AK-HSDH II aspartate kinase-homoserine dehydrogenase ii [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: AK-HSDH II
Official Full Name: aspartate kinase-homoserine dehydrogenase ii
Primary source: TAIR:AT4G19710
Locus tag: AT4G19710
See related: Araport:AT4G19710
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AK-HSDH; ASPARTATE KINASE-HOMOSERINE DEHYDROGENASE; aspartate kinase-homoserine dehydrogenase ii; T16H5.70; T16H5_70
Summary: Encodes a bifunctional aspartate kinase/homoserine dehydrogenase. These two activities catalyze the first and the third steps toward the synthesis of the essential amino acids threonine, isoleucine and methionine.
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Genomic context

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See AK-HSDH II in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 18

Sequence:: Chromosome: 4; NC_003075.7 (10724814..10729768)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that mutations in the Asp kinases (AKs) and dual-functional Asp kinase-homoserine dehydrogenases (AK-HSDHs) result in reduced transcript levels and gene product activitiee and increases in the contents of Asp-derived amino acids.
Title: Analysis of Loss-of-Function Mutants in Aspartate Kinase and Homoserine Dehydrogenase Genes Points to Complexity in the Regulation of Aspartate-Derived Amino Acid Contents.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_118091.3

Supported by ESTs:

NM_202847.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables NAD+ binding	ISS Inferred from Sequence or Structural Similarity more info
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables aspartate kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables homoserine dehydrogenase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within aspartate family amino acid biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in homoserine biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lysine biosynthetic process via diaminopimelate	IEA Inferred from Electronic Annotation more info
involved_in lysine biosynthetic process via diaminopimelate	ISS Inferred from Sequence or Structural Similarity more info
involved_in methionine biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in threonine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in threonine biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast stroma	HDA more info	PubMed

General protein information

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Preferred Names: aspartate kinase-homoserine dehydrogenase ii

NP_193706.2

aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II); FUNCTIONS IN: homoserine dehydrogenase activity, aspartate kinase activity; INVOLVED IN: aspartate family amino acid biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Homoserine dehydrogenase, catalytic (InterPro:IPR001342), Amino acid-binding ACT (InterPro:IPR002912), Aspartate/homoserine dehydrogenase, NAD-binding (InterPro:IPR005106), Aspartate kinase, conserved site (InterPro:IPR018042), Bifunctional aspartokinase/homoserine dehydrogenase I (InterPro:IPR011147), Aspartate kinase domain (InterPro:IPR001341), NAD(P)-binding domain (InterPro:IPR016040), Homoserine dehydrogenase, conserved site (InterPro:IPR019811); BEST Arabidopsis thaliana protein match is: aspartate kinase-homoserine dehydrogenase i (TAIR:AT1G31230.1); Has 17435 Blast hits to 17108 proteins in 2575 species: Archae - 421; Bacteria - 11654; Metazoa - 7; Fungi - 299; Plants - 259; Viruses - 0; Other Eukaryotes - 4795 (source: NCBI BLink).

NP_974576.1

aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II); FUNCTIONS IN: homoserine dehydrogenase activity, aspartate kinase activity; INVOLVED IN: aspartate family amino acid biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Homoserine dehydrogenase, catalytic (InterPro:IPR001342), Amino acid-binding ACT (InterPro:IPR002912), Aspartate/homoserine dehydrogenase, NAD-binding (InterPro:IPR005106), Aspartate kinase, conserved site (InterPro:IPR018042), NAD(P)-binding domain (InterPro:IPR016040), Aspartate kinase domain (InterPro:IPR001341), Bifunctional aspartokinase/homoserine dehydrogenase I (InterPro:IPR011147), Homoserine dehydrogenase, conserved site (InterPro:IPR019811); BEST Arabidopsis thaliana protein match is: aspartate kinase-homoserine dehydrogenase i (TAIR:AT1G31230.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

10724814..10729768

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_202847.3 → NP_974576.1 aspartate kinase-homoserine dehydrogenase ii [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_974576.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O81852, Q3E9Y8

UniProtKB/TrEMBL

A0A178V4Q7, A0A654FQY0, Q0WRP9

Conserved Domains (1) summary

PRK09436
Location:89 → 914

thrA; bifunctional aspartokinase I/homoserine dehydrogenase I; Provisional
NM_118091.3 → NP_193706.2 aspartate kinase-homoserine dehydrogenase ii [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_193706.2

Status: REVIEWED

UniProtKB/TrEMBL

A0A178V4Q7

Conserved Domains (6) summary

cd04921
Location:407 → 486

ACT_AKi-HSDH-ThrA-like_1; ACT domains of the bifunctional enzyme aspartokinase (AK) - homoserine dehydrogenase (HSDH)

cd04922
Location:488 → 553

ACT_AKi-HSDH-ThrA_2; ACT domains of the bifunctional enzyme aspartokinase (AK) - homoserine dehydrogenase (HSDH)

PRK09436
Location:89 → 836

thrA; bifunctional aspartokinase I/homoserine dehydrogenase I; Provisional

cd04257
Location:89 → 383

AAK_AK-HSDH; AAK_AK-HSDH: Amino Acid Kinase Superfamily (AAK), AK-HSDH; this CD includes the N-terminal catalytic domain of aspartokinase (AK) of the bifunctional enzyme AK - homoserine dehydrogenase (HSDH). These aspartokinases are found in bacteria (E. coli ...

pfam00742
Location:716 → 827

Homoserine_dh; Homoserine dehydrogenase

pfam03447
Location:564 → 700

NAD_binding_3; Homoserine dehydrogenase, NAD binding domain