Qki quaking, KH domain containing RNA binding [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Qkiprovided by MGI
Official Full Name: quaking, KH domain containing RNA bindingprovided by MGI
Primary source: MGI:MGI:97837
See related: Ensembl:ENSMUSG00000062078 AllianceGenome:MGI:97837
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Qk; l17Wis1; 1500005P18; l(17)-1Wis; 1110003F05Rik
Summary: Enables mRNA 3'-UTR binding activity and transcription coactivator activity. Involved in several processes, including negative regulation of metabolic process; regulation of glial cell differentiation; and regulation of mRNA metabolic process. Acts upstream of or within several processes, including long-chain fatty acid biosynthetic process; spermatid development; and vasculature development. Located in cytoplasm and nucleus. Is active in synapse. Is expressed in several structures, including central nervous system; craniocervical region bone; reproductive system; sensory organ; and yolk sac. Orthologous to human QKI (QKI, KH domain containing RNA binding). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in frontal lobe adult (RPKM 30.8), cerebellum adult (RPKM 26.6) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A1; 17 7.75 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (10425480..10538706, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (10206471..10319361, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Qki5 safeguards spinal motor neuron function by defining the motor neuron-specific transcriptome via pre-mRNA processing.
Title: Qki5 safeguards spinal motor neuron function by defining the motor neuron-specific transcriptome via pre-mRNA processing.
RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway.
Title: RNA-binding Protein QKI Inhibits Osteogenic Differentiation Via Suppressing Wnt Pathway.
The RNA-binding protein QKI governs a muscle-specific alternative splicing program that shapes the contractile function of cardiomyocytes.
Title: The RNA-binding protein QKI governs a muscle-specific alternative splicing program that shapes the contractile function of cardiomyocytes.
QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.
Title: QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.
LncRNA CCAT1 enhances chemoresistance in hepatocellular carcinoma by targeting QKI-5.
Title: LncRNA CCAT1 enhances chemoresistance in hepatocellular carcinoma by targeting QKI-5.
Muscle stem cell polarity requires QKI-mediated alternative splicing of Integrin Alpha-7 (Itga7).
Title: Muscle stem cell polarity requires QKI-mediated alternative splicing of Integrin Alpha-7 (Itga7).
Qki regulates myelinogenesis through Srebp2-dependent cholesterol biosynthesis.
Title: Qki regulates myelinogenesis through Srebp2-dependent cholesterol biosynthesis.
QUAKING Regulates Microexon Alternative Splicing of the Rho GTPase Pathway and Controls Microglia Homeostasis.
Title: QUAKING Regulates Microexon Alternative Splicing of the Rho GTPase Pathway and Controls Microglia Homeostasis.
Qki is an essential regulator of microglial phagocytosis in demyelination.
Title: Qki is an essential regulator of microglial phagocytosis in demyelination.
Identification of Qk as a Glial Precursor Cell Marker that Governs the Fate Specification of Neural Stem Cells to a Glial Cell Lineage.
Title: Identification of Qk as a Glial Precursor Cell Marker that Governs the Fate Specification of Neural Stem Cells to a Glial Cell Lineage.

Submit: New GeneRIF Correction See all GeneRIFs (50)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (5) 1 citation
Endonuclease-mediated (7)
Gene trapped (2) 1 citation
Spontaneous (3) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:237569

Qk (e-PCR), detects polymorphism
- UniSTS:462630

G67016 (e-PCR)
- UniSTS:178222

Qk (e-PCR), detects polymorphism
- UniSTS:464688

D6S1171E (e-PCR)
- UniSTS:151092

BE111947 (e-PCR)
- UniSTS:245820

D17Mit58 (e-PCR), detects polymorphism
- UniSTS:128092

Dep1 (e-PCR), detects polymorphism
- UniSTS:256609

Qk (e-PCR), detects polymorphism
- UniSTS:527511

D17Jcs113 (e-PCR), detects polymorphism
- UniSTS:259229

D17Jcs114 (e-PCR), detects polymorphism
- UniSTS:259230

QKI (e-PCR)
- UniSTS:266175

AW556556 (e-PCR)
- UniSTS:186083

Qk (e-PCR), detects polymorphism
- UniSTS:479216

PMC169897P1 (e-PCR)
- UniSTS:271611

PMC169897P2 (e-PCR)
- UniSTS:271612

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	TAS Traceable Author Statement more info	PubMed
enables SH3 domain binding	IEA Inferred from Electronic Annotation more info
enables internal N(7)-methylguanine-containing RNA reader activity	IEA Inferred from Electronic Annotation more info
enables internal N(7)-methylguanine-containing RNA reader activity	ISO Inferred from Sequence Orthology more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA 3'-UTR binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables mRNA 3'-UTR binding	ISO Inferred from Sequence Orthology more info
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables mRNA binding	ISO Inferred from Sequence Orthology more info
enables miRNA binding	ISO Inferred from Sequence Orthology more info
enables miRNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables transcription coactivator activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within axon ensheathment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	IEA Inferred from Electronic Annotation more info
involved_in intracellular mRNA localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within long-chain fatty acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	ISO Inferred from Sequence Orthology more info
involved_in mRNA stabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA stabilization	ISO Inferred from Sequence Orthology more info
involved_in mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in mRNA transport	ISO Inferred from Sequence Orthology more info
involved_in microglia differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within myelination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in myofibroblast contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of 3'-UTR-mediated mRNA stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of angiogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cardiac muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cold-induced thermogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of macrophage differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of macrophage differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of miRNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of miRNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of translation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of type I interferon production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of type I interferon production	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of 3'-UTR-mediated mRNA stabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cholesterol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of myelination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of oligodendrocyte differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of oligodendrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of astrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of epithelial to mesenchymal transition	ISO Inferred from Sequence Orthology more info
involved_in regulation of epithelial to mesenchymal transition	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of mRNA splicing, via spliceosome	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mRNA splicing, via spliceosome	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mRNA splicing, via spliceosome	ISO Inferred from Sequence Orthology more info
involved_in regulation of macrophage differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within spermatid development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in spliceosome-depend formation of circular RNA	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within vascular associated smooth muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vascular associated smooth muscle cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in vascular associated smooth muscle cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within vasculogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	TAS Traceable Author Statement more info	PubMed
located_in cytoplasmic stress granule	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasmic stress granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	TAS Traceable Author Statement more info	PubMed
is_active_in synapse	EXP Inferred from Experiment more info	PubMed
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: KH domain-containing RNA-binding protein QKI

Names: QKI-5 protein; QKI-6 protein; QKI-7 protein; QKI-7b protein; lethal, Chr 17, U Wisconsin 1; protein quaking; quaking

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001159516.2 → NP_001152988.1 KH domain-containing RNA-binding protein QKI isoform 2

See identical proteins and their annotated locations for NP_001152988.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AC113114, AC154238

Consensus CDS

CCDS88989.1

UniProtKB/Swiss-Prot

Q9QYS9

Related

ENSMUSP00000156665.2, ENSMUST00000233828.2

Conserved Domains (3) summary

PRK14951
Location:210 → 281

PRK14951; DNA polymerase III subunits gamma and tau; Provisional

pfam16544
Location:10 → 66

STAR_dimer; Homodimerization region of STAR domain protein

cd22465
Location:81 → 183

KH-I_Hqk; type I K homology (KH) RNA-binding domain found in protein quaking (Hqk) and similar proteins
NM_001159517.2 → NP_001152989.1 KH domain-containing RNA-binding protein QKI isoform 1

See identical proteins and their annotated locations for NP_001152989.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC113114, AC154238

Consensus CDS

CCDS49947.1

UniProtKB/Swiss-Prot

O88972, Q61110, Q78ZE4, Q78ZE5, Q8K4X9, Q8K4Y0, Q9CW34, Q9QUH4, Q9QYS9, Q9R2A8, Q9Z246

Related

ENSMUSP00000156511.2, ENSMUST00000233645.2

Conserved Domains (4) summary

PHA03247
Location:211 → 314

PHA03247; large tegument protein UL36; Provisional

pfam16544
Location:10 → 66

STAR_dimer; Homodimerization region of STAR domain protein

pfam16551
Location:312 → 341

Quaking_NLS; Putative nuclear localization signal of quaking

cd22465
Location:81 → 183

KH-I_Hqk; type I K homology (KH) RNA-binding domain found in protein quaking (Hqk) and similar proteins
NM_021881.3 → NP_068681.1 KH domain-containing RNA-binding protein QKI isoform 3

See identical proteins and their annotated locations for NP_068681.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.

Source sequence(s)

AC113114, AC154238

Consensus CDS

CCDS28387.1

UniProtKB/Swiss-Prot

Q9QYS9

Related

ENSMUSP00000046740.8, ENSMUST00000042296.9

Conserved Domains (3) summary

PRK14951
Location:210 → 281

PRK14951; DNA polymerase III subunits gamma and tau; Provisional

pfam16544
Location:10 → 66

STAR_dimer; Homodimerization region of STAR domain protein

cd22465
Location:81 → 183

KH-I_Hqk; type I K homology (KH) RNA-binding domain found in protein quaking (Hqk) and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

10425480..10538706 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006523300.4 → XP_006523363.1 KH domain-containing RNA-binding protein QKI isoform X1

See identical proteins and their annotated locations for XP_006523363.1

Conserved Domains (4) summary

PRK14950
Location:195 → 305

PRK14950; DNA polymerase III subunits gamma and tau; Provisional

pfam16544
Location:10 → 66

STAR_dimer; Homodimerization region of STAR domain protein

pfam16551
Location:304 → 333

Quaking_NLS; Putative nuclear localization signal of quaking

cd22465
Location:81 → 183

KH-I_Hqk; type I K homology (KH) RNA-binding domain found in protein quaking (Hqk) and similar proteins
XM_030249598.2 → XP_030105458.1 KH domain-containing RNA-binding protein QKI isoform X2

Conserved Domains (3) summary

PRK14971
Location:182 → 311

PRK14971; DNA polymerase III subunit gamma/tau

pfam16544
Location:10 → 66

STAR_dimer; Homodimerization region of STAR domain protein

cd22465
Location:81 → 183

KH-I_Hqk; type I K homology (KH) RNA-binding domain found in protein quaking (Hqk) and similar proteins