MYO5 myosin 5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MYO5
Official Full Name: myosin 5
Primary source: SGD:S000004715
Locus tag: YMR109W
See related: AllianceGenome:SGD:S000004715; FungiDB:YMR109W; VEuPathDB:YMR109W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables microfilament motor activity. Involved in several processes, including actin cortical patch localization; positive regulation of Arp2/3 complex-mediated actin nucleation; and response to salt stress. Acts upstream of or within reticulophagy. Located in actin cortical patch. Human ortholog(s) of this gene implicated in focal segmental glomerulosclerosis 6. Orthologous to human MYO1E (myosin IE) and MYO1F (myosin IF). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See MYO5 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (486587..490246)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endocytic myosin-1 is a force-insensitive, power-generating motor.
Title: Endocytic myosin-1 is a force-insensitive, power-generating motor.
By mooring actin assembly factors to the plasma membrane, MYO5 organizes endocytic actin networks and couples actin-generated forces to the plasma membrane to drive invagination and scission. Through this unexpected mechanism, myosin facilitates force generation independent of its motor activity.
Title: Type I myosins anchor actin assembly to the plasma membrane during clathrin-mediated endocytosis.
The authors show that the type-I myosins Myo3 and Myo5 promote, in a dose-dependent way, the growth and expansion of the actin network, which controls the speed of membrane and coat internalization.
Title: Type-I myosins promote actin polymerization to drive membrane bending in endocytosis.
Concomitantly, calmodulin dissociation triggered Myo5 binding to Vrp1, extended the myosin-I lifespan at endocytic sites and activated Myo5-induced actin polymerization.
Title: Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.
Myo5 facilitates the membrane fusion/vesicle scission event of endocytosis.
Title: Dynamics of yeast Myosin I: evidence for a possible role in scission of endocytic vesicles.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables microfilament motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microfilament motor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cortical patch localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cortical patch localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in actin cortical patch localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in bipolar cellular bud site selection	TAS Traceable Author Statement more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocytosis	TAS Traceable Author Statement more info	PubMed
involved_in fungal-type cell wall organization	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in receptor-mediated endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to osmotic stress	TAS Traceable Author Statement more info	PubMed
involved_in response to salt stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to salt stress	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within reticulophagy	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within reticulophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in actin cortical patch	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cell periphery	HDA more info	PubMed
is_active_in cell tip	IBA Inferred from Biological aspect of Ancestor more info
located_in cellular bud	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in mating projection tip	HDA more info	PubMed
part_of myosin complex	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: myosin 5

NP_013827.1

One of two type I myosin motors; involved in clathrin-mediated endocytosis; activation by TEDS site phosphorylation increases actin gliding, motor activity and ligand-induced endocytosis; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

486587..490246

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182609.1 → NP_013827.1 TPA: myosin 5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013827.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZMG6, D6VZT2, Q04439

UniProtKB/TrEMBL

B5VPN4, C8ZEW2, N1P5J7

Conserved Domains (1) summary

COG5022
Location:1 → 1186

COG5022; Myosin heavy chain [General function prediction only]