Epn2 epsin 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Epn2provided by RGD
Official Full Name: epsin 2provided by RGD
Primary source: RGD:619773
See related: EnsemblRapid:ENSRNOG00000060550 AllianceGenome:RGD:619773
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: The protein encoded by this gene is a member of the epsin protein family. Epsin proteins are endocytic adaptors that function in the formation of clatherin-coated vesicles. Epsins contain a highly conserved N-terminal homology domain that binds phosphatidylinositol 4,5-bisphosphate in the plasma membrane, two or three ubiquitin interacting motifs, two clathrin-binding motifs, a cluster of aspartate-proline-tryptophan/phenylalanine repeats, and two or three asparagine-proline-phenylalanine tripeptide repeats at the C-terminus. In mouse, simultaneous knockout of this gene and its paralog results in embryonic arrest due to disruption of Notch signaling, suggesting a role as a specialized endocytic adaptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Expression: Biased expression in Brain (RPKM 238.6), Lung (RPKM 228.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q22

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (46697238..46759128, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (46197785..46259673, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (47795496..47857373, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BE113852 (e-PCR)
- UniSTS:246820

RH141144 (e-PCR)
- UniSTS:223140

RH139797 (e-PCR)
- UniSTS:221881

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables clathrin binding	IBA Inferred from Biological aspect of Ancestor more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Notch signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within embryonic organ development	ISO Inferred from Sequence Orthology more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within in utero embryonic development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of sprouting angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular endothelial growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of vascular endothelial growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of Notch signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of Notch signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of endocytosis	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of clathrin coat of endocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
part_of clathrin vesicle coat	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in postsynaptic endocytic zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic endocytic zone	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: epsin-2

Names: EH domain binding protein epsin 2; EPS-15-interacting protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001033914.2 → NP_001029086.1 epsin-2 isoform b

See identical proteins and their annotated locations for NP_001029086.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 3. Variants 2 and 3 encode isoform b.

Source sequence(s)

BC094524, CB583573, JAXUCZ010000010

UniProtKB/TrEMBL

A6HF84, Q505I9

Conserved Domains (1) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
NM_001305628.1 → NP_001292557.1 epsin-2 isoform b

See identical proteins and their annotated locations for NP_001292557.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (b). Variants 2 and 3 encode the same isoform (b).

Source sequence(s)

BC094524, CB583573, JAXUCZ010000010

UniProtKB/TrEMBL

A6HF84, Q505I9

Related

ENSRNOP00000074461.1, ENSRNOT00000085703.3

Conserved Domains (1) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
NM_021852.3 → NP_068624.2 epsin-2 isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (1) differs in its 5' UTR and lacks an alternate in-frame exon in the 5' coding region compared to variant 3. It encodes isoform a, which is shorter than isoform b.

Source sequence(s)

BC094524, JAXUCZ010000010

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:335 → 399

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

46697238..46759128 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039086747.2 → XP_038942675.1 epsin-2 isoform X3

UniProtKB/TrEMBL

A6HF84, F1LQ45

Related

ENSRNOP00000106806.1, ENSRNOT00000155858.1

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:347 → 411

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_039086748.2 → XP_038942676.1 epsin-2 isoform X4

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:335 → 399

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_006246521.5 → XP_006246583.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_039086749.2 → XP_038942677.1 epsin-2 isoform X4

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:335 → 399

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269770.1 → XP_063125840.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_017597507.3 → XP_017452996.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Related

ENSRNOP00000073697.3, ENSRNOT00000091963.3

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269779.1 → XP_063125849.1 epsin-2 isoform X4
XM_017597506.3 → XP_017452995.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269768.1 → XP_063125838.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_063269767.1 → XP_063125837.1 epsin-2 isoform X2

UniProtKB/TrEMBL

Q505I9
XM_017597511.2 → XP_017453000.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269775.1 → XP_063125845.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_039086746.2 → XP_038942674.1 epsin-2 isoform X2

UniProtKB/TrEMBL

A6HF84, Q505I9

Conserved Domains (1) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
XM_063269781.1 → XP_063125851.1 epsin-2 isoform X4
XM_039086741.2 → XP_038942669.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_039086743.2 → XP_038942671.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_017597508.3 → XP_017452997.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269776.1 → XP_063125846.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_063269772.1 → XP_063125842.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_063269782.1 → XP_063125852.1 epsin-2 isoform X4
XM_063269780.1 → XP_063125850.1 epsin-2 isoform X4
XM_039086751.2 → XP_038942679.1 epsin-2 isoform X5
XM_039086745.2 → XP_038942673.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_039086742.2 → XP_038942670.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269765.1 → XP_063125835.1 epsin-2 isoform X1
XM_063269769.1 → XP_063125839.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_039086740.2 → XP_038942668.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269766.1 → XP_063125836.1 epsin-2 isoform X1
XM_063269764.1 → XP_063125834.1 epsin-2 isoform X1
XM_039086744.2 → XP_038942672.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_017597510.3 → XP_017452999.1 epsin-2 isoform X1

UniProtKB/TrEMBL

A6HF84

Conserved Domains (2) summary

cd16990
Location:19 → 141

ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

cl00427
Location:404 → 468

TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...
XM_063269778.1 → XP_063125848.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_063269774.1 → XP_063125844.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_063269777.1 → XP_063125847.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_063269773.1 → XP_063125843.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45
XM_063269783.1 → XP_063125853.1 epsin-2 isoform X4
XM_063269771.1 → XP_063125841.1 epsin-2 isoform X3

UniProtKB/TrEMBL

F1LQ45