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VAC14 Vac14p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851102, updated on 4-Jan-2025

Summary

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Official Symbol
VAC14
Official Full Name
Vac14p
Primary source
SGD:S000004378
Locus tag
YLR386W
See related
AllianceGenome:SGD:S000004378; FungiDB:YLR386W; VEuPathDB:YLR386W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables protein-macromolecule adaptor activity. Involved in several processes, including cellular hyperosmotic response; phosphatidylinositol biosynthetic process; and protein localization to vacuolar membrane. Located in fungal-type vacuole membrane. Part of PAS complex. Human ortholog(s) of this gene implicated in striatonigral degeneration. Orthologous to human VAC14 (VAC14 component of PIKFYVE complex). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See VAC14 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (893628..896270)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Elongator subunit IKI3 Neighboring gene Swc7p Neighboring gene Reh1p Neighboring gene ribosomal 40S subunit protein S29A

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Activity-dependent PI(3,5)P2 synthesis controls AMPA receptor trafficking during synaptic depression. McCartney AJ, et al. Proc Natl Acad Sci U S A, 2014 Nov 11. PMID 25355904, Free PMC Article
  2. Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation. Ho CY, et al. J Vis Exp, 2016 Jan 6. PMID 26780479, Free PMC Article
  3. Assembly of a Fab1 phosphoinositide kinase signaling complex requires the Fig4 phosphoinositide phosphatase. Botelho RJ, et al. Mol Biol Cell, 2008 Oct. PMID 18653468, Free PMC Article
  4. Vac7p, a novel vacuolar protein, is required for normal vacuole inheritance and morphology. Bonangelino CJ, et al. Mol Cell Biol, 1997 Dec. PMID 9372916, Free PMC Article
  5. Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function. Alghamdi TA, et al. J Biol Chem, 2013 Mar 29. PMID 23389034, Free PMC Article

See all (99) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct
    Title: Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function.
  2. The authors show that Vac14 exerts an effect as a scaffold for the PI(3,5)P2 regulatory complex by direct contact with the known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18.
    Title: VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse.
  3. Vac14p-Fig4p complex controls the hyperosmotic shock-induced increase in phosphatidylinositol 3,5-diphosphate levels.
    Title: The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables protein-macromolecule adaptor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-macromolecule adaptor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in cellular hyperosmotic response IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in cellular hyperosmotic response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phosphatidylinositol biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in phosphatidylinositol biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphatidylinositol biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in phosphatidylinositol biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of phosphorylation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of phosphorylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein localization to vacuolar membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of PAS complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of PAS complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of PAS complex IEA
Inferred from Electronic Annotation
more info
  
part_of PAS complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in endosome membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in fungal-type vacuole membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in fungal-type vacuole membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuolar membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Vac14p
NP_013490.3
  • Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    893628..896270
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182275.3 → NP_013490.3  TPA: Vac14p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013490.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VZ21, Q06708
    UniProtKB/TrEMBL
    B5VNS3, C7GXJ0, C8ZDZ5, N1P7A8
    Conserved Domains (3) summary
    sd00044
    Location:87 → 115
    HEAT; HEAT repeat [structural motif]
    pfam11916
    Location:573 → 746
    Vac14_Fig4_bd; Vacuolar protein 14 C-terminal Fig4p binding
    pfam12755
    Location:59 → 156
    Vac14_Fab1_bd; Vacuolar 14 Fab1-binding region
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q06708.1

Gene LinkOut

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