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    Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 [ Mus musculus (house mouse) ]

    Gene ID: 170768, updated on 14-Dec-2024

    Summary

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    Official Symbol
    Pfkfb3provided by MGI
    Official Full Name
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3provided by MGI
    Primary source
    MGI:MGI:2181202
    See related
    Ensembl:ENSMUSG00000026773 AllianceGenome:MGI:2181202
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    iPFK-2; uPFK-2; E330010H22Rik
    Summary
    Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Acts upstream of or within several processes, including glial cell differentiation; glycolytic process; and motor behavior. Predicted to be located in nucleoplasm. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; integumental system; nervous system; sensory organ; and skeleton. Used to study extrinsic cardiomyopathy. Orthologous to human PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Broad expression in subcutaneous fat pad adult (RPKM 57.0), genital fat pad adult (RPKM 44.9) and 22 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Pfkfb3 in Genome Data Viewer
    Location:
    2 A1; 2 8.73 cM
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (11476241..11558882, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (11471430..11554071, complement)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene predicted gene, 31990 Neighboring gene STARR-positive B cell enhancer ABC_E5882 Neighboring gene STARR-seq mESC enhancer starr_03667 Neighboring gene predicted gene, 52500 Neighboring gene predicted gene 10851 Neighboring gene STARR-seq mESC enhancer starr_03668 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:11470208-11470361 Neighboring gene STARR-positive B cell enhancer mm9_chr2:11475523-11475823 Neighboring gene STARR-seq mESC enhancer starr_03673 Neighboring gene STARR-positive B cell enhancer ABC_E5883 Neighboring gene RNA binding motif protein 17 Neighboring gene proteasome (prosome, macropain) 26S subunit, ATPase, 6 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. A role for inducible 6-phosphofructo-2-kinase in the control of neuronal glycolysis. Li H, et al. J Nutr Biochem, 2013 Jun. PMID 23246158, Free PMC Article
    2. IRF8 and BATF3 interaction enhances the cDC1 specific Pfkfb3 gene expression. Chauhan KS, et al. Cell Immunol, 2022 Jan. PMID 34968772
    3. Disruption of endothelial Pfkfb3 ameliorates diet-induced murine insulin resistance. Yang Q, et al. J Endocrinol, 2021 Jul 14. PMID 34101614
    4. Gene-dosage effect of Pfkfb3 on monocyte/macrophage biology in atherosclerosis. Guo S, et al. Br J Pharmacol, 2022 Nov. PMID 35834356, Free PMC Article
    5. Differential role of neuronal glucose and PFKFB3 in memory formation during development. Cruz E, et al. Glia, 2022 Nov. PMID 35916383, Free PMC Article

    See all (106) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. PFKFB3 ameliorates ischemia-induced neuronal damage by reducing reactive oxygen species and inhibiting nuclear translocation of Cdk5.
      Title: PFKFB3 ameliorates ischemia-induced neuronal damage by reducing reactive oxygen species and inhibiting nuclear translocation of Cdk5.
    2. The glycolytic enzyme PFKFB3 drives kidney fibrosis through promoting histone lactylation-mediated NF-kappaB family activation.
      Title: The glycolytic enzyme PFKFB3 drives kidney fibrosis through promoting histone lactylation-mediated NF-κB family activation.
    3. Hypoxia activates macrophage-NLRP3 inflammasome promoting atherosclerosis via PFKFB3-driven glycolysis.
      Title: Hypoxia activates macrophage-NLRP3 inflammasome promoting atherosclerosis via PFKFB3-driven glycolysis.
    4. MiR-106a-5p targets PFKFB3 and improves sepsis through regulating macrophage pyroptosis and inflammatory response.
      Title: MiR-106a-5p targets PFKFB3 and improves sepsis through regulating macrophage pyroptosis and inflammatory response.
    5. Expression of Pfkfb isoenzymes during in vitro differentiation of mouse embryonic stem cells into insulin-producing cells.
      Title: Expression of Pfkfb isoenzymes during in vitro differentiation of mouse embryonic stem cells into insulin-producing cells.
    6. Cardiac-specific PFKFB3 overexpression prevents diabetic cardiomyopathy via enhancing OPA1 stabilization mediated by K6-linked ubiquitination.
      Title: Cardiac-specific PFKFB3 overexpression prevents diabetic cardiomyopathy via enhancing OPA1 stabilization mediated by K6-linked ubiquitination.
    7. PFKFB3-mediated glycolysis in hepatic stellate cells promotes liver regeneration.
      Title: PFKFB3-mediated glycolysis in hepatic stellate cells promotes liver regeneration.
    8. Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis.
      Title: Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis.
    9. IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1alpha/PFKFB3 pathway.
      Title: IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1α/PFKFB3 pathway.
    10. PFKFB3-Meditated Glycolysis via the Reactive Oxygen Species-Hypoxic Inducible Factor 1alpha Axis Contributes to Inflammation and Proliferation of Staphylococcus aureus in Epithelial Cells.
      Title: PFKFB3-Meditated Glycolysis via the Reactive Oxygen Species-Hypoxic Inducible Factor 1α Axis Contributes to Inflammation and Proliferation of Staphylococcus aureus in Epithelial Cells.
    Submit: New GeneRIF Correction See all GeneRIFs (60)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 6-phosphofructo-2-kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 6-phosphofructo-2-kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in fructose 2,6-bisphosphate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in fructose 2,6-bisphosphate metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within glial cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within glycolytic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within motor behavior IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
    Names
    inducible 6-phosphofructo-2-kinase
    NP_001171223.1
    NP_001171224.1
    NP_001171225.1
    NP_001171226.1
    NP_001171227.1
    NP_001171228.1
    NP_001171229.1
    NP_573495.2
    XP_006497430.1
    XP_006497432.1
    XP_017171443.1
    XP_030103781.1
    XP_030103790.1
    XP_036014832.1

    NCBI Reference Sequences (RefSeq)

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    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001177752.1 → NP_001171223.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 1

      See identical proteins and their annotated locations for NP_001171223.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) lacks 4 exons in the 3' coding region, compared to variant 2. The resulting isoform (1) has a shorter and distinct C-terminus, compared to isoform 2.
      Source sequence(s)
      BB553577, BC052400, BP764506, BY267778, EU034675
      Consensus CDS
      CCDS50498.1
      UniProtKB/TrEMBL
      A2AUP3, A7UAK4, V9GWY6
      Related
      ENSMUSP00000138893.3, ENSMUST00000183869.8
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    2. NM_001177753.1 → NP_001171224.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2

      See identical proteins and their annotated locations for NP_001171224.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).
      Source sequence(s)
      BB553577, BC052400, BP764506, BY267778, EU034676
      Consensus CDS
      CCDS50501.1
      UniProtKB/TrEMBL
      A2AUP4, A7UAK5
      Related
      ENSMUSP00000142079.2, ENSMUST00000191668.6
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    3. NM_001177754.1 → NP_001171225.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 3

      See identical proteins and their annotated locations for NP_001171225.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) lacks two alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (3) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 2.
      Source sequence(s)
      BB553577, BC052400, BP764506, BY267778, EU034677
      Consensus CDS
      CCDS79731.1
      UniProtKB/TrEMBL
      A2AUP3, A2AUP5, A7UAK6
      Related
      ENSMUSP00000050926.8, ENSMUST00000049849.12
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    4. NM_001177755.1 → NP_001171226.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 4

      See identical proteins and their annotated locations for NP_001171226.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) lacks two alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (4) has a shorter and distinct C-terminus, compared to isoform 2.
      Source sequence(s)
      BB553577, BC052400, BP764506, BY267778, EU034678
      Consensus CDS
      CCDS79732.1
      UniProtKB/TrEMBL
      A2AUP4, Q3U3S6
      Related
      ENSMUSP00000137130.3, ENSMUST00000179584.8
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    5. NM_001177756.1 → NP_001171227.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 5

      See identical proteins and their annotated locations for NP_001171227.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5) lacks one alternate exon in the 3' coding region, compared to variant 2. The resulting isoform (5) has a shorter and distinct C-terminus, compared to isoform 2.
      Source sequence(s)
      BB553577, BC052400, BP764506, BY267778, EU034679
      Consensus CDS
      CCDS50500.1
      UniProtKB/TrEMBL
      A2AUP4, A7UAK8
      Related
      ENSMUSP00000126305.3, ENSMUST00000170196.9
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    6. NM_001177757.1 → NP_001171228.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 7

      See identical proteins and their annotated locations for NP_001171228.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (7) lacks an alternate in-frame exon in the 3' coding region, compared to variant 2. The resulting isoform (7) lacks an internal segment, compared to isoform 2.
      Source sequence(s)
      BB553577, BC052400, BP764506, BY267778
      Consensus CDS
      CCDS50499.1
      UniProtKB/TrEMBL
      A2AUP3, Q7TS91
      Related
      ENSMUSP00000028114.7, ENSMUST00000028114.13
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    7. NM_001177758.1 → NP_001171229.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 8

      Status: VALIDATED

      Description
      Transcript Variant: This variant (8) represents use of an alternate promoter and 5' UTR, uses a distinct translation start codon, and lacks three alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (8) has a distinct N-terminus, lacks an internal segment, and has a shorter and distinct C-terminus, compared to isoform 2.
      Source sequence(s)
      AK143330, AL831794, BC052400, BP764506, EU034678
      Consensus CDS
      CCDS79733.1
      UniProtKB/TrEMBL
      A0A0A6YY64, Q9ESY2
      Related
      ENSMUSP00000142296.2, ENSMUST00000192949.6
      Conserved Domains (2) summary
      pfam00300
      Location:229 → 412
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:4 → 226
      6PF2K; 6-phosphofructo-2-kinase

    8. NM_133232.3 → NP_573495.2  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 6

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6) lacks three alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (6) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 2.
      Source sequence(s)
      BB553577, BC052400, BP764506, BY267778
      Consensus CDS
      CCDS15683.1
      UniProtKB/TrEMBL
      A2AUP1, Q9ESY2
      Related
      ENSMUSP00000110495.3, ENSMUST00000114846.9
      Conserved Domains (2) summary
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      11476241..11558882 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036158939.1 → XP_036014832.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X4

      UniProtKB/TrEMBL
      A2AUP3
      Conserved Domains (2) summary
      pfam00300
      Location:229 → 415
      His_Phos_1; Histidine phosphatase superfamily (branch 1)
      pfam01591
      Location:4 → 226
      6PF2K; 6-phosphofructo-2-kinase

    2. XM_006497367.1 → XP_006497430.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X2

      UniProtKB/TrEMBL
      A2AUP4
      Conserved Domains (2) summary
      pfam01591
      Location:7 → 226
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:229 → 412
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    3. XM_030247921.2 → XP_030103781.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X1

      UniProtKB/TrEMBL
      A2AUP3
      Conserved Domains (2) summary
      pfam01591
      Location:26 → 246
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:249 → 432
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    4. XM_006497369.4 → XP_006497432.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X5

      See identical proteins and their annotated locations for XP_006497432.1

      UniProtKB/TrEMBL
      A2AUP4
      Conserved Domains (2) summary
      pfam01591
      Location:1 → 207
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:210 → 393
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    5. XM_030247930.1 → XP_030103790.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X3

      UniProtKB/TrEMBL
      A2AUP4
      Conserved Domains (2) summary
      pfam01591
      Location:9 → 228
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:231 → 414
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    6. XM_017315954.3 → XP_017171443.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X6

      UniProtKB/TrEMBL
      Q8BVM1, Q9ESY2
      Conserved Domains (2) summary
      pfam01591
      Location:9 → 228
      6PF2K; 6-phosphofructo-2-kinase
      pfam00300
      Location:231 → 414
      His_Phos_1; Histidine phosphatase superfamily (branch 1)

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_172976.1: Suppressed sequence

      Description
      NM_172976.1: This RefSeq was permanently suppressed because it is a nonsense-mediated decay (NMD) candidate.
    Nucleotide Protein Strain
    Heading Accession and Version

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