U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SIP2 seed imbibition 2 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 824919, updated on 18-Sep-2024

Summary

Go to the top of the page Help
Official Symbol
SIP2
Official Full Name
seed imbibition 2
Primary source
TAIR:AT3G57520
Locus tag
AT3G57520
See related
Araport:AT3G57520
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
AtSIP2; raffinose synthase 2; RS2; seed imbibition 2
Summary
SIP2 encodes a raffinose-specific alpha-galactosidase that catalyzes the breakdown of raffinose into alpha-galatose and sucrose. This enzyme may function in unloading raffinose from the phloem as part of sink metabolism. Although it was originally predicted to act as a raffinose synthase (RS), that activity was not observed for recombinant SIP2.
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See SIP2 in Genome Data Viewer
Location:
chromosome: 3
Exon count:
14
Sequence:
Chromosome: 3; NC_003074.8 (21288742..21293171, complement)

Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene fission regulator-like protein Neighboring gene Pectin lyase-like superfamily protein Neighboring gene calcium-dependent protein kinase 32 Neighboring gene Remorin family protein

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern. Peters S, et al. Plant Cell Physiol, 2010 Oct. PMID 20739305
  2. Conservation and molecular dissection of ROUGH SHEATH2 and ASYMMETRIC LEAVES1 function in leaf development. Theodoris G, et al. Proc Natl Acad Sci U S A, 2003 May 27. PMID 12750468, Free PMC Article
  3. Galactinol and raffinose constitute a novel function to protect plants from oxidative damage. Nishizawa A, et al. Plant Physiol, 2008 Jul. PMID 18502973, Free PMC Article
  4. Arabidopsis plasmodesmal proteome. Fernandez-Calvino L, et al. PLoS One, 2011 Apr 20. PMID 21533090, Free PMC Article
  5. Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana. Salanoubat M, et al. Nature, 2000 Dec 14. PMID 11130713

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. ATSIP2 encodes a Raf-specific alkaline alpha-galactosidase (and not a raffinose synthase) and suggest that it is involved in sink metabolism, most probably in phloem unloading of Raf.
    Title: Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern.
Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help
NM_115612.3
NM_180384.1
NM_202722.1

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
NOT enables galactinol-sucrose galactosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables galactinol-sucrose galactosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables raffinose alpha-galactosidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within raffinose catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to oxidative stress IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm ISM
Inferred from Sequence Model
more info
  
located_in plasmodesma HDA PubMed 

General protein information

Go to the top of the page Help
Preferred Names
seed imbibition 2
NP_191311.1
  • seed imbibition 2 (SIP2); CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 1 (TAIR:AT1G55740.1); Has 528 Blast hits to 504 proteins in 147 species: Archae - 29; Bacteria - 95; Metazoa - 0; Fungi - 82; Plants - 312; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).
NP_850715.1
  • seed imbibition 2 (SIP2); CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 1 (TAIR:AT1G55740.1); Has 515 Blast hits to 494 proteins in 148 species: Archae - 29; Bacteria - 95; Metazoa - 0; Fungi - 82; Plants - 301; Viruses - 0; Other Eukaryotes - 8 (source: NCBI BLink).
NP_974451.1
  • seed imbibition 2 (SIP2); CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 1 (TAIR:AT1G55740.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003074.8 Reference assembly

    Range
    21288742..21293171 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_115612.3 → NP_191311.1  seed imbibition 2 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_191311.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q0WWI0, Q3EAI2, Q3EAI3, Q94A08, Q9SCM1
    UniProtKB/TrEMBL
    A0A178VJQ6
    Conserved Domains (1) summary
    PLN02219
    Location:1 → 773
    PLN02219; probable galactinol--sucrose galactosyltransferase 2

  2. NM_202722.1 → NP_974451.1  seed imbibition 2 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_974451.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    F4J3E6
    Conserved Domains (1) summary
    cl07893
    Location:1 → 551
    AmyAc_family; Alpha amylase catalytic domain family

  3. NM_180384.1 → NP_850715.1  seed imbibition 2 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_850715.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VJQ6, A0A5S9XMF3, A0A654FIP0
    Conserved Domains (1) summary
    cl07893
    Location:1 → 656
    AmyAc_family; Alpha amylase catalytic domain family
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q94A08.2

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.