HOT1 Hot1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: HOT1
Official Full Name: Hot1p
Primary source: SGD:S000004783
Locus tag: YMR172W
See related: AllianceGenome:SGD:S000004783; FungiDB:YMR172W; VEuPathDB:YMR172W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II general transcription initiation factor activity. Involved in RNA polymerase II preinitiation complex assembly; cellular response to osmotic stress; and positive regulation of transcription by RNA polymerase II. Located in nucleus. [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See HOT1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (605981..608140)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results presented in this work demonstrate that Hot1 enrichment is not detected through the coding regions of its target genes and rule out a direct role in transcription elongation
Title: Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes.
Hot1 is essential for transcription of just one gene, STL1. Hot1 may represent a class of transcription factors that are essential for transcription of a very few genes or even just one.
Title: The yeast Hot1 transcription factor is critical for activating a single target gene, STL1.
the C-terminal region, which corresponds to amino acids 610-719, is the DNA-binding domain of Hot1, which recognizes sequence 5'-GGGACAAA-3' located in the promoter of gene STL1. Similar sequences are found in the promoters of other Hot1 targets.
Title: The C-terminal region of the Hot1 transcription factor binds GGGACAAA-related sequences in the promoter of its target genes.
Single-cell gene expression analysis reveals altered activation of Hot1-targeted STL1 in ck2 mutants, resulting in a bimodal to unimodal shift in expression.
Title: Casein kinase II regulation of the Hot1 transcription factor promotes stochastic gene expression.
Expression of Yhr087wp under high glucose concentration is controlled by Hot1p and Sko1p transcription factors, which bind to its promoter.
Title: The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress.
effect of SCH9 on HOT1 stimulated recombination
Title: SCH9, a putative protein kinase from Saccharomyces cerevisiae, affects HOT1-stimulated recombination.
HOT1 recombination phenotypes observed in deg1 mutants are caused by Pus3p's ability to modulate frameshifting and readthrough events during translation
Title: DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II general transcription initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II general transcription initiation factor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA polymerase II preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in RNA polymerase II preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to osmotic stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of ribosomal protein gene transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Hot1p

NP_013895.1

Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

605981..608140

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182677.1 → NP_013895.1 TPA: Hot1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013895.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZZ4, Q03213

UniProtKB/TrEMBL

B3LM59, C8ZF32, N1P5Q3

Conserved Domains (2) summary

COG5422
Location:242 → 437

ROM1; RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms]

pfam12550
Location:617 → 694

GCR1_C; Transcriptional activator of glycolytic enzymes