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ATSPO11-1 Spo11/DNA topoisomerase VI, subunit A protein [ Arabidopsis thaliana (thale cress) ]

Gene ID: 820506, updated on 18-Sep-2024

Summary

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Official Symbol
ATSPO11-1
Official Full Name
Spo11/DNA topoisomerase VI, subunit A protein
Primary source
TAIR:AT3G13170
Locus tag
AT3G13170
See related
Araport:AT3G13170
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
SPO11-1
Summary
Encodes AtSPO11-1, one of the three Arabidopsis homologues of the archaeal DNA topoisomerase VIA subunit (topo VIA). Required for meiotic recombination. AtSPO11-1 and AtSPO11-2 have overlapping functions (i.e. both required for meiotic recombination) whereas AtSPO11-3 functions in DNA replication. AtSPO11-1 accumulates in foci in early G2. At 1 h post-S phase, no foci are observed, but by 3 h a majority (80%) of meiocytes at this time point contain >50 foci. However, by 5 h, AtSPO11-1 foci are no longer detectable. This suggests that the protein undergoes a rapid cycle of accumulation and disappearance in meiocytes over a period of between 1 and 5 h post-S phase.
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Genomic context

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See ATSPO11-1 in Genome Data Viewer
Location:
chromosome: 3
Exon count:
15
Sequence:
Chromosome: 3; NC_003074.8 (4231395..4234206, complement)

Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene hydroxyproline-rich glycoprotein family protein Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene uncharacterized protein Neighboring gene NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana. Chen HW, et al. Nucleic Acids Res, 2024 May 8. PMID 38499490, Free PMC Article
  2. Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions. Choi K, et al. Genome Res, 2018 Apr. PMID 29530928, Free PMC Article
  3. A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis. Shingu Y, et al. FEBS J, 2010 May. PMID 20423461
  4. AtSPO11-1 is necessary for efficient meiotic recombination in plants. Grelon M, et al. EMBO J, 2001 Feb 1. PMID 11157765, Free PMC Article
  5. Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern. Sprink T, et al. Int J Mol Sci, 2021 Aug 28. PMID 34502253, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.
    Title: Biochemical characterization of the meiosis-essential yet evolutionarily divergent topoisomerase VIB-like protein MTOPVIB from Arabidopsis thaliana.
  2. Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.
    Title: Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.
  3. As transposon mobility in plant genomes is restricted by DNA methylation, we used the met1 DNA methyltransferase mutant to investigate the role of heterochromatin in SPO11-1-oligo distributions. Epigenetic activation of meiotic DSBs in proximity to centromeres and transposons occurred in met1 mutants, coincident with reduced nucleosome occupancy, gain of transcription, and H3K4me3
    Title: Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions.
  4. Results identified a group of spo11-1 mutants with defects in meiosis that specifically correlate with the defects in DNA binding at an identified DNA binding surface on SPO11-1.
    Title: A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis.
  5. Data show that the catalytically active tyrosine residues of SPO11-1 are required for meiotic double-strand break induction in Arabidopsis.
    Title: The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis.
  6. AtSPO11-1 and AtSPO11-2 genetically interact and function in meiotic recombination. [AtSPO11-1]
    Title: Arabidopsis SPO11-2 functions with SPO11-1 in meiotic recombination.
Submit: New GeneRIF Correction

General gene information

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NM_001338034.1
NM_001338035.1
NM_112156.2

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within chiasma assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within homologous chromosome pairing at meiosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within maintenance of meiotic sister chromatid cohesion IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within meiotic DNA double-strand break formation IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within meiotic DNA double-strand break processing IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within reciprocal meiotic recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
Spo11/DNA topoisomerase VI, subunit A protein
NP_187923.1
  • ATSPO11-1; CONTAINS InterPro DOMAIN/s: Spo11/DNA topoisomerase VI, subunit A, N-terminal (InterPro:IPR013049), Meiotic recombination, Spo11 (InterPro:IPR013048), Spo11/DNA topoisomerase VI, subunit A (InterPro:IPR002815); BEST Arabidopsis thaliana protein match is: Spo11/DNA topoisomerase VI, subunit A protein (TAIR:AT5G02820.1); Has 908 Blast hits to 908 proteins in 313 species: Archae - 216; Bacteria - 16; Metazoa - 170; Fungi - 122; Plants - 151; Viruses - 0; Other Eukaryotes - 233 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003074.8 Reference assembly

    Range
    4231395..4234206 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_112156.2 → NP_187923.1  Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_187923.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q9C5S3, Q9LK56, Q9M4A2
    UniProtKB/TrEMBL
    A0A178VGV5, A0A5S9XC69, A0A654FGW4
    Conserved Domains (1) summary
    COG1697
    Location:6 → 357
    Spo11; DNA topoisomerase VI, subunit A [Replication, recombination and repair]

  2. NM_001338034.1 → NP_001326966.1  Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1I9LQV8

  3. NM_001338035.1 → NP_001326967.1  Spo11/DNA topoisomerase VI, subunit A protein [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1I9LQV8
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9M4A2.1

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