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NTG1 bifunctional N-glycosylase/AP lyase NTG1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851218, updated on 4-Jan-2025

Summary

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Official Symbol
NTG1
Official Full Name
bifunctional N-glycosylase/AP lyase NTG1
Primary source
SGD:S000000013
Locus tag
YAL015C
See related
AllianceGenome:SGD:S000000013; FungiDB:YAL015C; VEuPathDB:YAL015C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
FUN33; ogg2; SCR1
Summary
Enables DNA-(apurinic or apyrimidinic site) endonuclease activity; oxidized purine nucleobase lesion DNA N-glycosylase activity; and oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity. Involved in base-excision repair, AP site formation; cellular response to oxidative stress; and positive regulation of mitochondrial DNA replication. Located in mitochondrion and nucleus. Human ortholog(s) of this gene implicated in familial adenomatous polyposis 3. Orthologous to human NTHL1 (nth like DNA glycosylase 1). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See NTG1 in Genome Data Viewer
Location:
chromosome: I
Exon count:
1
Sequence:
Chromosome: I; NC_001133.9 (126903..128102, complement)

Chromosome I - NC_001133.9Genomic Context describing neighboring genes Neighboring gene serine/threonine protein kinase PSK1 Neighboring gene protein phosphatase 2A structural subunit TPD3 Neighboring gene syntaxin Neighboring gene Rpd3L histone deacetylase complex subunit DEP1

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Regulation of base excision repair: Ntg1 nuclear and mitochondrial dynamic localization in response to genotoxic stress. Swartzlander DB, et al. Nucleic Acids Res, 2010 Jul. PMID 20194111, Free PMC Article
  2. Base excision of oxidative purine and pyrimidine DNA damage in Saccharomyces cerevisiae by a DNA glycosylase with sequence similarity to endonuclease III from Escherichia coli. Eide L, et al. Proc Natl Acad Sci U S A, 1996 Oct 1. PMID 8855249, Free PMC Article
  3. Inactivation of Saccharomyces cerevisiae OGG1 DNA repair gene leads to an increased frequency of mitochondrial mutants. Singh KK, et al. Nucleic Acids Res, 2001 Mar 15. PMID 11239005, Free PMC Article
  4. Efficient removal of formamidopyrimidines by 8-oxoguanine glycosylases. Krishnamurthy N, et al. Biochemistry, 2008 Jan 22. PMID 18154319, Free PMC Article
  5. A novel cis-acting element required for DNA damage-inducible expression of yeast DIN7. Yoshitani A, et al. Biochem Biophys Res Commun, 2008 Jan 4. PMID 17988640

See all (92) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Both Ntg1 and its human homologue, NTHL1, can be SUMO-modified in response to oxidative stress.
    Title: Identification of SUMO modification sites in the base excision repair protein, Ntg1.
  2. the elements necessary for nuclear and mitochondrial localization of Ntg1 were identified, including a bipartite classical nuclear localization signal, a mitochondrial matrix targeting sequence and the classical nuclear protein import machinery.
    Title: Regulation of base excision repair: Ntg1 nuclear and mitochondrial dynamic localization in response to genotoxic stress.
  3. The intracellular localization of the Saccharomyces cerevisiae N-glycosylase/AP lyases, Ntg1 and Ntg2, in response to nuclear and mitochondrial oxidative stress, was determined.
    Title: Dynamic compartmentalization of base excision repair proteins in response to nuclear and mitochondrial oxidative stress.
  4. The superb activity of the 8-oxoguanine glycosylases Fpg, hOGG1, and Ntg1 toward removal of FapyG and FapyA in vitro suggests that these enzymes may act upon these oxidized lesions in vivo.
    Title: Efficient removal of formamidopyrimidines by 8-oxoguanine glycosylases.
  5. loss of Ntg1p suppresses mitochondrial point mutation rates, frameshifts and recombination rates
    Title: Ntg1p, the base excision repair protein, generates mutagenic intermediates in yeast mitochondrial DNA.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA N-glycosylase activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables class I DNA-(apurinic or apyrimidinic site) endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidized base lesion DNA N-glycosylase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidized purine nucleobase lesion DNA N-glycosylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in base-excision repair IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair, AP site formation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in base-excision repair, AP site formation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in base-excision repair, AP site formation IEA
Inferred from Electronic Annotation
more info
  
involved_in cellular response to oxidative stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nucleotide-excision repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of mitochondrial DNA replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in mitochondrion HDA PubMed 
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
bifunctional N-glycosylase/AP lyase NTG1
NP_009387.1
  • DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001133.9 Reference assembly

    Range
    126903..128102 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178160.1 → NP_009387.1  TPA: bifunctional N-glycosylase/AP lyase NTG1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009387.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VPK3, P31378
    UniProtKB/TrEMBL
    A7A0F4, B5VDK8, C7GPM0, C8Z3L5, G2W8L7, N1P7K4
    Conserved Domains (1) summary
    COG0177
    Location:140 → 318
    Nth; Endonuclease III [Replication, recombination and repair]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P31378.1

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