THI1 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: THI1
Official Full Name: thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
Primary source: TAIR:AT5G54770
Locus tag: AT5G54770
See related: Araport:AT5G54770
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: MBG8.3; MBG8_3; THI1 PROTEIN; THI4; THIAMINE4; THIAZOLE REQUIRING; TZ
Summary: Encodes a thiamine biosynthetic gene that has a dual function in thiamine biosynthesis and mitochondrial DNA damage tolerance. It appears to be involved in producing the thiazole portion of thiamine (vitamin B1). A crystal structure of the protein reveals that it forms a 2-ring homo-octamer.
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Genomic context

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See THI1 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 3

Sequence:: Chromosome: 5; NC_003076.8 (22246519..22248332)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
Title: Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s.
Title: Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s.
Consistent with their contrasting regulatory role over stomatal closure, THI1 suppressed CPK33 kinase activity in vitro.
Title: THI1, a Thiamine Thiazole Synthase, Interacts with Ca2+-Dependent Protein Kinase CPK33 and Modulates the S-Type Anion Channels and Stomatal Closure in Arabidopsis.
The THI1 alanine to valine substitution directly affects the rate of the thiazole biosynthesis.
Title: THI1, a protein involved in the biosynthesis of thiamin in Arabidopsis thaliana: structural analysis of THI1(A140V) mutant.
Data indicate that the THI4 (AT5G54770) and the ThiM (AT3G24030)/THI4 mutants did not grow, as was expected, and the ThiM single mutant grew normally.
Title: Identification of the thiamin salvage enzyme thiazole kinase in Arabidopsis and maize.
THI1 is targeted to both chloroplasts and mitochondria and is expressed in both shoots and roots during vegetative growth but more active in shoot tissues of mature plants. Expression is affected by high salinity, sugar deprivation and flooding.
Title: Functional characterization of the thi1 promoter region from Arabidopsis thaliana.
A point mutation in the thi1 gene, involved in the synthesis of thiamin is responsible for the tz phenotype where the cotyledon is green but the leaves are white, and the plants die early during development.
Title: Point mutation is responsible for Arabidopsis tz-201 mutant phenotype affecting thiamin biosynthesis.
analysis of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana
Title: Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
Localization to endosomes.
Title: Functional differentiation of endosomes in Arabidopsis cells.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_124858.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	HDA more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within oxazole or thiazole biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within oxazole or thiazole biosynthetic process	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within response to cold	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within thiamine biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within thiamine biosynthetic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast envelope	HDA more info	PubMed
located_in chloroplast stroma	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in plastid	HDA more info	PubMed
located_in stromule	IDA Inferred from Direct Assay more info	PubMed
located_in thylakoid	HDA more info	PubMed

General protein information

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Preferred Names: thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)

NP_200288.1

THI1; FUNCTIONS IN: protein homodimerization activity, zinc ion binding; INVOLVED IN: oxazole or thiazole biosynthetic process, response to cold, thiamin biosynthetic process, response to DNA damage stimulus; LOCATED IN: in 6 components; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine biosynthesis Thi4 protein (InterPro:IPR002922); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

22246519..22248332

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_124858.4 → NP_200288.1 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_200288.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q38814

UniProtKB/TrEMBL

A0A178UNL2, A0A5S9YEA7

Conserved Domains (1) summary

PLN02661
Location:2 → 349

PLN02661; Putative thiazole synthesis