Alox5ap arachidonate 5-lipoxygenase activating protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Alox5approvided by MGI
Official Full Name: arachidonate 5-lipoxygenase activating proteinprovided by MGI
Primary source: MGI:MGI:107505
See related: Ensembl:ENSMUSG00000060063 AllianceGenome:MGI:107505
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Flap
Summary: Predicted to enable several functions, including arachidonate binding activity; glutathione peroxidase activity; and glutathione transferase activity. Predicted to contribute to arachidonate 5-lipoxygenase activity. Acts upstream of or within leukotriene biosynthetic process and leukotriene production involved in inflammatory response. Located in nucleus. Is expressed in cerebral cortex; hemolymphoid system; limb long bone; liver; and vertebra. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); asthma; brain infarction (multiple); breast cancer; and cerebral palsy. Orthologous to human ALOX5AP (arachidonate 5-lipoxygenase activating protein). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in liver E18 (RPKM 51.8), lung adult (RPKM 33.9) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G3; 5 89.18 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (149201814..149224963)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (149265004..149288153)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Within the endothelial population, cells that transition from haemogenic endothelial to erythro-myeloid progenitors specifically express Alox5 and its co-factor Alox5ap, which control leukotriene production. Functional assays using mouse embryonic stem cells demonstrate that leukotrienes promote haematopoietic progenitor cell generation.
Title: Defining murine organogenesis at single-cell resolution reveals a role for the leukotriene pathway in regulating blood progenitor formation.
ALOX5AP Overexpression in Adipose Tissue Leads to LXA4 Production and Protection Against Diet-Induced Obesity and Insulin Resistance
Title: ALOX5AP Overexpression in Adipose Tissue Leads to LXA4 Production and Protection Against Diet-Induced Obesity and Insulin Resistance.
Data indicate that a single amino acid mutation was sufficient to reverse the speciation observed in wild type 5-Lipoxygenase activating protein (FLAP).
Title: A Single Amino Acid Difference between Mouse and Human 5-Lipoxygenase Activating Protein (FLAP) Explains the Speciation and Differential Pharmacology of Novel FLAP Inhibitors.
BRP-7 potently suppresses leukotriene biosynthesis by interacting with FLAP in mouse peritonitis model.
Title: The novel benzimidazole derivative BRP-7 inhibits leukotriene biosynthesis in vitro and in vivo by targeting 5-lipoxygenase-activating protein (FLAP).
FLAP regulates phosphorylation of tau protein via modulating GSK-3beta activity.
Title: FLAP pharmacological blockade modulates metabolism of endogenous tau in vivo .
This study demonistrated that a a novel role for FLAP in the pathogenesis of anxiety-like behavior in mice.
Title: The involvement of 5-lipoxygenase activating protein in anxiety-like behavior.
Deletion of myeloid cell FLAP markedly attenuates the proliferative response to vascular injury, while preserving endothelial integrity.
Title: Myeloid cell 5-lipoxygenase activating protein modulates the response to vascular injury.
A FLAP inhibitor given during critical window periods may prevent oxygen induced aberration of pulmonary alveolarization in a dose- and time-dependent manner.
Title: 5-Lipoxygenase-activating protein (FLAP) inhibitor MK-0591 prevents aberrant alveolarization in newborn mice exposed to 85% oxygen in a dose- and time-dependent manner.
the 5-LO pathway emerges early during embryonic stem cell differentiation into cells of the myeloid lineage
Title: The 5-lipoxygenase pathway regulates vasculogenesis in differentiating mouse embryonic stem cells.
Adipose tissue from obese mice, compared with that from lean mice, exhibited increased 5-LO activating protein
Title: 5-lipoxygenase activating protein signals adipose tissue inflammation and lipid dysfunction in experimental obesity.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Alox5ap (e-PCR), detects polymorphism
- UniSTS:526778

Alox5ap (e-PCR), detects polymorphism
- UniSTS:526779

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables arachidonate 5-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
contributes_to arachidonate 5-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables arachidonate binding	ISO Inferred from Sequence Orthology more info
enables arachidonate binding	ISS Inferred from Sequence or Structural Similarity more info
enables enzyme activator activity	IEA Inferred from Electronic Annotation more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables glutathione peroxidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutathione transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables leukotriene-C4 synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to calcium ion	IEA Inferred from Electronic Annotation more info
involved_in cellular response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in leukotriene biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within leukotriene biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within leukotriene biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in leukotriene biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within leukotriene production involved in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipoxygenase pathway	IEA Inferred from Electronic Annotation more info
involved_in lipoxygenase pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of acute inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of acute inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in protein homotrimerization	IEA Inferred from Electronic Annotation more info
involved_in protein homotrimerization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear envelope	ISO Inferred from Sequence Orthology more info
located_in nuclear envelope	ISS Inferred from Sequence or Structural Similarity more info
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
located_in nuclear membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: arachidonate 5-lipoxygenase-activating protein

Names: MK-886-binding protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001308462.1 → NP_001295391.1 arachidonate 5-lipoxygenase-activating protein isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 3' UTR, and lacks an alternate exon in the 5' coding region resulting in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.

Source sequence(s)

AC122546, AC175377
NM_009663.2 → NP_033793.1 arachidonate 5-lipoxygenase-activating protein isoform 1

See identical proteins and their annotated locations for NP_033793.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK004002, BC026209, BE944409, BG085800

Consensus CDS

CCDS39407.1

UniProtKB/Swiss-Prot

P30355, Q9D138

Related

ENSMUSP00000071130.4, ENSMUST00000071130.5

Conserved Domains (1) summary

pfam01124
Location:9 → 128

MAPEG; MAPEG family