Cpb2 carboxypeptidase B2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cpb2provided by MGI
Official Full Name: carboxypeptidase B2provided by MGI
Primary source: MGI:MGI:1891837
See related: Ensembl:ENSMUSG00000021999 AllianceGenome:MGI:1891837
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CPR; Cpu; TAFI; 1110032P04Rik; 4930405E17Rik
Summary: This gene encodes carboxypeptidase B, a zinc-dependent metalloprotease that cleaves peptide bonds at the C-terminus of protein substrates. The encoded preproprotein undergoes proteolytic activation to generate a mature, functional enzyme, and secreted into plasma. [provided by RefSeq, Jan 2016]
Expression: Biased expression in liver E18 (RPKM 213.1), liver adult (RPKM 49.9) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 D3; 14 39.73 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (75479727..75520995)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (75242287..75283555)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Brain Expression of CPB2 and Effects of Cpb2 Deficiency in Mouse Models of Behavior.
Title: Brain Expression of CPB2 and Effects of Cpb2 Deficiency in Mouse Models of Behavior.
Both plasma basic carboxypeptidases, carboxypeptidase B2 and carboxypeptidase N, regulate vascular leakage activity in mice.
Title: Both plasma basic carboxypeptidases, carboxypeptidase B2 and carboxypeptidase N, regulate vascular leakage activity in mice.
Activated thrombin-activatable fibrinolysis inhibitor (TAFIa) attenuates fibrin-dependent plasmin generation on thrombin-activated platelets.
Title: Activated thrombin-activatable fibrinolysis inhibitor (TAFIa) attenuates fibrin-dependent plasmin generation on thrombin-activated platelets.
TAFI deficiency causes maladaptive vascular remodeling after hemophilic joint bleeding.
Title: TAFI deficiency causes maladaptive vascular remodeling after hemophilic joint bleeding.
CPB2 functions as an on-demand supplementary anaphylatoxin inhibitor in inactivating excessive C5a formed locally.
Title: Carboxypeptidase B2 and N play different roles in regulation of activated complements C3a and C5a in mice.
These data identify TAFI as an important modifier of hemophilic joint bleeding in acquired hemophilia A by inhibiting uPA-mediated fibrinolysis.
Title: Defective TAFI activation in hemophilia A mice is a major contributor to joint bleeding.
Results indicate that TAFIa could be a novel biomarker and realistic therapeutic target of chronic thromboembolic pulmonary hypertension.
Title: Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Target.
Cpb2-deficient mice had decreased pneumococcal meningitis mortality and attenuated cytokine levels and bacterial outgrowth in the systemic compartment but not in the brain compartment, as compared with wild-type mice.
Title: Thrombin-activatable fibrinolysis inhibitor influences disease severity in humans and mice with pneumococcal meningitis.
Carboxypeptidase B2 deficiency reveals that complement C3a limits infection and C5a exacerbates infection in a murine polymicrobial sepsis model.
Title: Carboxypeptidase B2 deficiency reveals opposite effects of complement C3a and C5a in a murine polymicrobial sepsis model.
Combination of TAFI-I and a low dose of rtPA was not as effective as the standard dose of rtPA in treating thromboembolic stroke, while TAFI inhibition alone was not effective at all.
Title: Effects of a TAFI-inhibitor combined with a suboptimal dose of rtPA in a murine thromboembolic model of stroke.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC150816P1 (e-PCR)
- UniSTS:271105

Cpb2 (e-PCR), detects polymorphism
- UniSTS:525890

Cpb2 (e-PCR), detects polymorphism
- UniSTS:506811

AI255929 (e-PCR)
- UniSTS:180700

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carboxypeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables carboxypeptidase activity	ISO Inferred from Sequence Orthology more info
enables metallocarboxypeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallocarboxypeptidase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in blood coagulation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to glucose stimulus	IEA Inferred from Electronic Annotation more info
involved_in fibrinolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in fibrinolysis	IEA Inferred from Electronic Annotation more info
involved_in fibrinolysis	ISO Inferred from Sequence Orthology more info
involved_in liver regeneration	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of fibrinolysis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of fibrinolysis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of hepatocyte proliferation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of hepatocyte proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of plasminogen activation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of plasminogen activation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of extracellular matrix constituent secretion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of extracellular matrix constituent secretion	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: carboxypeptidase B2

Names: carboxypeptidase B2 (plasma); carboxypeptidase R; carboxypeptidase U; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor

NP_062749.2

EC 3.4.17.20

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019775.3 → NP_062749.2 carboxypeptidase B2 preproprotein

See identical proteins and their annotated locations for NP_062749.2

Status: REVIEWED

Source sequence(s)

AA048136, AK004045, CO807338

Consensus CDS

CCDS27277.1

UniProtKB/Swiss-Prot

Q5EBI3, Q9JHH6, Q9QZF0

Related

ENSMUSP00000022576.9, ENSMUST00000022576.10

Conserved Domains (2) summary

cd06246
Location:118 → 419

M14_CPB2; Peptidase M14 carboxypeptidase subfamily A/B-like; Carboxypeptidase B2 subgroup

pfam02244
Location:32 → 103

Propep_M14; Carboxypeptidase activation peptide