U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

rlmL fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945564, updated on 3-Dec-2024

Summary

Go to the top of the page Help
Official Symbol
rlmL
Official Full Name
fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
Primary source
ECOCYC:G6488
Locus tag
b0948
See related
ASAP:ABE-0003211
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0939; rlmK; rlmKL; ycbY
Summary
rlmL is a non-essential gene co-transcribed with downstream uup gene. [More information is available at EcoGene: EG13717]. RlmL is the methyltransferase responsible for methylation of 23S rRNA at the N2 position of the G2445 nucleotide. [More information is available at EcoCyc: G6488].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (1007844..1009952)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene dihydroorotate dehydrogenase, type 2 Neighboring gene cell division protein ZapC Neighboring gene 6-N-hydroxylaminopurine resistance protein Neighboring gene ATP-binding protein Uup Neighboring gene intermembrane transport protein PqiA

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA. Wang KT, et al. Nucleic Acids Res, 2012 Jun. PMID 22362734, Free PMC Article
  2. Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity. Kimura S, et al. Nucleic Acids Res, 2012 May. PMID 22210896, Free PMC Article
  3. Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m(2)G2445 methyltransferase RlmL from Escherichia coli. Wang KT, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2010 Nov 1. PMID 21045301, Free PMC Article
  4. An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors. Toh SM, et al. J Mol Biol, 2008 Jul 18. PMID 18554609, Free PMC Article
  5. Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli. Reddy M, et al. J Bacteriol, 2000 Apr. PMID 10715006, Free PMC Article

See all (16) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. E. coli YcbY is a bifunctional rRNA methyltransferase catalyzing the m2G2445 and m7G2069 modifications located on opposite sides of helix 74.
    Title: Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA.
  2. RlmKL is a novel type of fused methyltransferase bearing dual active sites responsible for different methyl modifications at distinct positions.
    Title: Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.
  3. final crystals of RlmL diffracted to 2.2 A resolution
    Title: Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m(2)G2445 methyltransferase RlmL from Escherichia coli.
  4. Results describe the identification of a gene, ycbY, as encoding an enzyme responsible for methylation of 23 S rRNA G2445.
    Title: Identification of Escherichia coli m2G methyltransferases: I. the ycbY gene encodes a methyltransferase specific for G2445 of the 23 S rRNA.
Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 23S rRNA (guanine(2445)-N(2))-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rRNA (guanine-N2-)-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (guanine-N2-)-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables rRNA (guanine-N2-)-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (guanine-N7-)-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (guanine-N7-)-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables rRNA (guanine-N7-)-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA (guanine-N7)-methylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within rRNA base methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA methylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in rRNA methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IC
Inferred by Curator
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
NP_415468.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1007844..1009952
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415468.1 fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415468.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7QTG2
    Conserved Domains (1) summary
    PRK11783
    Location:1 → 702
    rlmL; bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.