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NIFK nucleolar protein interacting with the FHA domain of MKI67 [ Homo sapiens (human) ]

Gene ID: 84365, updated on 27-Nov-2024

Summary

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Official Symbol
NIFKprovided by HGNC
Official Full Name
nucleolar protein interacting with the FHA domain of MKI67provided by HGNC
Primary source
HGNC:HGNC:17838
See related
Ensembl:ENSG00000155438 MIM:611970; AllianceGenome:HGNC:17838
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
Nopp34; MKI67IP
Summary
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]
Expression
Ubiquitous expression in lymph node (RPKM 19.8), appendix (RPKM 17.9) and 25 other tissues See more
Orthologs
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Genomic context

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See NIFK in Genome Data Viewer
Location:
2q14.3
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (121726945..121736875, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (122162220..122172150, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (122484521..122494451, complement)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene NIFK antisense RNA 1 Neighboring gene uncharacterized LOC124906186 Neighboring gene nucleophosmin 1 pseudogene 32 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16471 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11923 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16472 Neighboring gene translin Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:122533206-122534180 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:122534181-122535155 Neighboring gene uncharacterized LOC105373590 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:122547721-122548382

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates. Tan L, et al. Medicine (Baltimore), 2023 Oct 6. PMID 37800782, Free PMC Article
  2. The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression. Pan WA, et al. RNA Biol, 2015. PMID 25826659, Free PMC Article
  3. Direct observations of shifts in the β-sheet register of a protein-peptide complex using explicit solvent simulations. Panteva MT, et al. Biophys J, 2011 May 4. PMID 21539773, Free PMC Article
  4. Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions. Li H, et al. J Mol Biol, 2004 Jan 2. PMID 14659764
  5. Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67. Byeon IJ, et al. Nat Struct Mol Biol, 2005 Nov. PMID 16244663

See all (123) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.
    Title: NIFK as a potential prognostic biomarker in colorectal cancer correlating with immune infiltrates.
  2. NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation.
    Title: The nucleolar protein NIFK promotes cancer progression via CK1α/β-catenin in metastasis and Ki-67-dependent cell proliferation.
  3. The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
    Title: The RNA recognition motif of NIFK is required for rRNA maturation during cell cycle progression.
  4. Potential encounter complexes of the Ki67FHA receptor and hNIFK peptide are misregistered states of the beta-sheet.
    Title: Direct observations of shifts in the β-sheet register of a protein-peptide complex using explicit solvent simulations.
  5. nmr analysis of the solution structure of the FHA domain of human Ki67 and mapping of the binding surface for NIFK binding
    Title: Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify Nopp34 associated HIV-1 Gag in 293T cells PubMed
retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP) at amino acid residues 188-189 by the HIV-1 protease PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables RNA binding HDA PubMed 
enables RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in protein-containing complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in rRNA metabolic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in rRNA transcription NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in chromosome IDA
Inferred from Direct Assay
more info
  
located_in condensed nuclear chromosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
MKI67 FHA domain-interacting nucleolar phosphoprotein
Names
hNIFK
nucleolar phosphoprotein Nopp34
nucleolar protein interacting with the FHA domain of pKi-67

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_032390.5 → NP_115766.3  MKI67 FHA domain-interacting nucleolar phosphoprotein

    See identical proteins and their annotated locations for NP_115766.3

    Status: REVIEWED

    Source sequence(s)
    BC022990, BC024238, BE671589
    Consensus CDS
    CCDS2135.1
    UniProtKB/Swiss-Prot
    A8K788, Q8TB66, Q96ED4, Q9BYG3
    Related
    ENSP00000285814.4, ENST00000285814.9
    Conserved Domains (3) summary
    cd12307
    Location:46 → 119
    RRM_NIFK_like; RNA recognition motif in nucleolar protein interacting with the FHA domain of pKI-67 (NIFK) and similar proteins
    pfam12196
    Location:227 → 266
    hNIFK_binding; FHA Ki67 binding domain of hNIFK
    cl25887
    Location:2 → 151
    RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

    Range
    121726945..121736875 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060926.1 Alternate T2T-CHM13v2.0

    Range
    122162220..122172150 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9BYG3.1 GenPept UniProtKB/Swiss-Prot:Q9BYG3

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