Spink1 serine peptidase inhibitor, Kazal type 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spink1provided by MGI
Official Full Name: serine peptidase inhibitor, Kazal type 1provided by MGI
Primary source: MGI:MGI:106202
See related: Ensembl:ENSMUSG00000024503 AllianceGenome:MGI:106202
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: p12; Spink3
Summary: Enables serine-type endopeptidase inhibitor activity. Acts upstream of or within several processes, including negative regulation of protein metabolic process; regulation of calcium ion transport; and sperm capacitation. Located in extracellular space. Is expressed in several structures, including embryo ectoderm; genitourinary system; gut; liver; and yolk sac. Human ortholog(s) of this gene implicated in pancreatitis. Orthologous to human SPINK1 (serine peptidase inhibitor Kazal type 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in kidney adult (RPKM 309.1), placenta adult (RPKM 259.9) and 7 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 B3; 18 23.47 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (43861134..43870302, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (43728069..43737237, complement)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Heterozygous Spink1 c.194+2T>C mutation promotes chronic pancreatitis after acute attack in mice.
Title: Heterozygous Spink1 c.194+2T>C mutation promotes chronic pancreatitis after acute attack in mice.
A mouse testis serine protease, TESP1, as the potential SPINK3 receptor protein on mouse sperm acrosome.
Title: A mouse testis serine protease, TESP1, as the potential SPINK3 receptor protein on mouse sperm acrosome.
reduces multi-organ injury caused by gut ischemia/reperfusion
Title: Pancreatic secretory trypsin inhibitor reduces multi-organ injury caused by gut ischemia/reperfusion in mice.
It is a trypsin-specific inhibitor in the pancreas.
Title: Generation and analysis of serine protease inhibitor kazal type 3-cre driver mice.
SPINK3 modulates sperm physiology through a downstream reduction of endogenous NO concentration and independently of SPINK3 trypsin inhibitory activity.
Title: SPINK3 modulates mouse sperm physiology through the reduction of nitric oxide level independently of its trypsin inhibitory activity.
IFN regulatory factor 2 (Irf2) has a regulatory role in trypsinogen5 gene transcription, which is resistant to a major endogenous trypsin inhibitor, Spink3
Title: Characterization of dsRNA-induced pancreatitis model reveals the regulatory role of IFN regulatory factor 2 (Irf2) in trypsinogen5 gene transcription.
uterine-gland-derived SPINK3, as a new paracrine modulator, might play an important role in embryo implantation through its influence on stromal decidualization in mice.
Title: Role of secretory protease inhibitor SPINK3 in mouse uterus during early pregnancy.
Spink3 may act as a growth factor, leading to cell proliferation and regeneration of tissue structure.
Title: Chronic pancreatitis in mice by treatment with choline-deficient ethionine-supplemented diet.
Results suggest that regulation of Prss1 and Spink3 expression is involved in the susceptibility to experimentally induced pancreatitis.
Title: Relationship of strain-dependent susceptibility to experimentally induced acute pancreatitis with regulation of Prss1 and Spink3 expression.
Spink3 trypsin inhibitor is required for normal pancreatic development. Data indicate an important relationship between endogenous pancreatic trypsin and Spink3 and the development of pancreatitis.
Title: Transgenic expression of pancreatic secretory trypsin inhibitor-1 rescues SPINK3-deficient mice and restores a normal pancreatic phenotype.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Spink3 (e-PCR), detects polymorphism
- UniSTS:516152

Spink3 (e-PCR), detects polymorphism
- UniSTS:463472

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables peptidase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables serine-type endopeptidase inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type endopeptidase inhibitor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of calcium ion import	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of nitric oxide mediated signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of peptidase activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of serine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of serine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of nitric oxide mediated signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of peptidyl-tyrosine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of acrosome reaction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of store-operated calcium entry	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within sperm capacitation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine protease inhibitor Kazal-type 1

Names: prostatic secretory glycoprotein; serine peptidase inhibitor, Kazal type 3; serine protease inhibitor Kazal-type 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009258.5 → NP_033284.1 serine protease inhibitor Kazal-type 1 precursor

See identical proteins and their annotated locations for NP_033284.1

Status: VALIDATED

Source sequence(s)

AV048854, CA481615

Consensus CDS

CCDS37803.1

UniProtKB/Swiss-Prot

P09036, Q5M9M3

Related

ENSMUSP00000025381.3, ENSMUST00000025381.4

Conserved Domains (1) summary

cd01327
Location:39 → 80

KAZAL_PSTI; Kazal-type pancreatic secretory trypsin inhibitors (PSTI) and related proteins, including the second domain of the ovomucoid turkey inhibitor and the C-terminal domain of the esophagus cancer-related gene-2 protein (ECRG-2), are members of the ...