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proB glutamate 5-kinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946425, updated on 17-Dec-2024

Summary

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Official Symbol
proB
Official Full Name
glutamate 5-kinase
Primary source
ECOCYC:EG10768
Locus tag
b0242
See related
ASAP:ABE-0000830
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0243; pro(2); pro2
Summary
Contains PUA domain, required for proline-triggered aggregation. [More information is available at EcoGene: EG10768].
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Genomic context

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Sequence:
NC_000913.3 (260388..261491)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene IS1 family transposase B Neighboring gene pseudo Neighboring gene IS1 family repressor TnpA Neighboring gene outer membrane porin PhoE Neighboring gene glutamate-5-semialdehyde dehydrogenase Neighboring gene tRNA-Thr

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Molecular mechanisms modulating glutamate kinase activity. Identification of the proline feedback inhibitor binding site. Pérez-Arellano I, et al. J Mol Biol, 2010 Dec 17. PMID 20970428
  2. Activation of glutamate by gamma-glutamate kinase: formation of gamma-cis-cycloglutamyl phosphate, an analog of gamma-glutamyl phosphate. Seddon AP, et al. J Biol Chem, 1989 Jul 5. PMID 2567735
  3. Nucleotide sequence of a mutation in the proB gene of Escherichia coli that confers proline overproduction and enhanced tolerance to osmotic stress. Csonka LN, et al. Gene, 1988 Apr 29. PMID 2841193
  4. Mapping active site residues in glutamate-5-kinase. The substrate glutamate and the feed-back inhibitor proline bind at overlapping sites. Pérez-Arellano I, et al. FEBS Lett, 2006 Nov 13. PMID 17069808
  5. Proline synthesis in Escherichia coli. A proline-inhibitable glutamic acid kinase. Baich A. Biochim Biophys Acta, 1969 Dec 30. PMID 4904678

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A synonymous point mutation in the coding region of proB creates a new promoter for proA* and this mutation increases the level of ProA* by 2-fold but increases the growth rate by 5-fold.
    Title: A Synonymous Mutation Upstream of the Gene Encoding a Weak-Link Enzyme Causes an Ultrasensitive Response in Growth Rate.
  2. The proline-binding cavity partially overlaps with the glutamate substrate site, and the interaction of both proline and glutamate with GK is modulated by a flexible, 16-residue loop linking beta-sheet 4 and alpha-helix E in the active-center cavity.
    Title: Molecular mechanisms modulating glutamate kinase activity. Identification of the proline feedback inhibitor binding site.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.
  4. G5K and NAGK closely resemble each other concerning substrate binding and catalysis, but that they have different mechanisms of feed-back control.
    Title: Mapping active site residues in glutamate-5-kinase. The substrate glutamate and the feed-back inhibitor proline bind at overlapping sites.
  5. X ray structure
    Title: Glutamate-5-kinase from Escherichia coli: gene cloning, overexpression, purification and crystallization of the recombinant enzyme and preliminary X-ray studies.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables glutamate 5-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutamate 5-kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutamate 5-kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables proline binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in L-proline biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within L-proline biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in proline biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proline biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within proline biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
glutamate 5-kinase
NP_414777.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    260388..261491
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414777.1 glutamate 5-kinase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414777.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A192CI68, A0AA36KUS5
    Conserved Domains (1) summary
    PRK05429
    Location:1 → 365
    PRK05429; gamma-glutamyl kinase; Provisional
Nucleotide Protein
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