Gpat4 glycerol-3-phosphate acyltransferase 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gpat4provided by MGI
Official Full Name: glycerol-3-phosphate acyltransferase 4provided by MGI
Primary source: MGI:MGI:2142716
See related: Ensembl:ENSMUSG00000031545 AllianceGenome:MGI:2142716
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Agpat6; Tsarg7
Summary: Enables glycerol-3-phosphate O-acyltransferase activity. Involved in lactation and triglyceride biosynthetic process. Acts upstream of or within several processes, including acylglycerol metabolic process; glandular epithelial cell maturation; and mammary gland development. Located in endoplasmic reticulum. Is expressed in several structures, including adipose tissue; genitourinary system; liver; mammary gland; and spleen. Orthologous to human GPAT4 (glycerol-3-phosphate acyltransferase 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in testis adult (RPKM 56.2), CNS E11.5 (RPKM 13.2) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 A2; 8 11.42 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (23661281..23698469, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (23171265..23208453, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

glycerolipid synthesis is induced during macrophage activation. GPAT4 protein level and GPAT3/GPAT4 enzymatic activity increase during this process, and these two isoforms were required for the accumulation of cell TAG and PL.
Title: Glycerol-3-phosphate acyltransferases 3 and 4 direct glycerolipid synthesis and affect functionality in activated macrophages.
These data identify SEIPIN as an evolutionarily conserved regulator of microsomal GPAT.
Title: SEIPIN Regulates Lipid Droplet Expansion and Adipocyte Development by Modulating the Activity of Glycerol-3-phosphate Acyltransferase.
GPAT4 limits excessive fatty acid oxidation and the detrimental induction of a hypermetabolic state
Title: Glycerol-3-phosphate Acyltransferase Isoform-4 (GPAT4) Limits Oxidation of Exogenous Fatty Acids in Brown Adipocytes.
Data (from studies in knockout mice) suggest GPAT4-derived phosphatidic acid impairs insulin signaling in liver/contributes to insulin resistance via interaction of mTOR (mechanistic target of rapamycin)/Rictor (RPTOR independent companion of mTOR).
Title: Glycerol-3-phosphate acyltransferase-4-deficient mice are protected from diet-induced insulin resistance by the enhanced association of mTOR and rictor.
GPAT1, but not GPAT4, is required to incorporate de novo synthesized fatty acids into TAG and to divert them away from oxidation.
Title: Glycerol-3-phosphate acyltransferase (GPAT)-1, but not GPAT4, incorporates newly synthesized fatty acids into triacylglycerol and diminishes fatty acid oxidation.
Glycerol-3-phosphate acyltransferase 4 might play an important role in spermatogenesis, especially in mid-meiosis.[Glycerol-3-phosphate acyltransferase 4]
Title: Glycerol-3-phosphate acyltransferase 4 gene is involved in mouse spermatogenesis.
"Agpat6(-/-) mice" are actually deficient in a novel NEM-sensitive sn-Glycerol-3-phosphate acyltransferase (GPAT), GPAT4
Title: Identification of a novel sn-glycerol-3-phosphate acyltransferase isoform, GPAT4, as the enzyme deficient in Agpat6-/- mice.
AGPAT6 is a microsomal GPAT, and we propose renaming this enzyme GPAT4.
Title: AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase.
Results identify a novel glycerolipid acyltransferase of the endoplasmic reticulum, AGPAT6, which is crucial for the production of milk fat by the mammary gland.
Title: Agpat6--a novel lipid biosynthetic gene required for triacylglycerol production in mammary epithelium.
Results show that Agpat6 plays a unique role in determining triglyceride content and composition in adipose tissue and liver that cannot be compensated by other members of the Agpat family.
Title: Agpat6 deficiency causes subdermal lipodystrophy and resistance to obesity.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Gene trapped (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU041707 (e-PCR)
- UniSTS:179714

Agpat6 (e-PCR), detects polymorphism
- UniSTS:507965

Agpat6 (e-PCR), detects polymorphism
- UniSTS:546638

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables glycerol-3-phosphate O-acyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycerol-3-phosphate O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables glycerol-3-phosphate O-acyltransferase activity	NAS Non-traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in CDP-diacylglycerol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in acyl-CoA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in acyl-CoA metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within diacylglycerol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fatty acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glandular epithelial cell maturation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lactation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lactation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mammary gland development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylcholine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylcholine biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in triglyceride biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within triglyceride metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	NAS Non-traceable Author Statement more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glycerol-3-phosphate acyltransferase 6

Names: 1-AGP acyltransferase 6; 1-AGPAT 6; 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta); LPAAT-zeta; acyl-CoA:glycerol-3-phosphate acyltransferase 4; lysophosphatidic acid acyltransferase zeta; putative lysophosphatidic acid acyltransferase; testis spermatogenesis apoptosis-related protein 7

NP_061213.2

EC 2.3.1.15

XP_011240406.1

EC 2.3.1.15

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_018743.4 → NP_061213.2 glycerol-3-phosphate acyltransferase 6 precursor

See identical proteins and their annotated locations for NP_061213.2

Status: VALIDATED

Source sequence(s)

AK083589, AK169257, BY032843, BY271836

Consensus CDS

CCDS22189.1

UniProtKB/Swiss-Prot

Q3TF78, Q5QHR4, Q8K2C8

UniProtKB/TrEMBL

Q3TIF6

Related

ENSMUSP00000127325.2, ENSMUST00000167004.3

Conserved Domains (1) summary

cd07991
Location:218 → 427

LPLAT_LPCAT1-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

23661281..23698469 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011242104.4 → XP_011240406.1 glycerol-3-phosphate acyltransferase 6 isoform X1

See identical proteins and their annotated locations for XP_011240406.1

UniProtKB/TrEMBL

Q3TIF6

Conserved Domains (1) summary

cd07991
Location:218 → 433

LPLAT_LPCAT1-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like