than isoform 1.

Source sequence(s)

AL513284, BX538320, CA438784, DA753300

Consensus CDS

CCDS30750.1

UniProtKB/TrEMBL

Q96IV8

Related

ENSP00000346829.2, ENST00000354777.6

Conserved Domains (1) summary

pfam06664
Location:176 → 494

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion
NM_001193334.1 → NP_001180263.1 protein wntless homolog isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1.

Source sequence(s)

AL513284, AW137622, BX648748, DA753300, DB478499

Consensus CDS

CCDS53331.1

UniProtKB/TrEMBL

Q96IV8

Related

ENSP00000360015.3, ENST00000370976.7

Conserved Domains (1) summary

pfam06664
Location:87 → 405

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion
NM_024911.7 → NP_079187.3 protein wntless homolog isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.

Source sequence(s)

AW137622, DQ323735

Consensus CDS

CCDS642.1

UniProtKB/Swiss-Prot

B2RNT2, Q5JRS7, Q5T9L3, Q7Z2Z9, Q8NC43

UniProtKB/TrEMBL

Q96IV8

Related

ENSP00000262348.4, ENST00000262348.9

Conserved Domains (1) summary

pfam06664
Location:178 → 496

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000001.11 Reference GRCh38.p14 Primary Assembly

Range

68098459..68232546 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011542191.3 → XP_011540493.1 protein wntless homolog isoform X1

UniProtKB/TrEMBL

Q96IV8

Conserved Domains (1) summary

pfam06664
Location:178 → 496

MIG-14_Wnt-bd; Wnt-binding factor required for Wnt secretion

Alternate T2T-CHM13v2.0

Genomic

NC_060925.1 Alternate T2T-CHM13v2.0

Range

67975934..68110470 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054338846.1 → XP_054194821.1 protein wntless homolog isoform X1

UniProtKB/TrEMBL

Q96IV8
XM_054338847.1 → XP_054194822.1 protein wntless homolog isoform X2

UniProtKB/TrEMBL

Q96IV8