U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    ANAPC2 anaphase promoting complex subunit 2 [ Homo sapiens (human) ]

    Gene ID: 29882, updated on 10-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    ANAPC2provided by HGNC
    Official Full Name
    anaphase promoting complex subunit 2provided by HGNC
    Primary source
    HGNC:HGNC:19989
    See related
    Ensembl:ENSG00000176248 MIM:606946; AllianceGenome:HGNC:19989
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    APC2
    Summary
    A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in testis (RPKM 16.5), spleen (RPKM 12.2) and 25 other tissues See more
    Orthologs
    mouse all
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See ANAPC2 in Genome Data Viewer
    Location:
    9q34.3
    Exon count:
    14
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 9 NC_000009.12 (137174784..137188560, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 9 NC_060933.1 (149412464..149426223, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (140069236..140083012, complement)

    Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20586 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20587 Neighboring gene leucine rich repeat containing 26 Neighboring gene microRNA 3621 Neighboring gene uncharacterized LOC124902315 Neighboring gene transmembrane protein 210 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:140069251-140070084 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29347 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20588 Neighboring gene SS nuclear autoantigen 1 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:140088338-140089216 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:140089217-140090094 Neighboring gene taperin Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20589 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20590

    Genomic regions, transcripts, and products

    Go to the top of the page Help

    Go to nucleotide: Graphics FASTA GenBank

    Expression

    Go to the top of the page Help
    • Project title: Tissue-specific circular RNA induction during human fetal development
    • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
    • BioProject: PRJNA270632
    • Publication: PMID 26076956
    • Analysis date: Mon Apr 2 22:54:59 2018

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Orf virus cell cycle regulator, PACR, competes with subunit 11 of the anaphase promoting complex for incorporation into the complex. Mo M, et al. J Gen Virol, 2010 Dec. PMID 20826619
    2. The expression pattern of APC2 and APC7 in various cancer cell lines and AML patients. Rahimi H, et al. Adv Med Sci, 2015 Sep. PMID 26046517
    3. SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs. van der Lelij P, et al. EMBO J, 2014 Nov 18. PMID 25257309, Free PMC Article
    4. DNA-PKcs Negatively Regulates Cyclin B1 Protein Stability through Facilitating Its Ubiquitination Mediated by Cdh1-APC/C Pathway. Shang ZF, et al. Int J Biol Sci, 2015. PMID 26221070, Free PMC Article
    5. Anaphase-promoting complex/cyclosome protein Cdc27 is a target for curcumin-induced cell cycle arrest and apoptosis. Lee SJ, et al. BMC Cancer, 2012 Jan 26. PMID 22280307, Free PMC Article

    See all (131) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The authors showed, in vitro, a direct interaction between Orf virus anaphase promoting complex regulator and APC2 and its interference with interactions between APC11 and APC2.
      Title: Orf virus cell cycle regulator, PACR, competes with subunit 11 of the anaphase promoting complex for incorporation into the complex.
    2. A transcriptome-wide analysis revealed that SNW1 or PRPF8 depletion affects the splicing of specific introns in a subset of pre-mRNAs, including pre-mRNAs encoding the cohesion protein sororin and the APC/C subunit APC2.
      Title: SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs.
    3. The results showed that APC2 and APC7 subunits were both over expressed in cancer cell lines
      Title: The expression pattern of APC2 and APC7 in various cancer cell lines and AML patients.
    4. The regulation of Mdm2 by the E3 ubiquitin ligase APC/C is shown. It has important therapeutic implications for tumors with Mdm2 overexpression.
      Title: The anaphase-promoting complex/cyclosome is an E3 ubiquitin ligase for Mdm2.
    5. anaphase-promoting complex (APC)-2-cell cycle and apoptosis regulatory protein (CARP)-1 interaction antagonists are novel regulators of cell growth and apoptosis
      Title: Antagonists of anaphase-promoting complex (APC)-2-cell cycle and apoptosis regulatory protein (CARP)-1 interaction are novel regulators of cell growth and apoptosis.
    6. It proposes a role for APC/C(Cdh1) in modulating the status of PCNA monoubiquitination and UV DNA repair before S phase entry.
      Title: APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage.
    7. APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex.
      Title: APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex.
    8. Results describe the locations of Cdh1 and Apc2 in the anaphase-promoting complex/cyclosome of human and Xenopus laevis.
      Title: Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C.
    Submit: New GeneRIF Correction

    Phenotypes

    Go to the top of the page Help

    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

    Go to the top of the page Help

    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    HIV-1 interactions

    Go to the top of the page Help

    Replication interactions

    Interaction Pubs
    Knockdown of anaphase promoting complex subunit 2 (ANAPC2) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed
    Knockdown of anaphase promoting complex subunit 2 (ANAPC2) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Pol gag-pol HIV-1 Pol is identified to have a physical interaction with anaphase promoting complex subunit 2 (ANAPC2) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
    retropepsin gag-pol HIV-1 PR is identified to have a physical interaction with anaphase promoting complex subunit 2 (ANAPC2) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin protein ligase binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in anaphase-promoting complex-dependent catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in anaphase-promoting complex-dependent catabolic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cell differentiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in metaphase/anaphase transition of mitotic cell cycle IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in negative regulation of gene expression IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nervous system development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of axon extension IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of dendrite morphogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of synapse maturation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in positive regulation of synaptic plasticity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in protein K11-linked ubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein K11-linked ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein K48-linked ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein branched polyubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of meiotic cell cycle NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of mitotic cell cycle NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of anaphase-promoting complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of anaphase-promoting complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of anaphase-promoting complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of anaphase-promoting complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    anaphase-promoting complex subunit 2
    Names
    cyclosome subunit 2

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_013366.4 → NP_037498.1  anaphase-promoting complex subunit 2

      See identical proteins and their annotated locations for NP_037498.1

      Status: REVIEWED

      Source sequence(s)
      BC032503, BM925063
      Consensus CDS
      CCDS7033.1
      UniProtKB/Swiss-Prot
      Q5VSG1, Q96DG5, Q96GG4, Q9P2E1, Q9UJX6
      Related
      ENSP00000314004.2, ENST00000323927.3
      Conserved Domains (2) summary
      smart01013
      Location:757 → 817
      APC2; Anaphase promoting complex (APC) subunit 2
      pfam00888
      Location:512 → 694
      Cullin; Cullin family

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000009.12 Reference GRCh38.p14 Primary Assembly

      Range
      137174784..137188560 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047423275.1 → XP_047279231.1  anaphase-promoting complex subunit 2 isoform X2

      UniProtKB/TrEMBL
      B4DGH7

    2. XM_047423274.1 → XP_047279230.1  anaphase-promoting complex subunit 2 isoform X1

      UniProtKB/TrEMBL
      B4DGH7

    3. XM_047423276.1 → XP_047279232.1  anaphase-promoting complex subunit 2 isoform X3

      UniProtKB/TrEMBL
      B4DGH7

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060933.1 Alternate T2T-CHM13v2.0

      Range
      149412464..149426223 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054362821.1 → XP_054218796.1  anaphase-promoting complex subunit 2 isoform X2

      UniProtKB/TrEMBL
      B4DGH7

    2. XM_054362820.1 → XP_054218795.1  anaphase-promoting complex subunit 2 isoform X1

      UniProtKB/TrEMBL
      B4DGH7

    3. XM_054362822.1 → XP_054218797.1  anaphase-promoting complex subunit 2 isoform X3

      UniProtKB/TrEMBL
      B4DGH7
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9UJX6.1 GenPept UniProtKB/Swiss-Prot:Q9UJX6

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous