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NEM1 Nem1-Spo7 phosphatase catalytic subunit NEM1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856393, updated on 4-Jan-2025

Summary

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Official Symbol
NEM1
Official Full Name
Nem1-Spo7 phosphatase catalytic subunit NEM1
Primary source
SGD:S000001046
Locus tag
YHR004C
See related
AllianceGenome:SGD:S000001046; FungiDB:YHR004C; VEuPathDB:YHR004C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Contributes to phosphoprotein phosphatase activity. Involved in lipid droplet formation; nuclear envelope organization; and regulation of lipid biosynthetic process. Located in endoplasmic reticulum; lipid droplet; and membrane. Part of Nem1-Spo7 phosphatase complex. Orthologous to human CTDNEP1 (CTD nuclear envelope phosphatase 1). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See NEM1 in Genome Data Viewer
Location:
chromosome: VIII
Exon count:
1
Sequence:
Chromosome: VIII; NC_001140.6 (111754..113094, complement)

Chromosome VIII - NC_001140.6Genomic Context describing neighboring genes Neighboring gene coenzyme A transporter Neighboring gene tRNA threonylcarbamoyladenosine dehydratase Neighboring gene guanine nucleotide-binding protein subunit alpha Neighboring gene protein transporter TIM10

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A putative NEM1 homologue regulates lipid droplet biogenesis via PAH1 in Tetrahymena thermophila. Shukla S, et al. J Biosci, 2018 Sep. PMID 30207315
  2. Catalytic core function of yeast Pah1 phosphatidate phosphatase reveals structural insight into its membrane localization and activity control. Han GS, et al. J Biol Chem, 2024 Jan. PMID 38097185, Free PMC Article
  3. NEM1 acts as a suppressor of apoptotic phenotypes in LSM4 yeast mutants. Palermo V, et al. FEMS Yeast Res, 2015 Nov. PMID 26316593
  4. Proteomics-Based Monitoring of Pathway Activity Reveals that Blocking Diacylglycerol Biosynthesis Rescues from Alpha-Synuclein Toxicity. Soste M, et al. Cell Syst, 2019 Sep 25. PMID 31521608, Free PMC Article
  5. Conserved regions of the regulatory subunit Spo7 are required for Nem1-Spo7/Pah1 phosphatase cascade function in yeast lipid synthesis. Jog R, et al. J Biol Chem, 2023 May. PMID 37030502, Free PMC Article

See all (89) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Phosphorylation-mediated regulation of the Nem1-Spo7/Pah1 phosphatase cascade in yeast lipid synthesis.
    Title: Phosphorylation-mediated regulation of the Nem1-Spo7/Pah1 phosphatase cascade in yeast lipid synthesis.
  2. Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis.
    Title: Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis.
  3. The Spo7 sequence LLI is required for Nem1-Spo7/Pah1 phosphatase cascade function in yeast lipid metabolism.
    Title: The Spo7 sequence LLI is required for Nem1-Spo7/Pah1 phosphatase cascade function in yeast lipid metabolism.
  4. the TORC1-Nem1/Spo7-Pah1 axis is a master regulator of not only macroautophagy but also microautophagy in budding yeast
    Title: The TORC1-Nem1/Spo7-Pah1/lipin axis regulates microautophagy induction in budding yeast.
  5. Together, our data suggest that Nem1-Sop7-mediated regulation of membrane biogenesis is needed to promote mitophagy in yeast.
    Title: The Nem1-Spo7 protein phosphatase complex is required for efficient mitophagy in yeast.
  6. Nem1p regulates nuclear growth by controlling phospholipid biosynthesis and it is required for normal nuclear envelope morphology and sporulation.
    Title: NEM1 acts as a suppressor of apoptotic phenotypes in LSM4 yeast mutants.
  7. Acidic tail-dependent binding and dephosphorylation of Pah1p by the Nem1p-Spo7p complex is an important determinant of its function in lipid and membrane biogenesis.
    Title: Regulation of lipid droplet and membrane biogenesis by the acidic tail of the phosphatidate phosphatase Pah1p.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables MAP kinase serine/threonine phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables calmodulin-dependent protein phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables histone H2AXS140 phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables myosin phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to phosphoprotein phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphoprotein phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in chromatin remodeling IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid droplet formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of phospholipid biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in nuclear envelope organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of lipid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of phospholipid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of Nem1-Spo7 phosphatase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in lipid droplet IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion HDA PubMed 
located_in nuclear membrane IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Nem1-Spo7 phosphatase catalytic subunit NEM1
NP_011867.1
  • Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear/ER membrane association and activation of Pah1p, a phosphatidate phosphatase involved in the production of diacylglycerol DAG for lipid droplet biogenesis; regulates nuclear growth by controlling phospholipid biosynthesis; required for normal nuclear envelope morphology and sporulation; phosphatase activity of the Nem1p-Spo7p complex is inhibited by Ice2p; homolog of the human protein Dullard

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001140.6 Reference assembly

    Range
    111754..113094 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179134.1 → NP_011867.1  TPA: Nem1-Spo7 phosphatase catalytic subunit NEM1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011867.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D3DKU7, P38757
    UniProtKB/TrEMBL
    B3LSC2, B5VJT0, C8ZA70
    Conserved Domains (1) summary
    COG5190
    Location:9 → 446
    FCP1; TFIIF-interacting CTD phosphatase, includes NLI-interacting factor [Transcription]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38757.1

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