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CYP94B3 cytochrome P450, family 94, subfamily B, polypeptide 3 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 824011, updated on 18-Sep-2024

Summary

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Official Symbol
CYP94B3
Official Full Name
cytochrome P450, family 94, subfamily B, polypeptide 3
Primary source
TAIR:AT3G48520
Locus tag
AT3G48520
See related
Araport:AT3G48520
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
cytochrome P450; family 94; polypeptide 3; subfamily B
Summary
CYP94B3 is a jasmonoyl-isoleucine-12-hydroxylase that catalyzes the formation of 12-OH-JA-Ile from JA-Ile. By reducing the levels of this the biologically active phytohormone, CYP94B3 attenuates the jasmonic acid signaling cascade. CYP94B3 transcript levels rise in response to wounding.
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Genomic context

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See CYP94B3 in Genome Data Viewer
Location:
chromosome: 3
Exon count:
1
Sequence:
Chromosome: 3; NC_003074.8 (17974853..17976774, complement)

Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene AtIII18x5-like protein Neighboring gene tRNA-Tyr Neighboring gene ncRNA Neighboring gene pseudo

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite. Kitaoka N, et al. Phytochemistry, 2014 Mar. PMID 24467969
  2. Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate. Kitaoka N, et al. Plant Cell Physiol, 2011 Oct. PMID 21849397
  3. WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis. Krishnamurthy P, et al. Physiol Plant, 2021 Jul. PMID 33619745
  4. Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates. Widemann E, et al. Phytochemistry, 2015 Sep. PMID 26164240
  5. CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection. Aubert Y, et al. J Exp Bot, 2015 Jul. PMID 25903915, Free PMC Article

See all (11) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis.
    Title: WRKY9 transcription factor regulates cytochrome P450 genes CYP94B3 and CYP86B1, leading to increased root suberin and salt tolerance in Arabidopsis.
  2. we created the double and triple knock-out mutants of three CYP94 genes, CYP94B1, CYP94B3, and CYP94C1. The mutations blocked the oxidation steps and caused JA-Ile to accumulate 3-4-fold the WT levels in the wounded leaves.
    Title: Mutations in jasmonoyl-L-isoleucine-12-hydroxylases suppress multiple JA-dependent wound responses in Arabidopsis thaliana.
  3. CYP94B3 and CYP94C1 are responsible for the production of oxidized derivatives of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine in wounded plants.
    Title: Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates.
  4. These results highlight the dual function of CYP94B3/C1 in antimicrobial defence.
    Title: CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection.
  5. It was found that CYP94B3 also catalyzes the hydroxylation of jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and that these hydroxyl compounds accumulated after wounding and possessed lower activity than non-hydroxylated compounds.(cytohrome P-450 94B3)
    Title: CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite.
  6. These results identify CYP94B3/C1-mediated oxidation as a major catabolic route for turning over the JA-Ile hormone.
    Title: Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover.
  7. CYP94B3 plays a major regulatory role in controlling the level of jasmonyl-L-isoleucine in plants.
    Title: Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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NM_114710.3

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables heme binding IEA
Inferred from Electronic Annotation
more info
  
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables jasmonoyl-isoleucine-12-hydroxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within anther development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within defense response to insect IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within fruit development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within jasmonic acid metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within pollen development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to wounding IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within stigma development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in endoplasmic reticulum membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular region ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
cytochrome P450, family 94, subfamily B, polypeptide 3
NP_190421.1
  • ''cytochrome P450, family 94, subfamily B, polypeptide 3'' (CYP94B3); FUNCTIONS IN: electron carrier activity, monooxygenase activity, iron ion binding, oxygen binding, heme binding; INVOLVED IN: oxidation reduction; LOCATED IN: endomembrane system; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 94, subfamily B, polypeptide 1 (TAIR:AT5G63450.1); Has 27655 Blast hits to 27536 proteins in 1435 species: Archae - 44; Bacteria - 2616; Metazoa - 10459; Fungi - 5693; Plants - 7745; Viruses - 3; Other Eukaryotes - 1095 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003074.8 Reference assembly

    Range
    17974853..17976774 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_114710.3 → NP_190421.1  cytochrome P450, family 94, subfamily B, polypeptide 3 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_190421.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q9SMP5
    UniProtKB/TrEMBL
    A0A178V8H3, A0A5S9XJ92
    Conserved Domains (1) summary
    cl12078
    Location:46 → 501
    p450; Cytochrome P450
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9SMP5.1

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