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waaG lipopolysaccharide glucosyltransferase I [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948149, updated on 3-Dec-2024

Summary

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Official Symbol
waaG
Official Full Name
lipopolysaccharide glucosyltransferase I
Primary source
ECOCYC:EG11339
Locus tag
b3631
See related
ASAP:ABE-0011868
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3621; rfaG
Summary
Mutants have reduced OM vesiculation (McBroom, 2006). [More information is available at EcoGene: EG11339]. The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG11339].
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Genomic context

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Sequence:
NC_000913.3 (3805943..3807067, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene UDP-D-glucose:(glucosyl)LPS alpha-1,3-glucosyltransferase Neighboring gene UDP-D-galactose:(glucosyl)lipopolysaccharide-1,6-D-galactosyltransferase Neighboring gene lipopolysaccharide core biosynthesis protein WaaS Neighboring gene lipopolysaccharide core heptose (I) kinase Neighboring gene lipopolysaccharide core heptosyltransferase 3 Neighboring gene ncRNA Neighboring gene KDO transferase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4. Martinez-Fleites C, et al. Chem Biol, 2006 Nov. PMID 17113996
  2. Cloning genes for bacterial glycosyltransferases. Creeger ES, et al. Methods Enzymol, 1982. PMID 6212748
  3. Cloning of genes for bacterial glycosyltransferases. I. Selection of hybrid plasmids carrying genes for two glucosyltransferases. Creeger ES, et al. J Biol Chem, 1979 Feb 10. PMID 368061
  4. Identification by Tn10 transposon mutagenesis of host factors involved in the biosynthesis of K99 fimbriae of Escherichia coli: effect of LPS core mutations. Pilipcinec E, et al. FEMS Microbiol Lett, 1994 Oct 15. PMID 7988890
  5. Mutation of the lipopolysaccharide core glycosyltransferase encoded by waaG destabilizes the outer membrane of Escherichia coli by interfering with core phosphorylation. Yethon JA, et al. J Bacteriol, 2000 Oct. PMID 10986272, Free PMC Article

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. biofilm mutants with altered lipopolysaccharide synthesis (waaG) exhibited increased cell-cell interactions, bypassed the polar attachment step, and produced FFT spectra characteristic of aperiodic cell distribution.
    Title: Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables glycosyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables hexosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables lipopolysaccharide glucosyltransferase I activity EXP
Inferred from Experiment
more info
 PubMed 
enables lipopolysaccharide glucosyltransferase I activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipopolysaccharide glucosyltransferase I activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in lipopolysaccharide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide core region biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lipopolysaccharide core region biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
lipopolysaccharide glucosyltransferase I

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3805943..3807067 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418088.1 lipopolysaccharide glucosyltransferase I [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418088.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5C9AFR4
    Conserved Domains (1) summary
    cd03801
    Location:3 → 368
    GT4_PimA-like; phosphatidyl-myo-inositol mannosyltransferase
Nucleotide Protein
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