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PREP1 presequence protease 1 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 821451, updated on 18-Sep-2024

Summary

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Official Symbol
PREP1
Official Full Name
presequence protease 1
Primary source
TAIR:AT3G19170
Locus tag
AT3G19170
See related
Araport:AT3G19170
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
ATPREP1; ATZNMP; presequence protease 1
Summary
Zinc metalloprotease pitrilysin subfamily A. Signal peptide degrading enzyme targeted to mitochondria and chloroplasts. Expressed only in siliques and flowers
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Genomic context

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See PREP1 in Genome Data Viewer
Location:
chromosome: 3
Exon count:
20
Sequence:
Chromosome: 3; NC_003074.8 (6625377..6632164, complement)

Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene KIP-related protein 6 Neighboring gene ATP/ADP isopentenyltransferase Neighboring gene plastid division protein Neighboring gene AP2/B3-like transcriptional factor family protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Characterization of a novel zinc metalloprotease involved in degrading targeting peptides in mitochondria and chloroplasts. Moberg P, et al. Plant J, 2003 Dec. PMID 14617063
  2. Isolation and identification of a novel mitochondrial metalloprotease (PreP) that degrades targeting presequences in plants. Stahl A, et al. J Biol Chem, 2002 Nov 1. PMID 12138166
  3. Binding of divalent cations is essential for the activity of the organellar peptidasome in Arabidopsis thaliana, AtPreP. Bäckman HG, et al. FEBS Lett, 2009 Sep 3. PMID 19646442
  4. Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2. Bhushan S, et al. Plant Cell Physiol, 2005 Jun. PMID 15827031
  5. Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2. Glaser E, et al. Biol Chem, 2006 Oct-Nov. PMID 17081117

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. AtPrePI and AtPrePII are metalloproteases that degrade targeting peptides in mitochondria and chloroplasts. AtPrePI showed a preference for the N-terminal amphiphilic alpha-helix and positively charged amino acid residues.
    Title: Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2.
  2. The Arg/N-degron pathway targets transcription factors and regulates specific genes.
    Title: The Arg/N-degron pathway targets transcription factors and regulates specific genes.
  3. Data show that the AtPreP presequence has the capacity to target GFP into the mitochondrial matrix of all three species.
    Title: Targeting capacity and conservation of PreP homologues localization in mitochondria of different species.
  4. AtPreP functions as a peptidasome that degrades targeting peptides and other small unstructured peptides. In addition to Zn located in the catalytic site, AtPreP also contains two Mg-binding sites. [PreP1]
    Title: Binding of divalent cations is essential for the activity of the organellar peptidasome in Arabidopsis thaliana, AtPreP.
  5. The AtPreP peptidasome is required for efficient plant growth and organelle function particularly during early development.
    Title: Deletion of an organellar peptidasome PreP affects early development in Arabidopsis thaliana.
  6. AtPreP1 and AtPreP2 genes are differentially expressed in Arabidopsis
    Title: Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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NM_001203003.1
NM_112804.5

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metalloendopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within protein processing IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in apoplast HDA PubMed 
located_in chloroplast HDA PubMed 
located_in chloroplast IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chloroplast envelope HDA PubMed 
located_in chloroplast stroma HDA PubMed 
located_in cytosol HDA PubMed 
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
presequence protease 1
NP_001189932.1
  • presequence protease 1 (PREP1); CONTAINS InterPro DOMAIN/s: Peptidase M16, C-terminal (InterPro:IPR007863), Peptidase M16C associated (InterPro:IPR013578), Peptidase M16, N-terminal (InterPro:IPR011765), Metalloenzyme, LuxS/M16 peptidase-like, metal-binding (InterPro:IPR011249), Peptidase M16, core (InterPro:IPR011237); BEST Arabidopsis thaliana protein match is: presequence protease 2 (TAIR:AT1G49630.2).
NP_188548.2
  • presequence protease 1 (PREP1); FUNCTIONS IN: metalloendopeptidase activity; INVOLVED IN: response to cadmium ion, proteolysis, protein maturation by peptide bond cleavage; LOCATED IN: mitochondrion, apoplast, chloroplast stroma, chloroplast, chloroplast envelope; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase M16, C-terminal (InterPro:IPR007863), Peptidase M16C associated (InterPro:IPR013578), Peptidase M16, N-terminal (InterPro:IPR011765), Metalloenzyme, LuxS/M16 peptidase-like, metal-binding (InterPro:IPR011249), Peptidase M16, core (InterPro:IPR011237); BEST Arabidopsis thaliana protein match is: presequence protease 2 (TAIR:AT1G49630.2); Has 4222 Blast hits to 4196 proteins in 1321 species: Archae - 9; Bacteria - 2812; Metazoa - 224; Fungi - 268; Plants - 81; Viruses - 0; Other Eukaryotes - 828 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003074.8 Reference assembly

    Range
    6625377..6632164 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_112804.5 → NP_188548.2  presequence protease 1 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_188548.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q8RUN6, Q9LJL3
    UniProtKB/TrEMBL
    A0A178VHU1, A0A5S9XDK2, A0A654F8L9
    Conserved Domains (1) summary
    COG1026
    Location:106 → 1080
    Cym1; Zn-dependent peptidase, M16 (insulinase) family [Posttranslational modification, protein turnover, chaperones]

  2. NM_001203003.1 → NP_001189932.1  presequence protease 1 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_001189932.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A5S9XDK2, A0A654F8L9, F4JA10
    Conserved Domains (4) summary
    COG1026
    Location:106 → 1069
    Cym1; Zn-dependent peptidase, M16 (insulinase) family [Posttranslational modification, protein turnover, chaperones]
    pfam00675
    Location:154 → 248
    Peptidase_M16; Insulinase (Peptidase family M16)
    pfam05193
    Location:290 → 476
    Peptidase_M16_C; Peptidase M16 inactive domain
    pfam08367
    Location:557 → 801
    M16C_assoc; Peptidase M16C associated
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9LJL3.2

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