U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

degS serine endoprotease [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947865, updated on 3-Dec-2024

Summary

Go to the top of the page Help
Official Symbol
degS
Official Full Name
serine endoprotease
Primary source
ECOCYC:EG11652
Locus tag
b3235
See related
ASAP:ABE-0010611
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3224; hhoB; htrH
Summary
degS is an essential gene. [More information is available at EcoGene: EG11652]. Upon extracytoplasmic stress, misfolded/unfolded porins interact with the DegS PDZ domain, releasing an inhibitory interaction and activating DegS protease . [More information is available at EcoCyc: EG11652].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (3382200..3383267)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene Z-ring associated protein G Neighboring gene periplasmic serine endoprotease Neighboring gene malate dehydrogenase Neighboring gene DNA-binding transcriptional dual regulator ArgR

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Expression, crystallization and crystallographic analysis of DegS, a stress sensor of the bacterial periplasm. Grininger M, et al. Acta Crystallogr D Biol Crystallogr, 2004 Aug. PMID 15272167
  2. Mechanisms for allosteric activation of protease DegS by ligand binding and oligomerization as revealed from molecular dynamics simulations. Lu C, et al. Proteins, 2016 Nov. PMID 27556733
  3. Structural analysis of DegS, a stress sensor of the bacterial periplasm. Zeth K. FEBS Lett, 2004 Jul 2. PMID 15225661
  4. Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease. Wilken C, et al. Cell, 2004 May 14. PMID 15137941
  5. Allosteric regulation of DegS protease subunits through a shared energy landscape. Mauldin RV, et al. Nat Chem Biol, 2013 Feb. PMID 23201899, Free PMC Article

See all (45) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The enhanced sigma E factor activity of DegS A323E substitution suppresses a number of assembly-defective conditions without exhibiting the toxicity associated with high levels of sigma E factor activity.
    Title: Fine-Tuning of σ(E) Activation Suppresses Multiple Assembly-Defective Mutations in Escherichia coli.
  2. Suppressor mutations in degS overcome the acute temperature-sensitive phenotype of DeltadegP and DeltadegP Deltatol-pal mutants of Escherichia coli.
    Title: Suppressor Mutations in degS Overcome the Acute Temperature-Sensitive Phenotype of ΔdegP and ΔdegP Δtol-pal Mutants of Escherichia coli.
  3. Mechanisms for allosteric activation of protease DegS by ligand binding and oligomerization has been described.
    Title: Mechanisms for allosteric activation of protease DegS by ligand binding and oligomerization as revealed from molecular dynamics simulations.
  4. DegS and RseB are proposed to be stress sensor proteins for sigmaE signal transduction [review]
    Title: Two stress sensor proteins for the expression of sigmaE regulon: DegS and RseB.
  5. analysis of allosteric regulation of DegS protease subunits through a shared energy landscape
    Title: Allosteric regulation of DegS protease subunits through a shared energy landscape.
  6. Allostery is an intrinsic property of the protease domain of DegS
    Title: Allostery is an intrinsic property of the protease domain of DegS: implications for enzyme function and evolution.
  7. The combined introduction of loop L2 and PDZ domain 1 of DegP into DegS converted DegS even further into a DegP-like protease.
    Title: Conversion of a regulatory into a degradative protease.
  8. after DegS cleavage, the newly exposed carboxyl terminus of RseA may facilitate Site-2 cleavage through direct interaction with the PDZ domain.
    Title: Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavage.
  9. Self-cleavage occurs when HtrA reassembles back into the hexameric structure
    Title: Characterization of the autocleavage process of the Escherichia coli HtrA protein: implications for its physiological role.
  10. Residues involved in the DegS allosteric switch are conserved in the DegP/HtrA and HtrA2/Omi families, suggesting that many PDZ proteases use a common mechanism of allosteric activation.
    Title: Allosteric activation of DegS, a stress sensor PDZ protease.
Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables endopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables peptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables serine-type endopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables serine-type endopeptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables serine-type peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables serine-type peptidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within cellular response to misfolded protein IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within proteolysis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within proteolysis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
serine endoprotease

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3382200..3383267
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417702.1 serine endoprotease [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417702.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1V2GF05
    Conserved Domains (1) summary
    PRK10898
    Location:1 → 354
    PRK10898; serine endoprotease DegS
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.