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AOS allene oxide synthase [ Arabidopsis thaliana (thale cress) ]

Gene ID: 834273, updated on 18-Sep-2024

Summary

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Official Symbol
AOS
Official Full Name
allene oxide synthase
Primary source
TAIR:AT5G42650
Locus tag
AT5G42650
See related
Araport:AT5G42650
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
allene oxide synthase; CYP74A; CYTOCHROME P450 74A; DDE2; DELAYED DEHISCENCE 2
Summary
Encodes a member of the cytochrome p450 CYP74 gene family that functions as an allene oxide synthase. This enzyme catalyzes dehydration of the hydroperoxide to an unstable allene oxide in the JA biosynthetic pathway. It shows a dual catalytic activity, the major one being a 13-AOS but also expressing a 9-AOS activity.
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Genomic context

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See AOS in Genome Data Viewer
Location:
chromosome: 5
Exon count:
1
Sequence:
Chromosome: 5; NC_003076.8 (17097564..17099595, complement)

Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene C2H2 and C2HC zinc fingers superfamily protein Neighboring gene pseudo Neighboring gene Disease resistance-responsive (dirigent-like protein) family protein Neighboring gene DNA-directed RNA polymerase subunit beta (DUF616)

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Allene oxide synthase from Arabidopsis thaliana (CYP74A1) exhibits dual specificity that is regulated by monomer-micelle association. Hughes RK, et al. FEBS Lett, 2006 Jul 24. PMID 16831431
  2. Molecular cloning of an allene oxide synthase: a cytochrome P450 specialized for the metabolism of fatty acid hydroperoxides. Song WC, et al. Proc Natl Acad Sci U S A, 1993 Sep 15. PMID 8378325, Free PMC Article
  3. Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP 74), the first enzyme of the octadecanoid pathway to jasmonates. Laudert D, et al. Plant Mol Biol, 1996 May. PMID 8756596
  4. Allene oxide synthase: a major control point in Arabidopsis thaliana octadecanoid signalling. Laudert D, et al. Plant J, 1998 Sep. PMID 9778849
  5. Structure and regulation of the Arabidopsis thaliana allene oxide synthase gene. Kubigsteltig I, et al. Planta, 1999 Jun. PMID 10420644

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. AOS and HPL are plastid envelope enzymes in Arabidopsis thaliana but accumulate at different locations. Biochemical experiments identified AOS as a constituent of complexes also containing lipoxygenase 2 (LOX2) and allene oxide cyclase (AOC), which catalyze consecutive steps in JA precursor biosynthesis, while excluding the concurrent HPL reaction.
    Title: Substrate channeling in oxylipin biosynthesis through a protein complex in the plastid envelope of Arabidopsis thaliana.
  2. Knockout of the rhomboid-like proteases, AtRBL8 and AtRBL9, reduces expression of allene oxide synthase.
    Title: Rhomboid proteins in the chloroplast envelope affect the level of allene oxide synthase in Arabidopsis thaliana.
  3. Theoretical calculations successfully map the whole AOS pathway with 13(S)-hydroperoxy linolenic and linoleic acid as substrates.
    Title: The reaction mechanism of allene oxide synthase: Interplay of theoretical QM/MM calculations and experimental investigations.
  4. crystal structures of Arabidopsis thaliana AOS, free and in complex with substrate or intermediate analogues
    Title: Structural insights into the evolutionary paths of oxylipin biosynthetic enzymes.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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NM_123629.4

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables allene oxide synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables heme binding IEA
Inferred from Electronic Annotation
more info
  
enables hydro-lyase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables monooxygenase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen IEA
Inferred from Electronic Annotation
more info
  
enables oxygen binding ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within defense response TAS
Traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within defense response to fungus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within epoxygenase P450 pathway ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
acts_upstream_of_or_within jasmonic acid biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within jasmonic acid biosynthetic process TAS
Traceable Author Statement
more info
 PubMed 
involved_in oxylipin biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within oxylipin metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to fungus IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to jasmonic acid IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to wounding IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to wounding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in chloroplast HDA PubMed 
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in chloroplast ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
located_in chloroplast envelope HDA PubMed 
located_in chloroplast thylakoid HDA PubMed 
located_in chloroplast thylakoid membrane HDA PubMed 
located_in mitochondrion HDA PubMed 
located_in plastid HDA PubMed 
located_in plastoglobule HDA PubMed 
located_in thylakoid HDA PubMed 

General protein information

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Preferred Names
allene oxide synthase
NP_199079.1
  • allene oxide synthase (AOS); FUNCTIONS IN: hydro-lyase activity, allene oxide synthase activity, oxygen binding; INVOLVED IN: in 7 processes; LOCATED IN: in 7 components; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128); BEST Arabidopsis thaliana protein match is: hydroperoxide lyase 1 (TAIR:AT4G15440.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003076.8 Reference assembly

    Range
    17097564..17099595 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_123629.4 → NP_199079.1  allene oxide synthase [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_199079.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P93720, Q96242, Q9ZR51
    UniProtKB/TrEMBL
    A0A178UE59, A0A5S9YAV7
    Conserved Domains (1) summary
    PLN02648
    Location:39 → 518
    PLN02648; allene oxide synthase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96242.3

Gene LinkOut

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