Cysteine proteinases superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Full Name: Cysteine proteinases superfamily protein
Primary source: TAIR:AT4G01610
Locus tag: AT4G01610
See related: Araport:AT4G01610
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: T15B16.17; T15B16_17
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Genomic context

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See in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 11

Sequence:: Chromosome: 4; NC_003075.7 (694582..697206)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Endoplasmic reticulum-stress-induced programmed cell death is regulated positively by cathepsin B and negatively by PBA1, revealing a complex picture behind caspase-3-like activity in plants. Cathepsin B may execute its function after tonoplast rupture and works in parallel with VPE.[cathepsin B3]
Title: Two proteases with caspase-3-like activity, cathepsin B and proteasome, antagonistically control ER-stress-induced programmed cell death in Arabidopsis.
AtCathB2 and AtCathB3 are two closely related PLCPs which display subtle differences in their hydrolytic properties and substrate specificities. Both enzymes can act as endopeptidases and carboxydipeptidases.[CathB3]
Title: The two cathepsin B-like proteases of Arabidopsis thaliana are closely related enzymes with discrete endopeptidase and carboxydipeptidase activities.
Recombinant and native AtCathB3 had enzymatic activity against the synthetic caspase-3 substrate DEVD (Asp-Glu-Val-Asp) and were inhibited by synthetic caspase-3 inhibitors. cathepsin B does regulate PCD in both animal and plant cells suggesting that this protease may be part of an ancestral PCD pathway pre-existing the plant/animal divergence. [Cathepsin B3]
Title: Inhibition of cathepsin B by caspase-3 inhibitors blocks programmed cell death in Arabidopsis.
GBF1 is a transcriptional repressor of the AtCathB3 gene and affects germination kinetics of A. thaliana seeds. As AtCathB3 is also expressed during post-germination in cotyledons, a role for the AtCathB3-like protease in reserve mobilization is inferred.
Title: The AtCathB3 gene, encoding a cathepsin B-like protease, is expressed during germination of Arabidopsis thaliana and transcriptionally repressed by the basic leucine zipper protein GBF1.

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General gene information

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Homology

Orthologs from OrthoDB

NM_116392.4

Supported by ESTs:

NM_178950.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables cysteine-type endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within proteolysis	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular region	ISM Inferred from Sequence Model more info
located_in plant-type vacuole	HDA more info	PubMed
located_in secretory vesicle	HDA more info	PubMed

General protein information

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Preferred Names: Cysteine proteinases superfamily protein

NP_567215.1

Cysteine proteinases superfamily protein; FUNCTIONS IN: cysteine-type endopeptidase activity, cysteine-type peptidase activity; INVOLVED IN: proteolysis, regulation of catalytic activity; LOCATED IN: vacuole; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase C1A, papain (InterPro:IPR013128), Peptidase C1A, cathepsin B (InterPro:IPR015643), Peptidase C1A, papain C-terminal (InterPro:IPR000668), Peptidase, cysteine peptidase active site (InterPro:IPR000169), Peptidase C1A, propeptide (InterPro:IPR012599); BEST Arabidopsis thaliana protein match is: Cysteine proteinases superfamily protein (TAIR:AT1G02305.1); Has 7486 Blast hits to 7443 proteins in 692 species: Archae - 63; Bacteria - 158; Metazoa - 3254; Fungi - 4; Plants - 1745; Viruses - 146; Other Eukaryotes - 2116 (source: NCBI BLink).

NP_849281.1

Cysteine proteinases superfamily protein; FUNCTIONS IN: cysteine-type endopeptidase activity, cysteine-type peptidase activity; INVOLVED IN: proteolysis, regulation of catalytic activity; LOCATED IN: vacuole; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase C1A, papain (InterPro:IPR013128), Peptidase C1A, cathepsin B (InterPro:IPR015643), Peptidase C1A, papain C-terminal (InterPro:IPR000668), Peptidase, cysteine peptidase active site (InterPro:IPR000169), Peptidase C1A, propeptide (InterPro:IPR012599); BEST Arabidopsis thaliana protein match is: Cysteine proteinases superfamily protein (TAIR:AT1G02305.1); Has 7406 Blast hits to 7364 proteins in 687 species: Archae - 57; Bacteria - 153; Metazoa - 3244; Fungi - 4; Plants - 1710; Viruses - 146; Other Eukaryotes - 2092 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

694582..697206

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_116392.4 → NP_567215.1 Cysteine proteinases superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_567215.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94K85, Q9ZSI0

UniProtKB/TrEMBL

A0A178UYV4

Conserved Domains (2) summary

cd02620
Location:104 → 337

Peptidase_C1A_CathepsinB; Cathepsin B group; composed of cathepsin B and similar proteins, including tubulointerstitial nephritis antigen (TIN-Ag). Cathepsin B is a lysosomal papain-like cysteine peptidase which is expressed in all tissues and functions primarily as an ...

pfam08127
Location:43 → 85

Propeptide_C1; Peptidase family C1 propeptide
NM_178950.2 → NP_849281.1 Cysteine proteinases superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_849281.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A5S9XPB1, A0A654FL17

Conserved Domains (2) summary

cd02620
Location:104 → 337

Peptidase_C1A_CathepsinB; Cathepsin B group; composed of cathepsin B and similar proteins, including tubulointerstitial nephritis antigen (TIN-Ag). Cathepsin B is a lysosomal papain-like cysteine peptidase which is expressed in all tissues and functions primarily as an ...

pfam08127
Location:43 → 85

Propeptide_C1; Peptidase family C1 propeptide