arl-8 ADP-ribosylation factor-like protein 8B [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: arl-8
Official Full Name: ADP-ribosylation factor-like protein 8B
Primary source: WormBase:WBGene00000192
Locus tag: CELE_Y57G11C.13
See related: AllianceGenome:WB:WBGene00000192
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable GTP binding activity and GTPase activity. Involved in axo-dendritic transport; endosome to lysosome transport; and regulation of anterograde synaptic vesicle transport. Located in lysosome; presynaptic active zone; and synaptic vesicle. Is expressed in several structures, including coelomocyte; intestine; nervous system; pharynx; and reproductive system. Orthologous to several human genes including ARL8A (ARF like GTPase 8A). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See arl-8 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 3

Sequence:: Chromosome: IV; NC_003282.8 (14798149..14799227)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our results indicate that an ARF-like GTPase gene product, ARL-8, mitigates endocytic Ab neurodegeneration in a VPS-41-dependent manner, rather than through RAB-7 and AP3 as with alpha-synuclein..we demonstrate that the LC3 orthologue, LGG-2, plays a critical role in Ab toxicity with ARL-8.
Title: Distinct functional roles of Vps41-mediated neuroprotection in Alzheimer's and Parkinson's disease models of neurodegeneration.
Synaptic vesicle bound small GTPase ARL-8 activates UNC-104 by unlocking the autoinhibition. The results demonstrate that the autoinhibitory mechanism is utilized to regulate the distribution of transport cargoes and is important for synaptogenesis in vivo.
Title: Autoinhibition of a Neuronal Kinesin UNC-104/KIF1A Regulates the Size and Density of Synapses.
ARL-8 mediates delivery of endocytosed macromolecules to lysosomes by facilitating late endosome-lysosome fusion.
Title: The arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans.
ARL-8 facilitates apoptotic cell removal in vivo by mediating phagosome-lysosome fusion during phagocytosis.
Title: Arl8/ARL-8 functions in apoptotic cell removal by mediating phagolysosome formation in Caenorhabditis elegans.
ARL-8 inhibits presynaptic assembly by promoting dissociation.
Title: The balance between capture and dissociation of presynaptic proteins controls the spatial distribution of synapses.
ARL-8 is required during presynaptic assembly of requisite proteins into vesicular cargoes in the cell soma, their long-distance microtubule-dependent transport down the axon, and their reconstitution into functional complexes at prespecified sites.
Title: An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y57G11C.13 (e-PCR)
- UniSTS:316221

Y57G11C.12 (e-PCR)
- UniSTS:321094

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in anterograde axonal transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in axo-dendritic transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axo-dendritic transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosome to lysosome transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of anterograde synaptic vesicle transport	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of anterograde synaptic vesicle transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in lysosomal membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosomal membrane	IEA Inferred from Electronic Annotation more info
located_in lysosome	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IEA Inferred from Electronic Annotation more info
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ADP-ribosylation factor-like protein 8B

NP_502791.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

14798149..14799227

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_070390.10 → NP_502791.1 ADP-ribosylation factor-like protein 8B [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_502791.1

Status: REVIEWED

UniProtKB/TrEMBL

G5EDC6

Conserved Domains (2) summary

cd04159
Location:22 → 180

Arl10_like; Arf-like 9 (Arl9) and 10 (Arl10) GTPases

pfam00071
Location:23 → 178

Ras; Ras family