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NIT2 nitrilase 2 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 823555, updated on 18-Sep-2024

Summary

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Official Symbol
NIT2
Official Full Name
nitrilase 2
Primary source
TAIR:AT3G44300
Locus tag
AT3G44300
See related
Araport:AT3G44300
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
AtNIT2; nitrilase 2; NITRILASE 2; NITRILE AMINOHYDROLASE
Summary
Encodes an enzyme that catalyzes the hydrolysis of indole-3-acetonitrile (IAN) to indole-3-acetic acid (IAA) (nitrile aminohydrolase, EC 3.5.5.1) and IAN to indole-3-acetamide (IAM) at lower levels. Mutants have reduced sensitivity to IAN and are sensitive to IAA. This enzyme likely participates in other non-auxin-related metabolic pathways.
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Genomic context

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See NIT2 in Genome Data Viewer
Location:
chromosome: 3
Exon count:
5
Sequence:
Chromosome: 3; NC_003074.8 (15983199..15985535)

Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene NAC domain containing protein 60 Neighboring gene ncRNA Neighboring gene nitrilase 1 Neighboring gene nitrilase 3

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. ATAF2, a NAC transcription factor, binds to the promoter and regulates NIT2 gene expression involved in auxin biosynthesis. Huh SU, et al. Mol Cells, 2012 Sep. PMID 22965747, Free PMC Article
  2. Indole-3-acetic acid is synthesized from L-tryptophan in roots of Arabidopsis thaliana. Müller A, et al. Planta, 1998 Oct. PMID 9763705
  3. Occurrence and formation of indole-3-acetamide in Arabidopsis thaliana. Pollmann S, et al. Planta, 2002 Nov. PMID 12430025
  4. Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster. Vorwerk S, et al. Planta, 2001 Mar. PMID 11525507
  5. Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana. Bartel B, et al. Proc Natl Acad Sci U S A, 1994 Jul 5. PMID 8022831, Free PMC Article

See all (10) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. ATAF2 regulates NIT2 gene expression via NIT2 promoter binding.
    Title: ATAF2, a NAC transcription factor, binds to the promoter and regulates NIT2 gene expression involved in auxin biosynthesis.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

NM_114298.3

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables indole-3-acetonitrile nitrilase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables indole-3-acetonitrile nitrile hydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nitrilase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within indoleacetic acid biosynthetic process TAS
Traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to bacterium IEP
Inferred from Expression Pattern
more info
  
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
located_in plasma membrane ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
nitrilase 2
NP_190016.1
  • nitrilase 2 (NIT2); FUNCTIONS IN: indole-3-acetonitrile nitrilase activity, nitrilase activity, indole-3-acetonitrile nitrile hydratase activity; INVOLVED IN: response to cadmium ion, indoleacetic acid biosynthetic process, response to bacterium; LOCATED IN: cellular_component unknown; EXPRESSED IN: cotyledon, cultured cell; CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 1 (TAIR:AT3G44310.3); Has 7677 Blast hits to 7598 proteins in 1742 species: Archae - 157; Bacteria - 4958; Metazoa - 446; Fungi - 568; Plants - 325; Viruses - 11; Other Eukaryotes - 1212 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003074.8 Reference assembly

    Range
    15983199..15985535
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_114298.3 → NP_190016.1  nitrilase 2 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_190016.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P32962, Q96505
    UniProtKB/TrEMBL
    A0A178V952, A0A384LD03, O04907, Q1LYZ1
    Conserved Domains (1) summary
    PLN02504
    Location:1 → 339
    PLN02504; nitrilase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32962.1

Gene LinkOut

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