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CAO chloroplast signal recognition particle component (CAO) [ Arabidopsis thaliana (thale cress) ]

Gene ID: 819358, updated on 18-Sep-2024

Summary

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Official Symbol
CAO
Official Full Name
chloroplast signal recognition particle component (CAO)
Primary source
TAIR:AT2G47450
Locus tag
AT2G47450
See related
Araport:AT2G47450
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
CHAOS; CHLOROPLAST SIGNAL RECOGNITION PARTICLE 43; CPSRP43; T30B22.25
Summary
A component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. It is downregulated in response to high light. It recognizes the DPLG motif in Lhcb1.
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Genomic context

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See CAO in Genome Data Viewer
Location:
chromosome: 2
Exon count:
1
Sequence:
Chromosome: 2; NC_003071.7 (19472573..19474387)

Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Signal transduction histidine kinase Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene myb domain protein 12 Neighboring gene thioredoxin family protein Neighboring gene DUF3511 domain protein, putative (DUF3511)

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone. Siegel A, et al. J Mol Biol, 2020 Dec 4. PMID 33188783, Free PMC Article
  2. A chromodomain protein encoded by the arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Klimyuk VI, et al. Plant Cell, 1999 Jan. PMID 9878634, Free PMC Article
  3. Involvement of the chloroplast signal recognition particle cpSRP43 in acclimation to conditions promoting photooxidative stress in Arabidopsis. Klenell M, et al. Plant Cell Physiol, 2005 Jan. PMID 15659446
  4. Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43. Stengel KF, et al. Science, 2008 Jul 11. PMID 18621669
  5. Mechanism of an ATP-independent protein disaggregase: I. structure of a membrane protein aggregate reveals a mechanism of recognition by its chaperone. Nguyen TX, et al. J Biol Chem, 2013 May 10. PMID 23525109, Free PMC Article

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Chloroplast SRP43 autonomously protects chlorophyll biosynthesis proteins against heat shock.
    Title: Chloroplast SRP43 autonomously protects chlorophyll biosynthesis proteins against heat shock.
  2. A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone.
    Title: A Disorder-to-Order Transition Activates an ATP-Independent Membrane Protein Chaperone.
  3. Site-directed mutagenesis identified a class of cpSRP43 mutants that bind tightly to the L18 sequence but are defective in chaperoning full-length chlorophyll a/b-binding proteins. These mutations mapped to hydrophobic surfaces near the bridging helix and the beta-hairpins lining the ankyrin repeat motifs of cpSRP43, suggesting that these regions are potential sites for interaction with the client transmembrane domains .
    Title: Two distinct sites of client protein interaction with the chaperone cpSRP43.
  4. CpSRP54 drives cpSRP43 to an active state, binding substrate in the H2O compartment. Bidentate interactions with Alb3 drive it to partial inactivity, selectively releasing LHCP transmembrane domains in a productive unloading complex at the membrane.
    Title: Conformational dynamics of a membrane protein chaperone enables spatially regulated substrate capture and release.
  5. cpSRP43 chromodomain 3 interacts with a linear motif in the Alb3 C-terminal tail. Study describes the structural basis for selectivity of CPSRP43 chromodomains 2 and 3 for their respective ligands cpSRP54 and Alb3.
    Title: Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction.
  6. results support the conclusion that substrate binding to the chloroplast SRP is modulated by protein structural dynamics in which a major role of cpSRP54 is to improve substrate binding efficiency to the cpSRP.
    Title: Regulation of Structural Dynamics within a Signal Recognition Particle Promotes Binding of Protein Targeting Substrates.
  7. The action of cpSRP43 can be dissected into two steps, initial recognition, during which cpSRP43 binds specifically to a recognition motif on the surface of the aggregate, followed by aggregate remodeling.
    Title: Mechanism of an ATP-independent protein disaggregase: II. distinct molecular interactions drive multiple steps during aggregate disassembly.
  8. A mechanism for recognition of the LHC aggregate by cpSRP43 elucidated by a biophysical and biochemical approach.
    Title: Mechanism of an ATP-independent protein disaggregase: I. structure of a membrane protein aggregate reveals a mechanism of recognition by its chaperone.
  9. cpSRP43 inserts into a dimeric Alb3 translocation pore during cpSRP-dependent delivery of light-harvesting chlorophyll a,b-binding proteins. [SRP43]
    Title: Interaction studies between the chloroplast signal recognition particle subunit cpSRP43 and the full-length translocase Alb3 reveal a membrane-embedded binding region in Alb3 protein.
  10. The authors report the crystal structure of the chloroplast signal recognition particle (cpSRP) core from Arabidopsis thaliana, with the cpSRP54 tail comprising an arginine-rich motif bound to the second chromodomain of cpSRP43.
    Title: Chromodomains read the arginine code of post-translational targeting.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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NM_130313.3

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within protein import into chloroplast thylakoid membrane TAS
Traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to high light intensity IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in chloroplast HDA PubMed 
located_in chloroplast IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in chloroplast envelope HDA PubMed 
located_in chloroplast thylakoid membrane HDA PubMed 
located_in cytosol HDA PubMed 
located_in intracellular anatomical structure TAS
Traceable Author Statement
more info
 PubMed 
part_of signal recognition particle, chloroplast targeting IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
chloroplast signal recognition particle component (CAO)
NP_566101.1
  • CHAOS (CAO); FUNCTIONS IN: chromatin binding; INVOLVED IN: response to high light intensity, protein import into chloroplast thylakoid membrane; LOCATED IN: chloroplast thylakoid membrane, chloroplast, signal recognition particle, chloroplast targeting, chloroplast envelope; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ankyrin repeat-containing domain (InterPro:IPR020683), Chromo domain-like (InterPro:IPR016197), Chromo domain (InterPro:IPR000953), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: ankyrin repeat-containing protein 2 (TAIR:AT4G35450.5); Has 25373 Blast hits to 13489 proteins in 848 species: Archae - 87; Bacteria - 2575; Metazoa - 11620; Fungi - 2006; Plants - 1049; Viruses - 137; Other Eukaryotes - 7899 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003071.7 Reference assembly

    Range
    19472573..19474387
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_130313.3 → NP_566101.1  chloroplast signal recognition particle component (CAO) [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_566101.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    O22265, Q93V50, Q940I7, Q9SAU3
    UniProtKB/TrEMBL
    A0A5S9X8K6
    Conserved Domains (5) summary
    smart00298
    Location:85 → 133
    CHROMO; Chromatin organization modifier domain
    cd00204
    Location:126 → 236
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    pfam00385
    Location:321 → 368
    Chromo; Chromo (CHRromatin Organization MOdifier) domain
    pfam12796
    Location:135 → 224
    Ank_2; Ankyrin repeats (3 copies)
    sd00045
    Location:129 → 157
    ANK; ANK repeat [structural motif]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O22265.2

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