recB exodeoxyribonuclease V subunit RecB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: recB
Official Full Name: exodeoxyribonuclease V subunit RecB
Primary source: ECOCYC:EG10824
Locus tag: b2820
See related: ASAP:ABE-0009249
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2816; ior; rorA
Summary: RecBCD is a bipolar helicase. [More information is available at EcoGene: EG10824]. RecB, RecC, and RecD make up a helicase/nuclease complex that is essential for both homologous recombination during the course of transduction and conjugation as well as in repair of double-strand breaks in Escherichia coli . [More information is available at EcoCyc: EG10824].
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Genomic context

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Sequence:: NC_000913.3 (2952461..2956003, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Trans-complementation by the RecB nuclease domain of RecBCD enzyme reveals new insight into RecA loading upon chi recognition.
Title: Trans-complementation by the RecB nuclease domain of RecBCD enzyme reveals new insight into RecA loading upon χ recognition.
Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.
Title: Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.
Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair.
Title: Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair.
Data show that RecBCD and its subunits are able to generate forces up to 25-40 pN without a significant effect on their velocity.
Title: Synergy between RecBCD subunits is essential for efficient DNA unwinding.
From these results, we suppose that the tether transmits a force, discussed below, from the RecB helicase domain to the RecB nuclease domain to place the nuclease domain in the correct position to allow Chi cutting. The tether must be sufficiently flexible, but not too flexible, to transmit this force
Title: The RecB helicase-nuclease tether mediates Chi hotspot control of RecBCD enzyme.
Data show genetic analysis RecBCD and naive adaptation in Escherichia coli.
Title: CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases.
Multiple ssDNA translocases within the RecBCD complex both before and after chi ensures processive unwinding of DNA substrates required for efficient recombination events.
Title: How Chi Sequence Modifies RecBCD Single-Stranded DNA Translocase Activity.
This is the first occasion that RecBCD has been demonstrated to be inhibited by DNA adducts induced by cisplatin or UV. In addition, we quantified the amounts of DNA remaining after RecBCD treatment and observed that the level of inhibition was concentration and dose dependent. A DNA-targeted 9-aminoacridinecarboxamide cisplatin analogue was also found to inhibit RecBCD activity.
Title: RecBCD (Exonuclease V) is inhibited by DNA adducts produced by cisplatin and ultraviolet light.
Extension of the 5'-tail of the unwound duplex induces a large conformational change in the RecD subunit, that is transferred through the RecC subunit to activate the nuclease domain of the RecB subunit.
Title: Mechanism for nuclease regulation in RecBCD.
A new role of the RecBCD complex in the processing of DNA single-strand gaps that are generated at DNA replication-blocking lesions: independently of its nuclease or helicase activities, the entire RecBCD complex is required for recombinational repair of the gap and efficient translesion synthesis.
Title: A non-catalytic role of RecBCD in homology directed gap repair and translesion synthesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables DNA translocase activity	IDA Inferred from Direct Assay more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables exodeoxyribonuclease V activity	IDA Inferred from Direct Assay more info	PubMed
enables exodeoxyribonuclease V activity	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA recombination	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in clearance of foreign intracellular DNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within double-strand break repair via homologous recombination	TAS Traceable Author Statement more info	PubMed
involved_in recombinational repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in recombinational repair	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to radiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of exodeoxyribonuclease V complex	IBA Inferred from Biological aspect of Ancestor more info
part_of exodeoxyribonuclease V complex	IDA Inferred from Direct Assay more info	PubMed
part_of exodeoxyribonuclease V complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: exodeoxyribonuclease V subunit RecB

NP_417297.1

EC 3.1.11.5

EC 3.1.21.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2952461..2956003 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417297.1 exodeoxyribonuclease V subunit RecB [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417297.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A6M9X2W8

Conserved Domains (1) summary

PRK10876
Location:1 → 1180

recB; exonuclease V subunit beta; Provisional