Lnpep leucyl and cystinyl aminopeptidase [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Lnpepprovided by RGD
Full Name: leucyl and cystinyl aminopeptidaseprovided by RGD
Primary source: RGD:621752
See related: EnsemblRapid:ENSRNOG00000055229 AllianceGenome:RGD:621752
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: IRAP; GP160; OTase; P-LAP; Vp165
Summary: Enables aminopeptidase activity. Involved in several processes, including neuropeptide catabolic process; positive regulation of blood pressure; and regulation of long-term neuronal synaptic plasticity. Located in several cellular components, including insulin-responsive compartment; neuronal cell body; and perinuclear region of cytoplasm. Used to study type 2 diabetes mellitus. Orthologous to human LNPEP (leucyl and cystinyl aminopeptidase). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Adrenal (RPKM 499.4), Heart (RPKM 490.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q12

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (66931712..67027610, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (58258642..58355532, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (59289392..59384540, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Acute inhibition of IRAP aminopeptidase activity does not affect glucose homeostasis.
Title: Insulin-regulated aminopeptidase inhibitors do not alter glucose handling in normal and diabetic rats.
Ang IV protects myocardial injury against reperfusion by inhibiting inflammation and apoptosis via AT4R and PI3K-Akt-mTOR signaling pathway in anesthetized rats.
Title: Angiotensin IV protects cardiac reperfusion injury by inhibiting apoptosis and inflammation via AT4R in rats.
IRAP is involved in vesicular trafficking and shares common regional distribution with the sst2A receptor in the hippocampus.
Title: Trans-Modulation of the Somatostatin Type 2A Receptor Trafficking by Insulin-Regulated Aminopeptidase Decreases Limbic Seizures.
Sol and MB CysAP activities, acting separately or in concert and mainly in renal medulla, regulate the function of their susceptible endogenous substrates, and may participate meaningfully in the control of blood pressure and fluid balance.
Title: Tissue distribution of CysAP activity and its relationship to blood pressure and water balance.
Increased calcium influx through postsynaptic calcium channels contribute to Norleucine1-angiotensin IV receptor-induced enhancement of long-term potentiation.
Title: AT4 receptor activation increases intracellular calcium influx and induces a non-N-methyl-D-aspartate dependent form of long-term potentiation.
found a decrease of hypothalamic IRAP activity and an increase in circulating levels of oxytocin
Title: Hypothalamus-pituitary-thyroid axis disruption in rats with breast cancer is related to an altered endogenous oxytocin/insulin-regulated aminopeptidase (IRAP) system.
The present study clearly demonstrated that the activities of AlaAP and CysAP in the hippocampus were associated with lateralization in normal rats.
Title: Correlations between angiotensinase activity asymmetries in the brain and paw preference in rats.
results show that acute immobilization stress affects differentially enkephalinase and oxytocinase activities depending on the fraction and brain region analyzed
Title: Stress influences brain enkephalinase, oxytocinase and angiotensinase activities: a new hypothesis.
Acyl-coa dehydrogenases are localized on GLUT4-containing vesicles via association with insulin-regulated aminopeptides (VP165) in a manner dependent on its dileucine motif
Title: Acyl-coenzyme A dehydrogenases are localized on GLUT4-containing vesicles via association with insulin-regulated aminopeptidase in a manner dependent on its dileucine motif.
IRAP vesicles represent a second IgE-sensitive exocytotic compartment in mast cells
Title: Insulin-regulated aminopeptidase marks an antigen-stimulated recycling compartment in mast cells.

Submit: New GeneRIF Correction See all GeneRIFs (14)

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables aminopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables aminopeptidase activity	IEA Inferred from Electronic Annotation more info
enables aminopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables aminopeptidase activity	ISO Inferred from Sequence Orthology more info
enables metalloaminopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptide binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in negative regulation of cold-induced thermogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cold-induced thermogenesis	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in neuropeptide catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptide catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptide catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in peptide catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of blood pressure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of blood pressure	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of long-term neuronal synaptic plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hormone	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle membrane	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in insulin-responsive compartment	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: leucyl-cystinyl aminopeptidase

Names: aminopeptidase Vp165; angiotensin IV receptor; cystinyl aminopeptidase; insulin-regulated membrane aminopeptidase IRAP; insulin-responsive aminopeptidase; leucyl-cystinyl aminopeptidase-like; leucyl/cystinyl aminopeptidase; oxytocinase; placental leucine aminopeptidase; vesicle protein of 165 kDa

NP_001106874.1

EC 3.4.11.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113403.3 → NP_001106874.1 leucyl-cystinyl aminopeptidase

See identical proteins and their annotated locations for NP_001106874.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

P97629, Q11009

UniProtKB/TrEMBL

A0A0G2JUW8, A6KB65

Related

ENSRNOP00000017718.7, ENSRNOT00000017718.8

Conserved Domains (3) summary

cd09601
Location:176 → 617

M1_APN_2; Peptidase M1 Aminopeptidase N family incudes tricorn interacting factor F3, Endoplasmic reticulum aminopeptidase 1 (ERAP1), Aminopeptidase Q (APQ)

pfam01433
Location:167 → 552

Peptidase_M1; Peptidase family M1

pfam11838
Location:690 → 1005

ERAP1_C; ERAP1-like C-terminal domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

66931712..67027610 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_133574.1: Suppressed sequence

Description

NM_133574.1: This RefSeq was removed because currently there is insufficient support for this transcript.