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mscL large conductance mechanosensitive channel [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947787, updated on 3-Dec-2024

Summary

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Official Symbol
mscL
Official Full Name
large conductance mechanosensitive channel
Primary source
ECOCYC:EG11180
Locus tag
b3291
See related
ASAP:ABE-0010790
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3277; yhdC
Summary
The Escherichia coli PDB structures for MscL are homology models. otherPDB (M. tuberculosis): [More information is available at EcoGene: EG11180]. MscL is the mechanosensitive channel of large conductance that is involved in the transduction of membrane stress (mechanical or osmotic) into an electrochemical response . [More information is available at EcoCyc: EG11180].
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Genomic context

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Sequence:
NC_000913.3 (3438024..3438434)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 16S rRNA m(5)C967 methyltransferase Neighboring gene NAD-binding component of Trk potassium transporters Neighboring gene alternative ribosome-rescue factor A Neighboring gene DNA-binding transcriptional activator ZntR Neighboring gene DUF1992 domain-containing protein YhdN

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Dynamical properties of the MscL of Escherichia coli: a normal mode analysis. Valadié H, et al. J Mol Biol, 2003 Sep 19. PMID 12963374
  2. Channel gating: twist to open. Biggin PC, et al. Curr Biol, 2001 May 1. PMID 11369249
  3. Structural models of the MscL gating mechanism. Sukharev S, et al. Biophys J, 2001 Aug. PMID 11463635, Free PMC Article
  4. Site-directed spin-labeling analysis of reconstituted Mscl in the closed state. Perozo E, et al. J Gen Physiol, 2001 Aug. PMID 11479346, Free PMC Article
  5. A mechanosensitive channel protein and its gene in E. coli. Blount P, et al. Gravit Space Biol Bull, 1997 Jun. PMID 11540118

See all (166) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A crayfish ALF inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli.
    Title: A crayfish ALF inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli.
  2. Interaction between mechanosensitive channels embedded in lipid membrane.
    Title: Interaction between mechanosensitive channels embedded in lipid membrane.
  3. The study described a novel class of MscL mutations that were correlated with the thermodynamic stability of the MscL structure.
    Title: Temperature-sensitive mutants of MscL mechanosensitive channel.
  4. Collectively, the findings indicate that MscL-dependent protein excretion is positively regulated in response to both osmotic stress and arfA-mediated translational stress.
    Title: Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins.
  5. Because MscL opens to tension in the lipid bilayer, requiring no other proteins or ligands, it could be developed into a general noninvasive sonogenetic tool to manipulate the activities of neurons or other cells and potential nanodevices.
    Title: Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL.
  6. the amphipathic N-terminal helix of MscL acts as a crucial structural element during tension-induced gating, both stabilizing the closed state and coupling the channel to the membrane
    Title: The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels.
  7. More importantly, the FE model indicates that to fully operate an MscL nanovalve by pulling directly on the N-terminus and bottom of TM1, gain-of-function (GOF) mutants (e.g., G22N MscL) would have to be employed rather than the WT MscL channel.
    Title: Pulling MscL open via N-terminal and TM1 helices: A computational study towards engineering an MscL nanovalve.
  8. Data suggest that dihydrostreptomycin binds to a specific site on mechanosensitive channel of large conductance (MscL) and modifies its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell.
    Title: Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore.
  9. comprehensive screen has identified many residues that are critical for channel function as well as highlighted MscL domains and residues that undergo the most drastic environmental changes upon gating.
    Title: Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations.
  10. Observe directly that the MscL channel opens via the helix-tilt model and the open pore reaches 2.8 nm in diameter.
    Title: Single molecule FRET reveals pore size and opening mechanism of a mechano-sensitive ion channel.
Submit: New GeneRIF Correction See all GeneRIFs (25)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables mechanosensitive monoatomic ion channel activity EXP
Inferred from Experiment
more info
 PubMed 
enables mechanosensitive monoatomic ion channel activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables mechanosensitive monoatomic ion channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables mechanosensitive monoatomic ion channel activity IEA
Inferred from Electronic Annotation
more info
  
enables mechanosensitive monoatomic ion channel activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within intracellular water homeostasis EXP
Inferred from Experiment
more info
 PubMed 
acts_upstream_of_or_within monoatomic ion transmembrane transport EXP
Inferred from Experiment
more info
 PubMed 
acts_upstream_of_or_within monoatomic ion transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in monoatomic ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within monoatomic ion transport IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane HDA PubMed 
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
large conductance mechanosensitive channel

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3438024..3438434
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417749.1 large conductance mechanosensitive channel [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417749.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S0FJL6
    Conserved Domains (1) summary
    PRK00567
    Location:3 → 136
    mscL; large-conductance mechanosensitive channel protein MscL
Nucleotide Protein
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