Zfp423 zinc finger protein 423 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Zfp423provided by MGI
Official Full Name: zinc finger protein 423provided by MGI
Primary source: MGI:MGI:1891217
See related: Ensembl:ENSMUSG00000045333 AllianceGenome:MGI:1891217
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Roaz; Ebfaz; nur12; Zfp104; Znf423; ataxia1; mKIAA0760
Summary: Enables sequence-specific DNA binding activity. Involved in several processes, including brown fat cell differentiation; negative regulation of cold-induced thermogenesis; and positive regulation of BMP signaling pathway. Acts upstream of or within several processes, including cerebellar granule cell precursor proliferation; protein localization to cilium; and smoothened signaling pathway. Located in nucleus. Is expressed in several structures, including alimentary system; central nervous system; embryo ectoderm; genitourinary system; and sensory organ. Used to study Dandy-Walker syndrome and cerebellar disease. Human ortholog(s) of this gene implicated in nephronophthisis 14. Orthologous to human ZNF423 (zinc finger protein 423). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in whole brain E14.5 (RPKM 14.4), subcutaneous fat pad adult (RPKM 12.1) and 20 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C3; 8 42.29 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (88388438..88688665, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (87661810..87962053, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thyroid hormones regulate Zfp423 expression in regionally distinct adipose depots through direct and cell-autonomous action.
Title: Thyroid hormones regulate Zfp423 expression in regionally distinct adipose depots through direct and cell-autonomous action.
ZFP423 controls EBF2 coactivator recruitment and PPARgamma occupancy to determine the thermogenic plasticity of adipocytes.
Title: ZFP423 controls EBF2 coactivator recruitment and PPARγ occupancy to determine the thermogenic plasticity of adipocytes.
ZFP423 regulates early patterning and multiciliogenesis in the hindbrain choroid plexus.
Title: ZFP423 regulates early patterning and multiciliogenesis in the hindbrain choroid plexus.
Tight regulation of Zfp423 expression is essential for normal progression of muscle progenitors from proliferation to differentiation.
Title: Zfp423 Regulates Skeletal Muscle Regeneration and Proliferation.
In vivo, vitamin A supplementation prevents obesity, downregulates Gadd45a expression, and reduces GADD45A binding and DNA demethylation in the Zfp423 promoter.
Title: Retinoic acid inhibits white adipogenesis by disrupting GADD45A-mediated Zfp423 DNA demethylation.
ZFP423 to be expressed in the developing cortex and promote RA-dependent neuronal differentiation.
Title: TBR2 antagonizes retinoic acid dependent neuronal differentiation by repressing Zfp423 during corticogenesis.
Zfp423/ZNF423 has a role in regulating cell cycle progression, the mode of cell division and the DNA-damage response in Purkinje neuron progenitors
Title: Zfp423/ZNF423 regulates cell cycle progression, the mode of cell division and the DNA-damage response in Purkinje neuron progenitors.
These data identify Zfp423 as a molecular brake on adipocyte thermogenesis.
Title: Zfp423 Maintains White Adipocyte Identity through Suppression of the Beige Cell Thermogenic Gene Program.
loss of Zfp423 alters expression of several genes encoding key cilium components, including increased expression of Tulp3. Tulp3 is a direct binding target of Zfp423 and reducing the overexpression of Tulp3 in Zfp423-deficient cells suppresses Smoothened translocation defects.
Title: Zfp423 Regulates Sonic Hedgehog Signaling via Primary Cilium Function.
Data show that the expression of miR-195a is regulated during adipocyte differentiation and identified Zfp423 as its novel target.
Title: miR-195a inhibits adipocyte differentiation by targeting the preadipogenic determinator Zfp423.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (14) 1 citation
Gene trapped (3) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D8Mit57 (e-PCR), detects polymorphism
- UniSTS:131600

Zfp423 (e-PCR), detects polymorphism
- UniSTS:474696

D8Mit208 (e-PCR), detects polymorphism
- UniSTS:131416

STS-Z41302 (e-PCR)
- UniSTS:74207

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info	PubMed

Process	Evidence Code	Pubs
involved_in Notch signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in brown fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cerebellar granule cell precursor proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cold-induced thermogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of BMP signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein localization to cilium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization to cilium	ISO Inferred from Sequence Orthology more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in white fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: zinc finger protein 423

Names: early B-cell factor associated zinc finger protein; early B-cell factor associated zinc finger transcription factor; olf1/EBF-associated zinc finger protein; smad- and Olf-interacting zinc finger protein; zinc finger protein 104

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001310520.1 → NP_001297449.1 zinc finger protein 423 isoform 2

See identical proteins and their annotated locations for NP_001297449.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus than isoform 1.

Source sequence(s)

AI845255, AK133497, BC079586

Consensus CDS

CCDS80911.1

UniProtKB/TrEMBL

G3UW89, Q3V017

Related

ENSMUSP00000129724.3, ENSMUST00000165770.9

Conserved Domains (2) summary

COG5048
Location:77 → 478

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:23 → 43

ZF_C2H2; C2H2 Zn finger [structural motif]
NM_033327.2 → NP_201584.2 zinc finger protein 423 isoform 1

See identical proteins and their annotated locations for NP_201584.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK122365, BB788751, BF021428, BQ180174, BU519630

Consensus CDS

CCDS52627.1

UniProtKB/Swiss-Prot

B2RSW4, Q6PCP2, Q6X497, Q80TS5, Q8CIQ1, Q9ESD2

UniProtKB/TrEMBL

Q3V017

Related

ENSMUSP00000105282.3, ENSMUST00000109655.9

Conserved Domains (2) summary

COG5048
Location:202 → 603

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:148 → 168

ZF_C2H2; C2H2 Zn finger [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000074.7 Reference GRCm39 C57BL/6J

Range

88388438..88688665 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030243876.2 → XP_030099736.1 zinc finger protein 423 isoform X3

UniProtKB/TrEMBL

Q3V017

Related

ENSMUSP00000052379.9, ENSMUST00000052250.15

Conserved Domains (2) summary

COG5048
Location:192 → 593

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:138 → 158

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_011248560.3 → XP_011246862.1 zinc finger protein 423 isoform X1

UniProtKB/TrEMBL

Q3V017

Conserved Domains (2) summary

COG5048
Location:222 → 623

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:168 → 188

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_017313033.2 → XP_017168522.1 zinc finger protein 423 isoform X2

UniProtKB/TrEMBL

Q3V017

Conserved Domains (2) summary

COG5048
Location:205 → 606

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:151 → 171

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_030243878.1 → XP_030099738.1 zinc finger protein 423 isoform X4

UniProtKB/TrEMBL

G3UW89, Q3V017

Conserved Domains (2) summary

COG5048
Location:77 → 478

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:23 → 43

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_006531539.4 → XP_006531602.1 zinc finger protein 423 isoform X4

See identical proteins and their annotated locations for XP_006531602.1

UniProtKB/TrEMBL

G3UW89, Q3V017

Conserved Domains (2) summary

COG5048
Location:77 → 478

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:23 → 43

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_030243879.1 → XP_030099739.1 zinc finger protein 423 isoform X4

UniProtKB/TrEMBL

G3UW89, Q3V017

Conserved Domains (2) summary

COG5048
Location:77 → 478

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:23 → 43

ZF_C2H2; C2H2 Zn finger [structural motif]
XM_011248561.4 → XP_011246863.1 zinc finger protein 423 isoform X4

See identical proteins and their annotated locations for XP_011246863.1

UniProtKB/TrEMBL

G3UW89, Q3V017

Conserved Domains (2) summary

COG5048
Location:77 → 478

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:23 → 43

ZF_C2H2; C2H2 Zn finger [structural motif]