MPK12 mitogen-activated protein kinase 12 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: MPK12
Official Full Name: mitogen-activated protein kinase 12
Primary source: TAIR:AT2G46070
Locus tag: AT2G46070
See related: Araport:AT2G46070
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATMPK12; MAPK12; mitogen-activated protein kinase 12; T3F17.28
Summary: Encodes a MAP kinase protein. MPK12 interacts with the IBR5 protein phosphatase in vitro and in vivo, and it can be dephosphorylated and inactivated by IBR5. MPK12 appears to be a negative regulator of auxin signlaing. MPK12 RNAi lines are hypersensitive to auxin in root elongation and transcriptional response assays, but they appear to have normal sensitivity to ABA. MPK12 is a nuclear protein and its kinase activity is increased following auxin treatment. MPK12 transcripts are widely expressed in seedlings, but MPK12 expression is stronger in guard cells than in other cell types in mature plants.
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Genomic context

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See MPK12 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 6

Sequence:: Chromosome: 2; NC_003071.7 (18945937..18948067, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

key component mediating CO2 -induced stomatal movements
Title: Mitogen-activated protein kinases MPK4 and MPK12 are key components mediating CO(2) -induced stomatal movements.
Study propose a model for sequential roles of MPK12, HT1, and GHR1 in the ABA-independent regulation of SLAC1 during CO2-induced stomatal closure.
Title: A Dominant Mutation in the HT1 Kinase Uncovers Roles of MAP Kinases and GHR1 in CO2-Induced Stomatal Closure.
MPK9 and MPK12 are positive regulators of salicylic acid signaling in Arabidopsis guard cells.
Title: MPK9 and MPK12 function in SA-induced stomatal closure in Arabidopsis thaliana.
MPK9 and MPK12 are key regulators mediating both abscisic acid (ABA) and Methyl jasmonate (MeJA) signalling in guard cells.
Title: Two guard cell mitogen-activated protein kinases, MPK9 and MPK12, function in methyl jasmonate-induced stomatal closure in Arabidopsis thaliana.
MPK9 and MPK12 function redundantly downstream of extracellular reactive oxygen production and intracellular accumulation, cytosolic alkalisation and Ca(2+)cytosolic oscillation in yeast elcictor-induced stomatal closure
Title: Two guard cell-preferential MAPKs, MPK9 and MPK12, regulate YEL signalling in Arabidopsis guard cells.
MPK9 and MPK12 act downstream of ROS and cytosolic Ca2+ and upstream of anion channels in the guard cell abscisic acid signaling cascade.
Title: Two Arabidopsis guard cell-preferential MAPK genes, MPK9 and MPK12, function in biotic stress response.
MAP kinases MPK9 and MPK12 are preferentially expressed in guard cells and positively regulate ROS-mediated ABA signaling.
Title: MAP kinases MPK9 and MPK12 are preferentially expressed in guard cells and positively regulate ROS-mediated ABA signaling.
MPK12 is both a physiological substrate of IBR5 and a novel negative regulator of auxin signaling.
Title: Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001202826.1

Supported by ESTs:

NM_130170.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables MAP kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to carbon dioxide	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular signal transduction	IC Inferred by Curator more info	PubMed
involved_in protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of stomatal closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of stomatal movement	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to auxin	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to indolebutyric acid	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IC Inferred by Curator more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: mitogen-activated protein kinase 12

NP_001189755.1

mitogen-activated protein kinase 12 (MPK12); CONTAINS InterPro DOMAIN/s: JNK MAP kinase (InterPro:IPR008351), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), Tyrosine-protein kinase, active site (InterPro:IPR008266), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635); BEST Arabidopsis thaliana protein match is: MAP kinase 4 (TAIR:AT4G01370.1).

NP_182131.2

mitogen-activated protein kinase 12 (MPK12); CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), JNK MAP kinase (InterPro:IPR008351), Serine/threonine-protein kinase domain (InterPro:IPR002290), Tyrosine-protein kinase, active site (InterPro:IPR008266), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009); BEST Arabidopsis thaliana protein match is: MAP kinase 4 (TAIR:AT4G01370.1); Has 125734 Blast hits to 124415 proteins in 4732 species: Archae - 106; Bacteria - 13752; Metazoa - 47505; Fungi - 12616; Plants - 30542; Viruses - 578; Other Eukaryotes - 20635 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

18945937..18948067 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001337177.1 → NP_001325006.1 mitogen-activated protein kinase 12 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178VX75, A0A1P8B1V3, A0A384LC88, A0A7G2EEE6

Conserved Domains (1) summary

cd07858
Location:68 → 404

STKc_TEY_MAPK; Catalytic domain of the Serine/Threonine Kinases, Plant TEY Mitogen-Activated Protein Kinases
NM_130170.4 → NP_182131.2 mitogen-activated protein kinase 12 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_182131.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O82361, Q8GYQ5

UniProtKB/TrEMBL

A0A7G2EEE6

Conserved Domains (1) summary

cd07858
Location:34 → 370

STKc_TEY_MAPK; Catalytic domain of the Serine/Threonine Kinases, Plant TEY Mitogen-Activated Protein Kinases
NM_001202826.1 → NP_001189755.1 mitogen-activated protein kinase 12 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001189755.1

Status: REVIEWED

UniProtKB/TrEMBL

F4IH84

Conserved Domains (2) summary

smart00220
Location:44 → 317

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl21453
Location:34 → 317

PKc_like; Protein Kinases, catalytic domain