ppp1r12a protein phosphatase 1, regulatory subunit 12A [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: ppp1r12aprovided by ZNC
Official Full Name: protein phosphatase 1, regulatory subunit 12Aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-041011-3
See related: Ensembl:ENSDARG00000010784 AllianceGenome:ZFIN:ZDB-GENE-041011-3
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: zgc:110448; unm_p82emcf; si:dkey-28j4.1
Summary: Enables protein heterodimerization activity. Acts upstream of or within several processes, including actin filament bundle organization; convergent extension involved in axis elongation; and nervous system development. Located in cytoplasm. Is expressed in several structures, including central nervous system; digestive system; endoderm; mesoderm; and myotome. Orthologous to several human genes including PPP1R12A (protein phosphatase 1 regulatory subunit 12A). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 4

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	4	NC_007115.7 (21488990..21584057, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	4	NC_007115.6 (21768015..21863103, complement)

Chromosome 4 - NC_007115.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mypt1 regulates Bmp signaling to promote embryonic exocrine pancreas growth in zebrafish.
Title: Mypt1 regulates Bmp signaling to promote embryonic exocrine pancreas growth in zebrafish.
The findings define the PI3K/NUAK1/MYPT1/MLCP axis as a critical pathway to regulate actomyosin contractility in endothelial cells, supporting vascular patterning and expansion through the control of cell rearrangement.
Title: Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility.
Mypt1-202 plays an important role in maintaining baseline Mlc2 dephosphorylation and actomyosin relaxation during early zebrafish development.
Title: Alternative splicing of (ppp1r12a/mypt1) in zebrafish produces a novel myosin phosphatase targeting subunit.
zebrafish have two genes encoding PP1beta, both of which can interact with Mypt1.
Title: Protein phosphatase 1 β paralogs encode the zebrafish myosin phosphatase catalytic subunit.
e find that this alignment requires myosin phosphatase activity within motoneurons, and that mutations in the myosin phosphatase subunit mypt1 increase myosin phosphorylation causing a displacement between motoneuron cell bodies and their axons.
Title: Myosin phosphatase Fine-tunes Zebrafish Motoneuron Position during Axonogenesis.
Mypt1 mediates coordination between mesoderm and endoderm cell movements in order to carefully position the liver primordium such that it receives a Bmp2 signal that is essential for liver formation (Mypt1)
Title: Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis.
Epithelial relaxation mediated by the myosin phosphatase regulator Mypt1 is required for brain ventricle lumen expansion and hindbrain morphogenesis.
Title: Epithelial relaxation mediated by the myosin phosphatase regulator Mypt1 is required for brain ventricle lumen expansion and hindbrain morphogenesis.

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General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables 14-3-3 protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables enzyme inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables myosin phosphatase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatase regulator activity	IEA Inferred from Electronic Annotation more info
enables phosphatase regulator activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein heterodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin filament bundle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within actin filament bundle distribution	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within convergent extension involved in axis elongation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of exocrine pancreas development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within liver development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within midbrain-hindbrain boundary morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within motor neuron axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of BMP signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell adhesion	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in A band	IBA Inferred from Biological aspect of Ancestor more info
located_in A band	ISS Inferred from Sequence or Structural Similarity more info
part_of PTW/PP1 phosphatase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
located_in Z disc	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein phosphatase 1 regulatory subunit 12A

Names: myosin phosphatase target subunit 1; myosin phosphatase-targeting subunit 1; myosin-binding subunit of myosin phosphatase; mypt1; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit; unm p82emcf

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001003870.2 → NP_001003870.2 protein phosphatase 1 regulatory subunit 12A

Status: VALIDATED

Source sequence(s)

BX537286

UniProtKB/Swiss-Prot

Q4G0B0, Q5TZ13, Q6DRG7

Related

ENSDARP00000118021.1, ENSDART00000132923.3

Conserved Domains (4) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:47 → 136

Ank_2; Ankyrin repeats (3 copies)

pfam15898
Location:950 → 1048

PRKG1_interact; cGMP-dependent protein kinase interacting domain

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007115.7 Reference GRCz11 Primary Assembly

Range

21488990..21584057 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_021474999.2 → XP_021330674.1 protein phosphatase 1 regulatory subunit 12A isoform X6

UniProtKB/TrEMBL

A0A8M9Q0E6

Conserved Domains (4) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:902 → 1000

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cl26073
Location:47 → 312

Ank_2; Ankyrin repeats (3 copies)
XM_017354997.3 → XP_017210486.1 protein phosphatase 1 regulatory subunit 12A isoform X3

UniProtKB/TrEMBL

A0A8M6YX05
XM_021474997.2 → XP_021330672.1 protein phosphatase 1 regulatory subunit 12A isoform X4

UniProtKB/TrEMBL

A0A8M9PH66

Conserved Domains (4) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:950 → 1054

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cl26073
Location:47 → 312

Ank_2; Ankyrin repeats (3 copies)
XM_021474998.2 → XP_021330673.1 protein phosphatase 1 regulatory subunit 12A isoform X5

UniProtKB/TrEMBL

A0A8M9PUD2

Conserved Domains (4) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:934 → 1038

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cl26073
Location:47 → 312

Ank_2; Ankyrin repeats (3 copies)
XM_021474996.2 → XP_021330671.1 protein phosphatase 1 regulatory subunit 12A isoform X2

UniProtKB/TrEMBL

A0A8M9Q5V2

Conserved Domains (4) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:975 → 1079

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cl26073
Location:47 → 312

Ank_2; Ankyrin repeats (3 copies)
XM_021474995.2 → XP_021330670.1 protein phosphatase 1 regulatory subunit 12A isoform X1

UniProtKB/TrEMBL

A0A8M9QB53

Conserved Domains (4) summary

cd00204
Location:67 → 252

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

sd00045
Location:72 → 103

ANK; ANK repeat [structural motif]

pfam15898
Location:982 → 1086

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cl26073
Location:47 → 312

Ank_2; Ankyrin repeats (3 copies)