HEN1 double-stranded RNA binding protein-related / DsRBD protein-like protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: HEN1
Official Full Name: double-stranded RNA binding protein-related / DsRBD protein-like protein
Primary source: TAIR:AT4G20910
Locus tag: AT4G20910
See related: Araport:AT4G20910
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: CORYMBOSA 2; CRM2; HUA ENHANCER 1; T13K14.70; T13K14_70
Summary: Encodes an enhancer of hua1 and hua2 tha acts to specify reproductive organ identities and to repress A gene function. HEN1 also shares AG's non-homeotic function in controlling floral determinacy. Mutants display corymb-like inflorescences. HEN1 is a methyltransferase that methylates miRNAs and siRNAs on the ribose of the last nucleotide. The 3'-end methylation probably protects the 3' ends of the small RNAs from uridylation.
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Genomic context

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See HEN1 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 10

Sequence:: Chromosome: 4; NC_003075.7 (11185936..11191123, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Investigating the Viral Suppressor HC-Pro Inhibiting Small RNA Methylation through Functional Comparison of HEN1 in Angiosperm and Bryophyte.
Title: Investigating the Viral Suppressor HC-Pro Inhibiting Small RNA Methylation through Functional Comparison of HEN1 in Angiosperm and Bryophyte.
De Novo Shoot Regeneration Controlled by HEN1 and TCP3/4 in Arabidopsis.
Title: De Novo Shoot Regeneration Controlled by HEN1 and TCP3/4 in Arabidopsis.
we show HESO1 may also act redundantly with URT1 in miRNA uridylation when HEN1 is fully competent
Title: Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis.
Light-activated HEN1 expression depended on the photoreceptors phytochrome A (phyA), phyB, cryptochrome 1 (cry1), and cry2 and key transcriptional regulators ELONGATED HYPOCOTYL5 (HY5) and HY5-HOMOLOG.
Title: HUA ENHANCER1 is involved in posttranscriptional regulation of positive and negative regulators in Arabidopsis photomorphogenesis.
Data suggest a mechanism of plant miRNA maturation which involves binding of the HEN1 methyltransferase to the DICER-LIKE 1 ribonuclease DCL1*RNA-binding protein HYL1*miRNA complex.
Title: Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins.
Data indicate that the ago1 mutation in hen1 suppressed micriRNA 3' modifications.
Title: Plant microRNAs display differential 3' truncation and tailing modifications that are ARGONAUTE1 dependent and conserved across species.
AGO1, HYL1, and HEN1 function in proximal-distal and vascular patterning of leaves.
Title: The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis.
AGO2 and HEN1 participate in the DCL2-mediated antiviral defense to ensure the survival of Turnip crinkle virus-infected plants at high temperature.
Title: Temperature-dependent survival of Turnip crinkle virus-infected arabidopsis plants relies on an RNA silencing-based defense that requires dcl2, AGO2, and HEN1.
Motif mapping analysis revealed that the amino acids located between positions 139 and 320 of Zucchini yellow mosaic virus HC-Pro were associated with Arabidopsis HEN1 interaction.
Title: The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro.
A mutation in RNA-dependent RNA polymerase 2 rescued the defects in miRNA methylation of hen1-2, revealing a previously unsuspected, negative influence of siRNAs on HEN1-mediated miRNA methylation.
Title: siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001203853.2

Supported by ESTs:

NM_118209.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables RNA methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables peptidyl-prolyl cis-trans isomerase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA methylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within RNAi-mediated antiviral immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within leaf proximal/distal pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within leaf vascular tissue pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within miRNA processing	NAS Non-traceable Author Statement more info
acts_upstream_of_or_within miRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of flower development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within siRNA processing	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within specification of floral organ identity	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: double-stranded RNA binding protein-related / DsRBD protein-like protein

NP_001190782.1

HUA ENHANCER 1 (HEN1); FUNCTIONS IN: RNA methyltransferase activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus, cytoplasm; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA-binding protein Lupus La (InterPro:IPR006630), Methyltransferase type 12 (InterPro:IPR013217); BEST Arabidopsis thaliana protein match is: double-stranded RNA-binding domain (DsRBD)-containing protein (TAIR:AT4G20920.1).

NP_567616.1

HUA ENHANCER 1 (HEN1); FUNCTIONS IN: RNA methyltransferase activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA-binding protein Lupus La (InterPro:IPR006630), Methyltransferase type 12 (InterPro:IPR013217); BEST Arabidopsis thaliana protein match is: double-stranded RNA-binding domain (DsRBD)-containing protein (TAIR:AT4G20920.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

11185936..11191123 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001203853.2 → NP_001190782.1 double-stranded RNA binding protein-related / DsRBD protein-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001190782.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O65309, Q945R3, Q9C5Q8, Q9SUC2

UniProtKB/TrEMBL

A0A178UWW8

Conserved Domains (1) summary

pfam12847
Location:715 → 827

Methyltransf_18; Methyltransferase domain
NM_118209.3 → NP_567616.1 double-stranded RNA binding protein-related / DsRBD protein-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_567616.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O65309, Q945R3, Q9C5Q8, Q9SUC2

UniProtKB/TrEMBL

A0A178UWW8

Conserved Domains (1) summary

pfam12847
Location:715 → 827

Methyltransf_18; Methyltransferase domain