TPC1 two-pore channel 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: TPC1
Official Full Name: two-pore channel 1
Primary source: TAIR:AT4G03560
Locus tag: AT4G03560
See related: Araport:AT4G03560
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATCCH1; ATTPC1; CALCIUM CHANNEL 1; FATTY ACID OXYGENATION UPREGULATED 2; FOU2; T5L23.5; two-pore channel 1; two-pore channel 1
Summary: Encodes a depolarization-activated Ca(2+) channel. Anti-sense experiments with this gene as well as Sucrose-H(+) symporters and complementation of yeast sucrose uptake mutant cch1 suggest that this protein mediates a voltage-activated Ca(2+)influx. Mutants lack detectable SV channel activity suggesting TPC1 is essential component of the SV channel. Patch clamp analysis of loss of function mutation indicates TPC1 does not affect Ca2+ signaling in response to abiotic and biotic stress.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See TPC1 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 23

Sequence:: Chromosome: 4; NC_003075.7 (1579929..1585980)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TPC1 vacuole SV channel gains further shape - voltage priming of calcium-dependent gating.
Title: TPC1 vacuole SV channel gains further shape - voltage priming of calcium-dependent gating.
Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway.
Title: Computational Analyses of the AtTPC1 (Arabidopsis Two-Pore Channel 1) Permeation Pathway.
Study results in mesophyll vacuoles from Arabidopsis show that the vacuolar K+-conducting TPC1 and TPK1/TPK3 channels act in concert to provide for Ca2+- and voltage-induced electrical excitability to the central organelle of plant cells.
Title: Voltage-dependent gating of SV channel TPC1 confers vacuole excitability.
Structural basis for activation of voltage sensor domains in an ion channel TPC1.
Title: Structural basis for activation of voltage sensor domains in an ion channel TPC1.
variant (TPC1D454N) depolarizes endomembranes by increasing cation fluxes towards the cytosol
Title: Control of basal jasmonate signalling and defence through modulation of intracellular cation flux capacity.
The ion selectivity of AtTPC1 was determined and compared with the selectivity of human TPC2. TPC1 is selective for Ca(2+) over Na(+), but nonselective among monovalent Li(+), Na(+), and K(+)). AtTPC1 could be converted to a Na(+)-selective channel by mimicking the selectivity filter of HsTPC2. Key residues were identified in the TPC filters that differentiate this selectivity. A Na+-selective mutant was characterized.
Title: Tuning the ion selectivity of two-pore channels.
TPC1 role in the systemic response to salt stress
Title: A ROS-Assisted Calcium Wave Dependent on the AtRBOHD NADPH Oxidase and TPC1 Cation Channel Propagates the Systemic Response to Salt Stress.
Structure-function analysis of the Arabidopsis TPC1 channel in planta confirmed that helix S10 operates as the major voltage-sensing site, with Glu450 and Glu478 identified as possible ion-pair partners for voltage-sensing Arg537.
Title: Gating of the two-pore cation channel AtTPC1 in the plant vacuole is based on a single voltage-sensing domain.
The domain architecture of TPC1 has been discussed.
Title: Two-pore Channels Enter the Atomic Era: Structure of Plant TPC Revealed.
This study discovered that the dimerization of a predicted helix within the carboxyl-terminus is essential for the activity of TPC1.
Title: The function of the two-pore channel TPC1 depends on dimerization of its carboxy-terminal helix.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

NM_116594.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables calcium channel activity	IGI Inferred from Genetic Interaction more info	PubMed
enables calcium channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calcium channel activity	ISS Inferred from Sequence or Structural Similarity more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables voltage-gated calcium channel activity	IGI Inferred from Genetic Interaction more info	PubMed
enables voltage-gated calcium channel activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within calcium ion transport	IC Inferred by Curator more info	PubMed
acts_upstream_of_or_within calcium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of jasmonic acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of stomatal movement	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within seed germination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	HDA more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed
located_in plant-type vacuole	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in vacuolar membrane	IDA Inferred from Direct Assay more info	PubMed
located_in vacuole	HDA more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: two-pore channel 1

NP_567258.1

two-pore channel 1 (TPC1); FUNCTIONS IN: voltage-gated calcium channel activity, calcium channel activity; INVOLVED IN: regulation of jasmonic acid biosynthetic process, calcium ion transport, calcium-mediated signaling, seed germination, regulation of stomatal movement; LOCATED IN: vacuolar membrane, plasma membrane, vacuole, plant-type vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: EF-HAND 2 (InterPro:IPR018249), Ion transport (InterPro:IPR005821), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

1579929..1585980

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_116594.5 → NP_567258.1 two-pore channel 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_567258.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q948T1, Q94KI8, Q9ZT83

UniProtKB/TrEMBL

A0A5S9XPM9, A0A654FLY1, B9DFD5

Conserved Domains (2) summary

cd00051
Location:326 → 391

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam00520
Location:429 → 675

Ion_trans; Ion transport protein