YKU70 ATP-dependent DNA helicase YKU70 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: YKU70
Official Full Name: ATP-dependent DNA helicase YKU70
Primary source: SGD:S000004897
Locus tag: YMR284W
See related: AllianceGenome:SGD:S000004897; FungiDB:YMR284W; VEuPathDB:YMR284W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HDF1; NES24
Summary: Enables telomerase RNA binding activity. Involved in several processes, including constitutive heterochromatin formation; double-strand break repair; and establishment of protein-containing complex localization to telomere. Located in nuclear envelope. Part of Ku70:Ku80 complex. Orthologous to human XRCC6 (X-ray repair cross complementing 6). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See YKU70 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (838187..839995)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.
Title: Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.
Ku complex prevents meiotic recombination in the elimination of MRX activity.
Title: Ku complex suppresses recombination in the absence of MRX activity during budding yeast meiosis.
DNA with ends occluded by the DNA end-joining factor Ku70-Ku80 becomes a suitable substrate for long-range 5'-3' resection when a nick is introduced at a locale proximal to one of the Ku-bound DNA ends.
Title: A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1-Dna2 in long-range DNA end resection.
Repair of UV-induced DNA lesions in Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.
Title: Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.
the yKu-distal portion of telomeres is bound by Rap1, which in turn reduces the potential for yKu(Yku80 and Yku70 )to mediate NHEJ. These results thus propose a solution to a long-standing conundrum, namely how to accommodate the apparently conflicting functions of Ku on telomeres.
Title: Ku Binding on Telomeres Occurs at Sites Distal from the Physical Chromosome Ends.
We found that the yku70-R456E mutant strain, which has markedly reduced association of Ku at telomeres, maintained WT levels of TLC1 nuclear localization.
Title: The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.
Yku70 sumoylation upon DNA association strengthens Ku-DNA interaction to promote multiple functions of Ku
Title: Regulation of Ku-DNA association by Yku70 C-terminal tail and SUMO modification.
The TORC1 signal is transduced by the Gln3/Gat1/Ure2 pathway, which controls the levels of the Ku heterodimer, a telomere regulator.
Title: Tor complex 1 controls telomere length by affecting the level of Ku.
Ku must load directly onto the chromosome end in order to mediate its telomeric functions
Title: Ku must load directly onto the chromosome end in order to mediate its telomeric functions.
propose that Mre11 nuclease- and Sae2-dependent DNA end processing, which initiates DSB resection prevents Ku from engaging DSBs, thus promoting Exo1-dependent resection
Title: Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damage.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables telomerase RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables telomeric DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in double-strand break repair via break-induced replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein-containing complex localization to telomere	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info
involved_in telomere maintenance	IEA Inferred from Electronic Annotation more info
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Ku70:Ku80 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Ku70:Ku80 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Ku70:Ku80 complex	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ATP-dependent DNA helicase YKU70

NP_014011.1

EC 3.6.4.12

Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

838187..839995

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182791.1 → NP_014011.1 TPA: ATP-dependent DNA helicase YKU70 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014011.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0B1, P32498, P32807, Q0P778, Q0P779, Q0P787, Q0P789, Q6B267

UniProtKB/TrEMBL

B3LMH2, B5VQ51, C7GTL5, C8ZFF1, E9P8N2, N1P0Q2

Conserved Domains (2) summary

cd00788
Location:265 → 553

KU70; Ku-core domain, Ku70 subfamily; Ku70 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. The mechanism underlying the ...

pfam03731
Location:31 → 267

Ku_N; Ku70/Ku80 N-terminal alpha/beta domain